Comparison of Conjugates Obtained Using DMSO and DMF as Solvents in the Production of Polyclonal Antibodies and ELISA Development: A Case Study on Bisphenol A DOI Creative Commons
Anna N. Berlina, Nadezhda S. Komova, Kseniya V. Serebrennikova

et al.

Antibodies, Journal Year: 2024, Volume and Issue: 13(4), P. 89 - 89

Published: Oct. 29, 2024

When developing immunochemical test systems, it is necessary to obtain specific antibodies. Their quality depends, among other things, on the immunogen used. preparing hapten–protein conjugates antibodies for low-molecular-weight compounds, key factors are structure of hapten itself, presence a spacer, size carrier protein and degree its modification by molecules. This work shows that one additional factor—the conditions obtaining conjugate—is overlooked. In this work, we have synthesized bisphenol A derivative 4,4-bis(hydroxyphenyl)valeric acid (BVA), soybean trypsin inhibitor (STI), bovine serum albumin (BSA) in reaction media combining water with two organic solvents: dimethylformamide (DMF) or dimethyl sulfoxide (DMSO). Namely, BSADMF–BVA, STIDMF–BVA, BSADMSO–BVA STIDMSO–BVA were obtained. Rabbit polyclonal against BSADMF–BVA conjugate demonstrated basically different interactions developed ELISA systems using either STIDMF–BVA conjugates. The use absence competition combination antisera obtained from an ELISA. competitive interaction was observed only conjugate. Under selected conditions, detection limit 8.3 ng/mL, working range determined concentrations 18.5–290.3 ng/mL. data demonstrate possibility achieving sensitive immunoassays simply varying conjugation, which could provide tool development compounds.

Language: Английский

Comparison of Conjugates Obtained Using DMSO and DMF as Solvents in the Production of Polyclonal Antibodies and ELISA Development: A Case Study on Bisphenol A DOI Creative Commons
Anna N. Berlina, Nadezhda S. Komova, Kseniya V. Serebrennikova

et al.

Antibodies, Journal Year: 2024, Volume and Issue: 13(4), P. 89 - 89

Published: Oct. 29, 2024

When developing immunochemical test systems, it is necessary to obtain specific antibodies. Their quality depends, among other things, on the immunogen used. preparing hapten–protein conjugates antibodies for low-molecular-weight compounds, key factors are structure of hapten itself, presence a spacer, size carrier protein and degree its modification by molecules. This work shows that one additional factor—the conditions obtaining conjugate—is overlooked. In this work, we have synthesized bisphenol A derivative 4,4-bis(hydroxyphenyl)valeric acid (BVA), soybean trypsin inhibitor (STI), bovine serum albumin (BSA) in reaction media combining water with two organic solvents: dimethylformamide (DMF) or dimethyl sulfoxide (DMSO). Namely, BSADMF–BVA, STIDMF–BVA, BSADMSO–BVA STIDMSO–BVA were obtained. Rabbit polyclonal against BSADMF–BVA conjugate demonstrated basically different interactions developed ELISA systems using either STIDMF–BVA conjugates. The use absence competition combination antisera obtained from an ELISA. competitive interaction was observed only conjugate. Under selected conditions, detection limit 8.3 ng/mL, working range determined concentrations 18.5–290.3 ng/mL. data demonstrate possibility achieving sensitive immunoassays simply varying conjugation, which could provide tool development compounds.

Language: Английский

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