Quantitating SARS-CoV-2 neutralizing antibodies from human dried blood spots DOI Creative Commons

Katherine Berman,

Greta Van Slyke,

Hayley Novak

et al.

Microbiology Spectrum, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 29, 2024

ABSTRACT In the earliest days of COVID-19 pandemic, collection dried blood spots (DBS) enabled public health laboratories to undertake population-scale seroprevalence studies used estimate rates SARS-CoV-2 exposure. With seropositivity levels now estimated exceed 94% in United States, attention has turned using DBS assess neutralizing antibodies within cohorts interest. this goal mind, we generated contrived (cDBS) and whole blood-derived from convalescent vaccinated individuals subjected eluates a battery assays, including multiplexed microsphere immunoassay (MIA), receptor binding domain (RBD)-human ACE2 inhibition assay (iACE2), cell-based pseudovirus neutralization assay, real-time PCR-based surrogate (NAB-Sure). The results were benchmarked against paired serum samples tested clinically validated plaque reduction titer (PRNT) assay. an 8-plex MIA NAB-Sure assays demonstrated highly significant correlations with PRNT values when evaluated panel 86 serum–DBS samples. Both are adaptable automated liquid handlers for high-throughput capacity. While limited ancestral WA1, study nonetheless represents important proof concept demonstrating potential utility as biospecimen type use assessing immunity at community population levels. IMPORTANCE variants concern continue circulate globally remain serious threat large segments population. From standpoint, identifying vulnerable communities based on immune status is critical terms vaccine booster recommendations. report, investigated which antibody titers. Using DBS, demonstrate that readily measurable correlate Moreover, several methods platforms.

Language: Английский

Inter-laboratory harmonization of microsphere immunoassays for SARS-CoV-2 antibody detection in contrived dried blood spots and oral fluids DOI Creative Commons
Kate L. DeRosa, Nora Pisanic, Kate Kruczynski

et al.

Microbiology Spectrum, Journal Year: 2025, Volume and Issue: unknown

Published: March 25, 2025

ABSTRACT Dried blood spots (DBS) and oral fluids (OF) are easily attainable biospecimen types that enabled population-scale antibody monitoring for SARS-CoV-2 exposure vaccination. However, the degree to which two different can be used interchangeably remains unclear. To address this question, we generated contrived DBS (cDBS) OF (cOF) from serum panels of SARS-CoV-2-infected, vaccinated, uninfected individuals. The samples were evaluated using multiplexed microsphere immunoassays (MIAs) at institutions. Intra-laboratory tests revealed near-perfect agreement between cDBS cOF N S antigens, as evidenced by κ = 0.97–1 98%–100% agreement. Interlaboratory comparisons equally robust both (κ 0.94–0.96; 97.5%–98 % agreement) 0.98–1.0; 99.0%–100%). Furthermore, assays transferred labs, including methods reagents, a subset ( n 52) tested. Qualitative concordance remained high 0.94–1.0; 97.5%–100% agreement), confirming integrity is retained upon transfer. In summary, our results provide evidence across laboratories institutions qualitative assessment determinations. IMPORTANCE establishment common research clinical laboratory methodologies levels in paramount importance estimating immunity COVID-19 infections communities populations. report, multiplex immunoassay protocols enable readily types, namely, dried (OF), employed titers virus-neutralizing potential.

Language: Английский

Citations

0
Neonatal Fc Receptor Participates in Endocytosis of Fc Fusion Protein In Vivo and In Vitro DOI Creative Commons
Yaping Zhou, Yanfang Wang, Hongmei Zhao

et al.

Veterinary Immunology and Immunopathology, Journal Year: 2025, Volume and Issue: unknown, P. 110930 - 110930

Published: April 1, 2025

Language: Английский

Citations

0
Factors modulating maternofetal transfer of IgG antibodies following SARS-CoV-2 gestational infection DOI Creative Commons
Aline Almeida Bentes, Vívian Mara Gonçalves de Oliveira Azevedo, Stela Maris Aguiar Lemos

et al.

