Biomaterials meet organ-on-chips – a perspective on tumor modeling DOI
Cátia F. Monteiro, Inês A. Deus, Catarina A. Custódio

et al.

International Materials Reviews, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 10, 2024

Microfluidic organ-on-chip systems are promising platforms for the development of biomimetic models that aim to reconstruct 3D architecture and intrinsic functionality native tissues. An in-depth comprehension pivotal role extracellular matrix in intricate cellular responses has paved way emergence biologically-relevant instructive biomaterials can capture essence cell's microenvironment. The notable evolution realm toward more realistic vitro tissue capable recreating synergistic cell-extracellular interplay is covered. overview most recent advances integrating materials into provided, including exploitation bulk hydrogels as soft material devices fulfill requirements direct cell-matrix interaction. successful application this cutting-edge technology on tumor modeling then discussed, highlighting great contribution perfusable microvessels elucidate mechanistic events metastatic cascade. This convergence science with organ-on-a-chip envisioned foster understanding behavior, shedding light dynamism interactions.

Language: Английский

Temperature-responsive two-dimensional polydopamine hydrogel: Preparation, mechanisms, and applications in cancer treatment DOI

Tiantian Gong,

Jiahui Jiang, Cheng Chen

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 282, P. 136891 - 136891

Published: Oct. 28, 2024

Language: Английский

Citations

4
Novel hydrogel-based cancer-on-a-chip models for growth of 3D multi-cellular structures and investigation of early angiogenesis in pancreatic ductal adenocarcinoma DOI Creative Commons

Karinna Georgiana Pele,

Alejandro Calderón-Villalba, H. Amaveda

et al.

Colloids and Surfaces B Biointerfaces, Journal Year: 2025, Volume and Issue: 253, P. 114736 - 114736

Published: May 1, 2025

Cancer-on-a-chip models have enormous potential for the study of tumour development events. Here, we investigated hydrogels egg white (EW) and gelatin growth 3D multi-cellular structures investigation early angiogenesis inside microfluidic devices. We focused on pancreatic ductal adenocarcinoma (PDAC), a devastating gastrointestinal malignancy. EW/gelatin were stiffer showed porous globular compared to fibrous network collagen I molecules. PANC-1 cells preferentially formed significantly larger spheroids in than hydrogels, whilst cell aggregates shape grape-like clusters more abundant EW/gelatin. Cells active unions, secreted matrix, unions with surrounding hydrogel. Early stages PDAC recreated by co-culture two different microenvironments, one another fibroblasts, investigating secretion soluble angiogenic factors, which depended role each factor tumorigenic processes. Overall, cancer proliferation establishment vasculature favoured. This demonstrates importance microenvironment behaviour as well complex interplay between present establish tumoural vasculature.

Language: Английский

Citations

0
Human Platelet Lysate-Derived Nanofibrils as Building Blocks to Produce Free-Standing Membranes for Cell Self-Aggregation DOI Creative Commons
Cátia F. Monteiro, Maria C. Gomes, Pankaj Bharmoria

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(24), P. 15815 - 15830

Published: June 4, 2024

Amyloid-like fibrils are garnering keen interest in biotechnology as supramolecular nanofunctional units to be used biomimetic platforms control cell behavior. Recent insights into fibril functionality have highlighted their importance tissue structure, mechanical properties, and improved adhesion, emphasizing the need for scalable high-kinetics synthesis. In this study, we present instantaneous bulk formation of amyloid-like nanofibrils from human platelet lysate (PL) using ionic liquid cholinium tosylate a fibrillating agent. The instant fibrillation PL proteins upon protein–ionic interactions was confirmed protein conformational transition toward cross-β-sheet-rich structures. These were utilized building blocks thin flexible free-standing membranes via solvent casting support self-aggregation. PL-derived reveal nanotopographically rough surface high stability over 14 days under culture conditions. mesenchymal stem cells or tumor on top membrane demonstrated that able adhere self-organize three-dimensional (3D) spheroid-like microtissue while tightly folding membrane. Results suggest nanofibril incorporation aggregates can improve viability metabolic activity, recreating native tissues' organization. Altogether, these suitable bioactive generate 3D cell-guided microtissues, which explored bottom-up strategies faithfully emulate tissues fully microenvironment.

Language: Английский

Citations

2
Modeling 3D Tumor Invasiveness to Modulate Macrophage Phenotype in a Human‐Based Hydrogel Platform DOI
Cátia F. Monteiro, Catarina R. Almeida, Catarina A. Custódio

et al.

Macromolecular Bioscience, Journal Year: 2024, Volume and Issue: 24(11)

Published: June 28, 2024

Abstract The immune system is a pivotal player in determining tumor fate, contributing to the immunosuppressive microenvironment that supports progression. Considering emergence of biomaterials as promising platforms mimic microenvironment, human platelet lysate (PLMA)‐based hydrogel beads are proposed 3D recapitulate milieu and recreate synergistic tumor‐macrophage communication. Having characterized biomaterial‐mediated pro‐regenerative macrophage phenotype, an osteosarcoma spheroid encapsulated into PLMA bead explored study immunomodulation through paracrine signaling. culture PLMA‐Tumor on top 2D monolayer macrophages reveals cells triggered morphologic metabolic adaptations macrophages. cytokine profile, coupled with upregulation gene protein anti‐inflammatory biomarkers clearly indicates polarization toward M2‐like phenotype. Moreover, increased expression chemokines identified pro‐tumoral environmental regulators suggest tumor‐associated exclusively stimulated by cells. This also found enhance invasiveness ability proliferation. Besides providing robust vitro immunomodulatory model faithfully recreates interplay, this human‐based platform has potential provide fundamental insights signaling predict immune‐targeted response.

Language: Английский

Citations

1
Biomaterials meet organ-on-chips – a perspective on tumor modeling DOI
Cátia F. Monteiro, Inês A. Deus, Catarina A. Custódio

et al.

International Materials Reviews, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 10, 2024

Microfluidic organ-on-chip systems are promising platforms for the development of biomimetic models that aim to reconstruct 3D architecture and intrinsic functionality native tissues. An in-depth comprehension pivotal role extracellular matrix in intricate cellular responses has paved way emergence biologically-relevant instructive biomaterials can capture essence cell's microenvironment. The notable evolution realm toward more realistic vitro tissue capable recreating synergistic cell-extracellular interplay is covered. overview most recent advances integrating materials into provided, including exploitation bulk hydrogels as soft material devices fulfill requirements direct cell-matrix interaction. successful application this cutting-edge technology on tumor modeling then discussed, highlighting great contribution perfusable microvessels elucidate mechanistic events metastatic cascade. This convergence science with organ-on-a-chip envisioned foster understanding behavior, shedding light dynamism interactions.

Language: Английский

Citations

0