Strengthening the bond with the scientific community: FEBS Open Bio in 2025
FEBS Open Bio,
Journal Year:
2025,
Volume and Issue:
15(1), P. 4 - 10
Published: Jan. 1, 2025
FEBS
Open
Bio
remains
dedicated
to
serving
the
scientific
community
by
ensuring
rapid
publication
of
rigorous
science
and
pioneering
initiatives
support
researchers.
In
this
editorial,
we
reflect
on
a
year
achievements,
look
forward
new
developments
planned
for
2025.
Language: Английский
Mutational analysis of the role of conserved histidine residues of the SDHC subunit of Complex II
Research Square (Research Square),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 16, 2025
Abstract
Background:
SDHC
protein
is
a
critical
component
of
the
succinate-ubiquinone
oxidoreductase
also
known
as
respiratory
chain
Complex
II.
Because
mutations
are
associated
with
several
human
diseases,
detailed
understanding
role
in
II
and
overall
cellular
physiology
important.
In
this
study,
we
have
tested
functional
complementation
SDHC-null
cells
different
variants
proteins
fused
GFP
at
C-terminus.
Specifically,
determined
four
conserved
histidines
(H55,
H71,
H127,
H134)
on
function
mitochondrial
morphology.
Methods:
The
SDHC-deficient
Chinese
hamster
cell
line
CCL16-B9
was
transfected
plasmid
constructs
expressing
(SDHC,
SDHC∆,
SDHC-H55N,
SDHC-H71N,
SDHC-H127N,
SDHC-H134N)
either
or
HA-GFP
Restoration
by
galactose
selection,
biochemical
assays,
Western
blotting,
assessment
morphology
fluorescence
microscopy.
Results:
key
findings
study
are:
(1)
C-terminus
does
not
interfere
import,
orientation,
function.
(2)
Wild
type
mutant
SDHCs
can
deliver
into
IMS.
(3)
third
transmembrane
domain
essential
for
(4)
While
H55,
H71are
non-essential,
H127
H134
H127N
H134N
impair
alter
most
detrimental
effects
due
to
mutation.
(5)
serve
carrier
delivering
interest
Conclusions:
This
advances
our
subunit
II,
morphology,
physiology.
Language: Английский
Understanding mitochondrial protein import: a revised model of the presequence translocase
Trends in Biochemical Sciences,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 1, 2025
Mitochondrial
function
relies
on
the
precise
targeting
and
import
of
cytosolic
proteins
into
mitochondrial
subcompartments.
Most
matrix-targeted
follow
presequence
pathway,
which
directs
precursor
across
outer
membrane
(OMM)
via
Translocase
Outer
Membrane
(TOM)
complex
matrix
or
inner
(IMM)
Inner
23
(TIM23)
complex.
While
classical
biochemical
studies
provided
detailed
mechanistic
insights
composition
mechanism
TIM23
complex,
recent
cryogenic-electron
microscopy
(cryo-EM)
data
challenge
these
established
models
propose
a
revised
model
translocation
in
TIM17
subunit
acts
as
'slide'
for
proteins,
with
Tim23
acting
structural
element.
In
this
review,
we
summarize
existing
models,
highlighting
questions
needed
to
reconcile
perspectives,
enhance
our
understanding
function.
Language: Английский
Mitochondria: the beating heart of the eukaryotic cell
FEBS Open Bio,
Journal Year:
2024,
Volume and Issue:
14(10), P. 1588 - 1590
Published: Oct. 1, 2024
Mitochondria
are
essential
organelles
of
eukaryotic
cells.
They
consist
hundreds
proteins,
which
synthesized
in
the
cytosol
and
imported
into
mitochondria
via
different
targeting
routes.
In
addition,
a
small
number
proteins
encoded
by
organellar
genome
mitochondrial
ribosomes.
this
‘In
Limelight’
special
issue
FEBS
Open
Bio
,
five
review
articles
describe
these
biogenesis
routes
provide
comprehensive
overview
structures
mechanisms
transported
to
their
respective
location
within
organelle.
These
reviews,
written
leading
experts,
general
overview,
but
also
highlight
current
developments
field
biogenesis.
Language: Английский