Revista do Instituto de Medicina Tropical de São Paulo, Journal Year: 2025, Volume and Issue: 67

Published: Jan. 1, 2025

ABSTRACT Early infant immunity to SARS-CoV-2 depends on maternofetal transfer of antibodies. We aimed analyze the factors modulating anti-SARS-CoV-2 IgG antibodies following gestational infection during pandemic in Brazil (April–August 2021). conducted a retrospective and prospective cohort study involving 509 mother-child dyads tested simultaneously for anti-nucleocapsid universal neonatal screening. There were 341 seronegative 168 seropositive ones. Seropositive neonates retested two three months later. examined association serological status concentrations with mRNA vaccination, timing maternal infection, conditions, gender. Gestational predicted seropositivity (OR=3.97; 95%CI=2.69–5.88). Maternal first, second, or third trimester was associated progressively greater (34.4%, 51.6%, 58.2%, respectively; p=0.03). Among neonates, concentration higher when mothers reported they had COVID-19 pregnancy (p=0.04) tended be lower girls (p=0.06). More than half remained later (54.1%), which both at birth (p<0.001). Higher persistence anti-N more newborns. This provides an additional understanding dynamics antibody transfer.

Language: Английский

Citations

0
Use of Self-Collected Dried Blood Spots and a Multiplex Microsphere Immunoassay to Measure IgG Antibody Response to COVID-19 Vaccines DOI Creative Commons
Katherine L. Nemeth,

Erica Yauney,

Jean M. Rock

et al.

Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(1)

Published: Jan. 9, 2023

Serosurveys can determine the extent and spread of a pathogen in populations. However, collection venous blood requires trained medical staff. Dried spots (DBS) are suitable alternative because they be self-collected stored/shipped at room temperature. As COVID-19 vaccine deployment began early 2021, we rapidly enrolled laboratory employees study to evaluate IgG antibody levels following vaccination. Participants received DBS kit, self-collection instructions, brief questionnaire. Three were collected by each 168 participants pre- and/or postvaccination tested with multiplex microsphere immunoassay (MIA) that separately measures antibodies SARS-CoV-2 spike-S1 nucleocapsid antigens. Most (99.6%, 507/509) for testing. prior infection (

Language: Английский

Citations

7
Mass Spectrometry of Transferrin and Apolipoprotein CIII from Dried Blood Spots for Congenital Disorders of Glycosylation DOI Creative Commons
Yoshinao Wada,

Machiko Kadoya,

Nobuhiko Okamoto

et al.

Mass Spectrometry, Journal Year: 2022, Volume and Issue: 11(1), P. A0113 - A0113

Published: Dec. 8, 2022

Dried blood spot (DBS) is the standard specimen for newborn screening of inborn errors metabolism (IEM) by tandem mass spectrometry. Availability DBS spectrometric analysis diagnostic marker proteins, transferrin (Tf) and apolipoprotein CIII (apoCIII), congenital disorders glycosylation (CDG) was examined. Recovery Tf from only slightly reduced compared with fresh serum. Although oxidation core polypeptides observed, glycans apoCIII were unaffected storage in ambient environment at least 1 month. The combination triple quadrupole spectrometer used IEM sufficient to characterize aberrant glycoprofiles CDG. or dried serum on filter paper can reduce cost sample transportation potentially promote

Language: Английский

Citations

6
Development of a Method for Detection of SARS-CoV-2 Nucleocapsid Antibodies on Dried Blood Spot by DELFIA Immunoassay DOI Creative Commons
Verena Damiani,

Erika Pizzinato,

Ilaria Cicalini

et al.

Diagnostics, Journal Year: 2023, Volume and Issue: 13(5), P. 897 - 897

Published: Feb. 27, 2023

Antibodies against the SARS-CoV-2 nucleocapsid protein are produced by immune system in response to infection, but most available vaccines developed fight pandemic spread target spike protein. The aim of this study was improve detection antibodies providing a simple and robust method applicable large population. For purpose, we DELFIA immunoassay on dried blood spots (DBSs) converting commercially IVD ELISA assay. A total forty-seven paired plasma were collected from vaccinated and/or previously SARS-CoV-2-infected subjects. DBS-DELFIA resulted wider dynamic range higher sensitivity for detecting nucleocapsid. Moreover, showed good intra-assay coefficient variability 14.6%. Finally, strong correlation found between detected immunoassays (r = 0.9). Therefore, association sampling with technology may provide an easier, minimally invasive, accurate measurement In conclusion, these results justify further research develop certified assay useful diagnostics as well serosurveillance studies.

Language: Английский

Citations

3
Seroprevalence of SARS-CoV-2 antibodies in Italy in newborn dried blood spots DOI Creative Commons
Luisa Galla, Chiara Cosma,

Michela Bertan

et al.

