Preparation of Carbon Dots with Peroxidase-like Activity and Their Application in Staphylococcus aureus Detection and Antimicrobial Susceptibility Test

Langmuir, Journal Year: 2025, Volume and Issue: unknown

Published: March 3, 2025

Carbon dot (CD) nanozymes with excellent biocompatibility, optical properties, and catalytic activity show great promise for microbial detection drug sensitivity testing. This study reports the synthesis of metal-doped green-emitting CDs good peroxidase-like activity, which were synthesized via a one-step hydrothermal route using thiourea, N-[3-(trimethoxysilyl)propyl]ethylenediamine, catechol as starting materials FeCl3 doping agent. In presence H2O2, catalyze oxidation 3,3',5,5'-tetramethylbenzidine (TMB), producing blue product; however, in bacteria bacterial catalase enzyme decomposes H2O2 inhibits CDs, preventing color change. The neutralizes prevents from color-changing reaction TMB. Based on CDs-TMB-H2O2 cascade system bioenzymes nanozymes, we developed rapid, sensitive, direct colorimetric method Staphylococcus aureus (S. aureus) limit 2 × 103 CFU/mL linear range 103-2 106 CFU/mL. visual was successfully applied to S. food samples. Antibiotics have different effects proliferation sensitive resistant strains, leading levels hydrolysis solution resulting varying intensities color; therefore, simple antibiotic susceptibility test. applications CD provide powerful tool detecting pathogenic food, clinical, environmental samples infections.

Language: Английский

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A ‘Two Birds with one stone’ strategy via α-Fe2O3 confined-domain growth and defect engineering for enhanced amphiphilic pollutant removal: mechanistic insights

Separation and Purification Technology, Journal Year: 2025, Volume and Issue: unknown, P. 132989 - 132989

Published: April 1, 2025

Language: Английский

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Construction of Lignin‐Assisted Nitrogen Defective Engineering Graphitic Carbon Nitride for HMF Selective Conversion to Maleic Acid Through Photo‐Thermal Processes

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 2, 2025

Abstract Design engineering for the preparation of green and inexpensive catalysts assisted by lignocellulosic biomass or biomass‐based derivatives has garnered significant attention. Herein, alkali lignin (AL) is utilized as a carbon precursor to prepare porous coral‐like g‐C 3 N 4 (x% PLCN) with nitrogen defects biochar persistent free radicals (biochar‐PFRs). This catalyst applied photo‐thermal catalysis HMF produce maleic acid (MA). The optimal yield MA (50.43%) achieved using 1.5% PLCN in GVL/FAc (1:1, V/V) solution at 90 °C h under visible light irradiation. Results show plays key role forming components introducing into catalysts, which significantly boosts H 2 O generation (156.68 µmol L −1 ). Under photocatalytic conditions, biochar‐PFRs induce ·OH through metal‐free Fenton‐like reaction provide active sites recombination ·O − form 1 . presence greatly enhances selective MA. Additionally, increased Lewis acidity x% activates C─C bond thermal catalytic further facilitating conversion. work presents new approach material refinement thermal‐photo catalysis.

Language: Английский

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Unveiling the reaction mechanism of photocatalytic H2 evolution coupled with selective bio-mass monosaccharide upgrading over single palladium atom-engineered carbon-rich graphitic carbon nitride

Journal of Colloid and Interface Science, Journal Year: 2025, Volume and Issue: 686, P. 1055 - 1073

Published: Feb. 6, 2025

Language: Английский

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Enhanced Nitrogen Vacancy Density Induced by Sulfur Doping on Carbon Nitride Facilitates Selective Lactic Acid Production

Published: Jan. 1, 2025

Language: Английский

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