Metabolomic profiling of cannabis use and cannabis intoxication in humans

Neuropsychopharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: March 12, 2025

Abstract Acute intoxication from Δ9-tetrahydrocannabinol (THC, the primary active ingredient of cannabis) can lead to neurocognitive impairment and interference with day-to-day operations, such as driving. Present evaluations THC-induced in legal settings rely on biological drug tests that solely establish cannabis use, rather than impairment. The current study evaluated metabolome blood collected occasional chronic users ( N = 35) at baseline following treatments (300 μg/kg THC) placebo, aim identify unique metabolic alterations are associated acute use frequency. Blood samples were repeatedly during 70 min after treatment. Sustained attention performance ratings subjective high taken twice within 40 Metabolomic fingerprints distinctly different baseline, when both groups not intoxicated. A total 14 metabolites, mainly related endocannabinoid amino acid metabolism, identified distinguished yielded a discriminant analysis model an 80% classification rate (95% CI: 61–91%). Distinct metabolomic found for who, contrast users, showed attentional elevated intoxication. These included increments organic acids, β-hydroxybutyrate second messenger ceramides. demonstrates feasibility metabolomics approach changes specific state history use.

Language: Английский

Intoxication due to Δ9-tetrahydrocannabinol is characterized by disrupted prefrontal cortex activity

Neuropsychopharmacology, Journal Year: 2024, Volume and Issue: 49(9), P. 1481 - 1490

Published: May 7, 2024

Abstract Neural states of impairment from intoxicating substances, including cannabis, are poorly understood. Cannabinoid 1 receptors, the main target Δ9-tetrahydrocannabinol (THC), primary cannabinoid in densely localized within prefrontal cortex; therefore, brain regions key locations to examine changes that characterize acute intoxication. We conducted a double-blind, randomized, cross-over study adults, aged 18–55 years, who use cannabis regularly, determine effects intoxication on cortex resting-state measures, assessed with portable functional near-infrared spectroscopy. Participants received oral THC (10–80 mg, individually dosed overcome tolerance and achieve intoxication) identical placebo, randomized for order; 185 adults were 128 completed both days had usable data. was associated expected increases subjective ratings ( ES = 35.30, p < 0.001) heart rate 11.15, 0.001). decreased correlations anticorrelations static connectivity relative weakest among those reported greater severity (RSFC between medial PFC-ventromedial PFC DEQ scores, r 0.32, 0.001; RSFC bilateral mPFC –0.28, Relative increased variability (or reduced stability) dynamic at 0.001, consistent across range window sizes. Finally, using frequency power spectrum analyses, we observed widespread spectral 0.073–0.1 Hz 0.039. These neural features suggest disruptive influence dynamics may underlie cognitive impairing detectable imaging. This is registered Clinicaltrials.gov (NCT03655717).

Language: Английский

Road hazard: a systematic review of traffic injuries following recreational cannabis legalization

Canadian Journal of Emergency Medicine, Journal Year: 2024, Volume and Issue: 26(8), P. 554 - 563

Published: July 1, 2024

Language: Английский