Clinical Chemistry and Laboratory Medicine (CCLM), Journal Year: 2022, Volume and Issue: 61(2), P. 311 - 315

Published: Oct. 25, 2022

Serosurveys can be used to monitor COVID-19 seroprevalence and conduct surveillance. Dried blood spot (DBS), increasingly as a valuable sample assay severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) antibodies (Ab), has several advantages, particularly in infants, due the limited amount of required its utility testing large number samples time-frame. We evaluated SARS-CoV-2 IgG Ab prevalence newborn DBS Trentino region Italy, during time period January 2020 - December 2021.Anti-SARS-CoV-2 levels were determined by means Anti-SARS-CoV-2 QuantiVac ELISA (Euroimmun, Lubeck, Germany).Analyses included 2,400 from newborns (54% M, 46% F), being collected 2-3 days after birth. The first that tested positive for anti-SARS-CoV-2 was found March and, up May 2020, only 4 results detected overall. Starting June positivity thresholds increased according epidemiological waves pandemic with robust increment winters 2021. percentage rose 0 6% 10-47%, 2021, respectively.This study demonstrates is suitable tool both purposes surveillance pandemic, pregnant women, saving waste sparing patients any discomfort.

Language: Английский

Citations

4
Quantitating SARS-CoV-2 Neutralizing Antibodies from Human Dried Blood Spots DOI Creative Commons

Katherine Berman,

Greta Van Slyke,

Hayley Novak

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 20, 2024

ABSTRACT Background In the earliest days of COVID-19 pandemic, collection dried blood spots (DBS) enabled public health laboratories to undertake population-scale seroprevalence studies estimate rates SARS-CoV-2 exposure. With seropositivity levels now estimated exceed 94% in United States, attention has turned using DBS assess functional (neutralizing) antibodies within cohorts interest. Methods Contrived eluates from convalescent, fully vaccinated and pre-COVID-19 serum samples were evaluated plaque reduction neutralization titer (PRNT) assays, a specific 8-plex microsphere immunoassay, cell-based pseudovirus assay, two different spike-ACE2 inhibition an in-house Luminex-based RBD-ACE2 assay commercial real-time PCR-based (NAB-Sure™). Results convalescent individuals compatible with but not assays or PRNT. However, insensitivity was overcome high antibody titers. Conclusion neutralizing titers can be derived confidence eluates, thereby opening door use these biospecimens for analysis vulnerable populations normally hard reach communities.

Language: Английский

Citations

0
Use of dried blood spots in the detection of coronavirus disease 2019 (COVID-19): A systematic review DOI Open Access

Jannie Nikolai M Alquero,

Patrizia Marie S Estanislao,

Svethlana Marie M Hermino

et al.

Indian Journal of Medical Microbiology, Journal Year: 2024, Volume and Issue: 51, P. 100700 - 100700

Published: Aug. 13, 2024

COVID-19 disease continues to be a global health concern. The current protocol for detecting SARS-CoV-2 requires healthcare professionals draw blood from patients. Recent studies showed that dried spot (DBS) is valuable sampling procedure can collect low volume without the need presence of medical practitioners. This study synthesized available literature on using DBS as collection tool diagnose disease.

Language: Английский

Citations

0
Inter-laboratory harmonization of microsphere immunoassays for SARS-CoV-2 antibody detection in dried blood spots and oral fluids DOI Creative Commons
Kate L. DeRosa, Nora Pisanic, Kate Kruczynski

et al.

Published: Oct. 22, 2024

ABSTRACT Dried blood spots (DBS) and oral fluids (OF) are easily attainable biospecimen types that have enabled population scale antibody monitoring for SARS-CoV-2 exposure vaccination. However, the degree to which two different can be used interchangeably remains unclear. To begin address this question, we generated contrived DBS (cDBS) OF (cOF) from serum panels infected, vaccinated, uninfected individuals. The samples were evaluated using multiplexed microsphere immunoassays (MIAs) at institutions. Intra-laboratory tests revealed near perfect agreement between cDBS cOF N S antigens, as evidenced by κ = 0.97-1 98%-100% agreement. Inter-laboratory comparisons equally robust both (κ 0.94-0.96; 97.5%-98 % agreement) 0.98 -1.0; 99.0%-100%). Furthermore, assays transferred labs, including methods reagents, a subset of (n 52) tested. Qualitative concordance remained high 0.94-1.0; 97.5%-100% agreement), confirming integrity is retained upon transfer. In summary, our results provide evidence across laboratories institutions qualitative assessment determinations.

Language: Английский

Citations

0