Functionalization of Polyethylene Terephthalate (PETE) Membranes for the Enhancement of Cellular Adhesion in Organ-on-a-Chip Devices

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 7, 2025

Experimental reproducibility in organ-on-chip (OOC) devices is a challenging issue, mainly caused by cell adhesion problems, as OOC are made of bioinert materials not suitable for natural cellularization their surfaces. To improve adhesion, several surface functionalization techniques have been proposed, among which the simple use an intermediate layer adsorbed proteins has become preferred one users. This way, cells receptors to adhere proteins, turn attached surface. However, protein adsorption based on weak electrostatic bonding between coating and substrate, this method produces suboptimal results: bonds break, detach, leading poor, heterogeneous cellularization. solve problem, we present polyethylene terephthalate (PETE) membranes, commonly used multilayer support cellular layers. protocol involves hydrolyzation membrane, followed (3-dimethylaminopropyl) carbodiimide (EDC) N-hydroxysuccinimide (NHS) activation, resulting covalent membrane much stronger than provided adsorption. As evaluation, first measured effect morphological mechanical integrity membranes. Next, confirmed efficiency using ζ potential tension functionalized membranes coated with collagen type I, polylysine, gelatin, albumin, fetal bovine serum (FBS), Matrigel. Finally, showed that our significantly improves attachment epithelial (A549) endothelial (EA.hy926) lines under static conditions, especially collagen-coated were further tested dynamic showing statistically significant improvement compared uncoated or collagen-adsorbed only

Language: Английский

Engineering advanced in vitro models of endothelial dysfunction

Trends in biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Interfacial Engineering for Biomolecule Immobilisation in Microfluidic Devices

Biomaterials, Journal Year: 2024, Volume and Issue: 316, P. 123014 - 123014

Published: Dec. 15, 2024

Language: Английский