Effects of Silk Fibroin Hydrogel Degradation on the Proliferation and Chondrogenesis of Encapsulated Stem Cells

Biomacromolecules, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 22, 2025

Silk fibroin (SF) hydrogels are widely used in three-dimensional (3D) cell culture and tissue repair. Despite their importance, few studies have focused on regulating degradation further revealing the effects of process encapsulated behaviors. Herein, SF with equivalent initial properties different rates were prepared by adjusting ratios between hydrogel-encapsulated normal microspheres (MSN) enzyme-loaded (MSE). Further, experiments revealed that moderately accelerating hydrogel obviously improved proliferation MSCs during 7 days culture. Slightly promoted MSC chondrogenesis. However, too rapid a was unfavorable for these The relevant expected to provide useful strategies also afford new references development excellent other protein-based biomaterials cartilage regeneration.

Language: Английский

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Infertility treatment using polysaccharides-based hydrogels: new strategies in tissue engineering and regenerative medicine

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: March 4, 2025

Infertility is a primary health issue affecting about 15% of couples reproductive ages worldwide, leading to physical, mental, and social challenges. Advances in nanobiotechnology regenerative medicine are opening new therapeutic horizons for infertility by developing polysaccharide-based nanostructured biomaterials. This review explores the role tissue engineering treatment, explicitly focusing on promising potential hydrogels. In this context, using these biomaterials offers unique advantages, including biodegradability, biocompatibility, ability mimic natural endometrial microenvironment, making them highly effective applications regeneration, ovarian engineering, spermatogenesis support, controlled drug delivery. discusses various properties uses hydrogels, like alginate, hyaluronic acid, chitosan, helping restore function. While materials hold great promise, some notable challenges their clinical use include issues rapid degradation, mechanical instability, immune reactions. Future research should focus hybrid investigating advanced fabrication techniques, testing settings. By combining findings from recent studies, aims provide solid foundation researchers clinicians looking discover strategies treating infertility, ultimately connecting efforts with practical healthcare.

Language: Английский

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Development and characterization of a novel immortalized human vaginal fibroblast cell line for advanced applications in reproductive health

Reproductive Biology and Endocrinology, Journal Year: 2025, Volume and Issue: 23(1)

Published: April 10, 2025

Reproductive health issues related to the vagina, face significant challenges due lack of standardized research models. Vaginal fibroblasts, which constitute approximately 55% vaginal wall's cellular composition, are crucial for tissue repair, remodelling, and reproductive health. These fibroblasts have broad applications in regenerative medicine gynaecological treatments. Despite their importance, current relies primarily on epithelial cells or primary but limited by short lifespan, donor-to-donor variability, susceptibility senescence. Immortalized fibroblast lines offer a solution extending lifespan enabling reproducible studies. However, well-characterized immortalized human line has not been established, highlighting need novel models better understand address vaginal-associated conditions. Primary were via lentiviral transfection telomerase reverse transcriptase. The resulting cell was characterized through histological, immunofluorescent, RT-qPCR flow cytometry analyses. Proliferation, senescence, gene expression, hormone responsiveness genomic stability assessed quantitative polymerase chain reaction, transcriptome sequencing, set enrichment analysis, tandem repeat profiling, karyotype analysis. (ihVFs) retained typical spindle-shaped morphology fibroblast-specific marker expression. Compared with ihVF exhibited significantly reduced maintained sustained growth extended culture passages, preserved genetic stability. Transcriptome sequencing revealed high expression similarity between no alterations oncogenic pathways. PCR immunofluorescent analyses that ihVFs responsive estrogen progesterone stimulation. Short analysis confirmed novelty line, overlap existing databases. retains key phenotypic, functional, characteristics providing stable, reproducible, physiologically relevant model research. This addresses limitations engineering, disorder research, drug screening, advancing our understanding biology therapeutic interventions.

Language: Английский

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Dual dynamic bonds enable biocompatible polyurethane hydrogels with superior toughness, fatigue and puncture resistance, pH-reversibility, and room-temperature self-healability

Polymer, Journal Year: 2025, Volume and Issue: unknown, P. 128381 - 128381

Published: April 1, 2025

Language: Английский

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3D bioprinted GelMA scaffolds for clinical applications: promise and challenges

Bioprinting, Journal Year: 2024, Volume and Issue: unknown, P. e00365 - e00365

Published: Oct. 1, 2024

Language: Английский

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