Selective Electrochemical Biosensors for Tyrosinase and Tyramine via Enzyme-Mediated Oxidation Reaction DOI
Islam M. Mostafa, Abubakar Abdussalam, Hao Jiang

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 6, 2025

Tyrosinase, a copper-containing oxidase, holds promise as critical biomarker for diverse skin-related infections and vitiligo melanoma tumors. Beyond its role in biological pigmentation, tyrosinase is also involved the oxidation of biogenic amines such tyramine. Tyramine substrate serves precursor synthesis various neurotransmitters dopamine norepinephrine contributes to significant neurological processes. It well-stated that could oxidize tyramine presence oxygen give product. The by produce has been widely applied colorimetric fluorimetric assays tyrosinase. Herein, we optimized utilized this reaction effective electrochemical detection without any electrode modification first time. We achieved selective via situ, generated from enzymatic conversion Tyrosinase were detected using cyclic voltammetry (CV) square wave (SWV). Both biosensors demonstrated excellent analytical performance. CV offered linear ranges 0.7-10 μg/mL 5.0-100 μM tyramine, respectively, while SWV exhibited notably low signal-to-noise ratio, resulting enhanced sensitivity (0.3-10 LOD 0.29 μg/mL) (1.0-100 0.69 μM). simple convenient successfully human serum samples yogurt urine are very promising real samples.

Language: Английский

Selective Electrochemical Biosensors for Tyrosinase and Tyramine via Enzyme-Mediated Oxidation Reaction DOI
Islam M. Mostafa, Abubakar Abdussalam, Hao Jiang

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 6, 2025

Tyrosinase, a copper-containing oxidase, holds promise as critical biomarker for diverse skin-related infections and vitiligo melanoma tumors. Beyond its role in biological pigmentation, tyrosinase is also involved the oxidation of biogenic amines such tyramine. Tyramine substrate serves precursor synthesis various neurotransmitters dopamine norepinephrine contributes to significant neurological processes. It well-stated that could oxidize tyramine presence oxygen give product. The by produce has been widely applied colorimetric fluorimetric assays tyrosinase. Herein, we optimized utilized this reaction effective electrochemical detection without any electrode modification first time. We achieved selective via situ, generated from enzymatic conversion Tyrosinase were detected using cyclic voltammetry (CV) square wave (SWV). Both biosensors demonstrated excellent analytical performance. CV offered linear ranges 0.7-10 μg/mL 5.0-100 μM tyramine, respectively, while SWV exhibited notably low signal-to-noise ratio, resulting enhanced sensitivity (0.3-10 LOD 0.29 μg/mL) (1.0-100 0.69 μM). simple convenient successfully human serum samples yogurt urine are very promising real samples.

Language: Английский

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