Translational Research in Anatomy,
Journal Year:
2022,
Volume and Issue:
26, P. 100169 - 100169
Published: Jan. 30, 2022
For
translational
research
on
lung
diseases
it
is
desirable
to
have
human
organoids
which
resemble
features
of
tissue.
Adult
stem
cells
obtained
from
bronchial
epithelium
can
be
differentiated
little
tissue
follicles,
so-called
bronchospheres.
Aim
the
study
was
characterize
structural
details
bronchospheres
treated
with
control
media,
lipopolysaccharide,
and
flagellin
during
differentiation
phase.
Human
epithelial
were
prepared
surgical
specimens
in
Matrigel
Histology
transmission
electron
microscopy
revealed
cellular
structure
Follicles
a
diameter
about
120
µm
confined
by
bilayered
surface
towards
inner
lumen
follicle
junctional
complexes.
Microvilli,
kinocilia
as
well
secretory
found.
Treatment
bacterial
resulted
formation
granules
inhibited
occurrence
kinocilia.
Despite
clear
limitations
such
lacking
smooth
muscles
columnar
are
an
interesting
tool
for
research.
They
useful
studies
addressing
developmental
processes,
infections,
drug
testing
toxicology.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: April 3, 2023
Abstract
Human
lung
organoids
(HLOs)
are
increasingly
used
to
model
development
and
infectious
diseases,
however
their
ability
recapitulate
functional
pulmonary
tissue
response
nanomaterial
(NM)
exposures
has
yet
be
demonstrated.
Here,
we
established
a
organoid
exposure
that
utilises
microinjection
present
NMs
into
the
lumen
of
organoids.
Our
assures
efficient,
reproducible
controllable
apical
epithelium,
emulating
real-life
human
scenario.
By
comparing
impact
two
well
studied
carbon-based
NMs,
graphene
oxide
sheets
(GO)
multi-walled
carbon
nanotubes
(MWCNT),
validated
as
tools
for
predicting
NM-driven
responses.
In
agreement
with
in
vivo
data,
demonstrate
MWCNT,
but
not
GO,
elicit
adverse
effects
on
organoids,
leading
pro-fibrotic
phenotype.
findings
reveal
capacity
suitability
HLOs
hazard
assessment
aligned
much
sought-out
3Rs
(animal
research
replacement,
reduction,
refinement)
framework.
In vitro models,
Journal Year:
2023,
Volume and Issue:
2(6), P. 249 - 262
Published: June 29, 2023
Human
induced
pluripotent
stem
cell
(hiPSC)-derived
lung
types
such
as
alveolar
epithelial
cells
are
promising
for
toxicological
and
pharmaceutical
in
vitro
screenings.
Reproducible
differentiation
processes
highly
demanded,
but
protocols
which
suitable
the
high-throughput
generation
of
from
hiPSCs
lacking.
In
this
study,
a
new
approach
hiPSC-differentiation
epithelial-like
type
2
under
dynamic
3D-conditions
suspension
bioreactor
is
presented.
Gene
protein
expression
analyses
key
markers
during
embryonal
development
have
been
performed
comparison
to
differentiated
static
2D-conditions
evaluate
efficacy
bioreactor-based
approach.
Finally,
resulting
were
infected
by
SARS-CoV-2
pseudotypes
demonstrate
their
functionality
suitability
e.g.
COVID-19
drug
development.
The
differentiate
spheroids,
express
relevant
each
developmental
stage
on
gene
level.
3D
method
able
significantly
increase
some
conventional
2D
differentiation.
3D-differentiated
functional
receptors
can
display
viral
infection.
presented
3D-differentiation
promising,
generate
source
models.
online
version
contains
supplementary
material
available
at
10.1007/s44164-023-00052-1.
Biomedicines,
Journal Year:
2022,
Volume and Issue:
10(11), P. 2764 - 2764
Published: Oct. 31, 2022
The
progress
of
medical
technology
and
scientific
advances
in
the
field
anticancer
treatment
have
increased
survival
probabilities
duration
life
patients.
However,
cancer-therapy-induced
cardiac
dysfunction
remains
a
clinically
salient
problem.
Effective
therapies
may
eventually
induce
cardiomyopathy.
To
date,
several
studies
focused
on
mechanisms
underlying
cancer-treatment-related
cardiotoxicity.
Cardiomyocyte
cell
lines
with
no
contractile
physiological
characteristics
cannot
adequately
model
"true"
human
cardiomyocytes.
applying
cardiomyocytes
for
research
is
fraught
many
obstacles
(e.g.,
invasiveness
procedure),
there
proliferative
limitation
rodent
primary
cultures.
Human-induced
pluripotent
stem-cell-differentiated
(hiPSC-CMs),
which
can
be
produced
efficiently,
are
viable
candidates
mimicking
vitro.
We
successfully
performed
differentiation
iPSCs
to
obtain
hiPSC-CMs.
These
hiPSC-CMs
used
investigate
pathophysiological
basis
molecular
mechanism
cardiotoxicity
develop
novel
strategies
prevent
rescue
such
propose
that
as
an
vitro
drug
screening
platform
study
targeted
cancer-therapy-related
Translational Research in Anatomy,
Journal Year:
2022,
Volume and Issue:
26, P. 100169 - 100169
Published: Jan. 30, 2022
For
translational
research
on
lung
diseases
it
is
desirable
to
have
human
organoids
which
resemble
features
of
tissue.
Adult
stem
cells
obtained
from
bronchial
epithelium
can
be
differentiated
little
tissue
follicles,
so-called
bronchospheres.
Aim
the
study
was
characterize
structural
details
bronchospheres
treated
with
control
media,
lipopolysaccharide,
and
flagellin
during
differentiation
phase.
Human
epithelial
were
prepared
surgical
specimens
in
Matrigel
Histology
transmission
electron
microscopy
revealed
cellular
structure
Follicles
a
diameter
about
120
µm
confined
by
bilayered
surface
towards
inner
lumen
follicle
junctional
complexes.
Microvilli,
kinocilia
as
well
secretory
found.
Treatment
bacterial
resulted
formation
granules
inhibited
occurrence
kinocilia.
Despite
clear
limitations
such
lacking
smooth
muscles
columnar
are
an
interesting
tool
for
research.
They
useful
studies
addressing
developmental
processes,
infections,
drug
testing
toxicology.
