
ACS Omega, Journal Year: 2024, Volume and Issue: 9(41), P. 42423 - 42432
Published: Oct. 7, 2024
Spiders can produce up to seven different types of silk, each with unique mechanical properties that stem from variations in the repetitive regions spider silk proteins (spidroins). Artificial be made mini-spidroins an all-aqueous-based spinning process, but strongest fibers seldom reach more than 25% strength native fibers. With aim improve and understand relationship between protein design fibers, we designed 16 new spidroins, ranging 31.7 59.5 kDa, feature globular spidroin N- C-terminal domains, harbor sequences. We found 50% these constructs could spun by extruding them into low-pH aqueous buffer best were produced whose repeat derived major ampullate 4 (MaSp4) elastin. The differed fiber did not correlate expected based on origin repeats, suggesting additional factors beyond impact
Language: Английский