Virology, Journal Year: 2023, Volume and Issue: 582, P. 114 - 127
Published: April 5, 2023
Language: Английский
Frontiers in Immunology, Journal Year: 2022, Volume and Issue: 13
Published: Sept. 13, 2022
ISG20 inhibits viruses such as SARS-CoV-2 invasion; however, details of its expression and regulation with viral susceptibility remain to be elucidated. The present study analyzed expression, isoform information, survival rate, methylation patterns, immune cell infiltration, COVID-19 outcomes in healthy cancerous individuals. Cordycepin (CD) N6, N6-dimethyladenosine (m62A) were used treat cancer cells for expression. We revealed that mRNA was primarily located the bone marrow lymphoid tissues. Interestingly, significantly increased 11 different types cancer, indicating patients may less vulnerable infection. Among them, higher associated a long OS CESC SKCM, suggesting good marker both prevention progress. promoter lower BLCA, READ, THCA tumor tissues than matched normal tissues, while BRCA, LUSC, KIRC, PAAD. Hypermethylation KIRC PAAD correlated ISG20, not underlie overexpression. Furthermore, infiltration levels, including lymphocytes, chemokine, receptors, immunoinhibitors, immunostimulators, MHC molecules pan-cancer. STAD exhibited highest degree mutations; median progression-free time months unaltered group 61.84, it 81.01 mutant group. Isoforms ISG20-001 ISG20-009 showed same RNase_T domain structure, demonstrating functional roles tumorigenesis invasion inhibition patients. Moreover, CD m62A increase various lines, implying antiviral/anti-SARS-CoV-2 therapeutic potential. Altogether, this highlighted value combating by targeting during pandemic, small extracted from traditional Chinese medicines, CD, have potential anti-SARS-CoV-2 anticancer agents promoting
Language: Английский
Citations
6
Microbiology Spectrum, Journal Year: 2022, Volume and Issue: 10(6)
Published: Nov. 29, 2022
Animal experiments on African swine fever virus (ASFV) are vital to the study of ASFV; however, ASFV can only infect pigs, and animal need be performed in biosafety level 3 (ABSL-3) laboratories, meaning that many small ABSL-3 laboratories unable carry out vivo experiments. Therefore, miniaturized experimental animals for infection urgently needed. Here, we successfully isolated genotype II SY-1 from wild boars evaluated ASFV-infected Bama minipigs a negative-pressure isolator laboratory. The pathological changes were consistent with characteristic lesions domestic pigs boars. All died 5 14 days postinfection (dpi) through intramuscular injection. Viral genomic DNA nasal, oral, rectal swab samples was first detectable at 2 4 dpi. common differentially expressed genes clustered immune-related, metabolic, inflammatory response pathways spleen inguinal lymph node comparing infected mock. In summary, these results demonstrated minipig an appropriate model accelerate research vaccines antiviral drugs uncover pathogenic mechanisms infection. IMPORTANCE rather than other animals. However, cannot kept long time due characteristics rapid growth large size, which hinder research, including vaccines, drugs, mechanisms. contrast, have unique advantages consisting low size. used evaluate laboratories. changes, viral shedding, gene regulation those ASFV. suitable
Language: Английский
Citations
6
Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)
Published: Dec. 13, 2023
Abstract Among the causative agents of neonatal diarrhoea in calves, two most prevalent are bovine coronavirus (BCoV) and intracellular parasite Cryptosporidium parvum . Although several studies indicate that co-infections associated with greater symptom severity, host–pathogen interplay remains unresolved. Here, our main objective was to investigate modulation transcriptome HCT-8 cells during single BCoV C. For this, were inoculated (1) alone, (2) alone , (3) simultaneously. After 24 72 h, harvested analyzed using high-throughput RNA sequencing. Following differential expression analysis, over 6000 differentially expressed genes (DEGs) identified virus-infected co-exposed at hpi, whereas only 52 DEGs found -infected same time point. Pathway (KEGG) gene ontology (GO) analysis showed mostly immune pathways (such as NF-κB, TNF-α or, IL-17), apoptosis regulation transcription, a more limited effect exerted by observed co-infection apparently dominated virus, 800 uniquely hpi. Our findings provide insights on possible biomarkers co-infection, which could be further explored vivo models.
Language: Английский
Citations
3
Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)
Published: Dec. 6, 2023
Natural products and herbal medicine have been widely used in drug discovery for treating infectious diseases. Recent outbreak of COVID-19 requires various therapeutic strategies. Here, we YSK-A, a mixture three components Boswellia serrata, Commiphora myrrha, propolis, to evaluate potential antiviral activity against SARS-CoV-2. We showed that YSK-A inhibited SARS-CoV-2 propagation with an IC50 values 12.5 µg/ml 15.42 Vero E6 Calu-3 cells, respectively. Using transcriptome analysis, further demonstrated modulated host gene expressions cells. Among these, selected 9 antiviral- or immune-related genes study. By siRNA-mediated knockdown experiment, verified MUC5AC, LIF, CEACAM1, GDF15 were involved YSK-A. Therefore, silencing these nullified YSK-A-mediated inhibition propagation. These data indicate displays anti-SARS-CoV-2 by targeting multiple genes. Although the exact mechanism each constituent has not yet, our immunomodulatory effect on thus it may represent novel natural product-derived agent COVID-19.
Language: Английский
Citations
2
Virology, Journal Year: 2023, Volume and Issue: 582, P. 114 - 127
Published: April 5, 2023
Language: Английский
Citations
1