Therapeutic potential of naturally derived carbon dots in sepsis-associated acute kidney injury DOI Creative Commons
Lei Wang,

Zhong-Yao Li,

Chong-Lei Zhong

et al.

Chinese Medicine, Journal Year: 2025, Volume and Issue: 20(1)

Published: April 11, 2025

Abstract Background Sepsis is a life-threatening infectious disease characterized by an uncontrolled inflammatory response and consequent multi-organ dysfunction. The kidneys, as primary excretory organs with high blood flow, are particularly susceptible to damage during sepsis. Nonetheless, the existing treatment options for sepsis-associated acute kidney injury (SA-AKI) still restricted. Nanomedicine, especially carbon dots (CDs), has attracted considerable interest lately outstanding biomedical characteristics. Methods To avoid generation of toxic effects, natural CDs derived from Ziziphi Spinosae Semen (Z-CDs) were synthesized employing hydrothermal method. free radical scavenging capabilities Z-CDs evaluated utilizing ABTS assay, NBT method, Fenton reaction. A lipopolysaccharide (LPS)-stimulated RAW 264.7 cell model was used explore therapeutic potential on cellular oxidative stress inflammation. CuSO 4 -induced zebrafish inflammation LPS-exposed SA-AKI mouse employed assess efficacy in vivo. Results exhibited distinctive unsaturated surface functional groups, which confer exceptional biocompatibility ability scavenge radicals. Moreover, effective eliminating excess reactive oxygen species (ROS) cells, thus protecting mitochondrial function damage. Notably, have demonstrated significant benefits tissue minimizing side effects. In mechanism, effectively reduced ROS production, thereby alleviating responses macrophages through suppression NF-κB pathway. Conclusions This study developed multifunctional nanomedicine traditional medicinal herb, providing promising pathway advancement innovative drug therapies treat SA-AKI.

Language: Английский

Therapeutic potential of naturally derived carbon dots in sepsis-associated acute kidney injury DOI Creative Commons
Lei Wang,

Zhong-Yao Li,

Chong-Lei Zhong

et al.

Chinese Medicine, Journal Year: 2025, Volume and Issue: 20(1)

Published: April 11, 2025

Abstract Background Sepsis is a life-threatening infectious disease characterized by an uncontrolled inflammatory response and consequent multi-organ dysfunction. The kidneys, as primary excretory organs with high blood flow, are particularly susceptible to damage during sepsis. Nonetheless, the existing treatment options for sepsis-associated acute kidney injury (SA-AKI) still restricted. Nanomedicine, especially carbon dots (CDs), has attracted considerable interest lately outstanding biomedical characteristics. Methods To avoid generation of toxic effects, natural CDs derived from Ziziphi Spinosae Semen (Z-CDs) were synthesized employing hydrothermal method. free radical scavenging capabilities Z-CDs evaluated utilizing ABTS assay, NBT method, Fenton reaction. A lipopolysaccharide (LPS)-stimulated RAW 264.7 cell model was used explore therapeutic potential on cellular oxidative stress inflammation. CuSO 4 -induced zebrafish inflammation LPS-exposed SA-AKI mouse employed assess efficacy in vivo. Results exhibited distinctive unsaturated surface functional groups, which confer exceptional biocompatibility ability scavenge radicals. Moreover, effective eliminating excess reactive oxygen species (ROS) cells, thus protecting mitochondrial function damage. Notably, have demonstrated significant benefits tissue minimizing side effects. In mechanism, effectively reduced ROS production, thereby alleviating responses macrophages through suppression NF-κB pathway. Conclusions This study developed multifunctional nanomedicine traditional medicinal herb, providing promising pathway advancement innovative drug therapies treat SA-AKI.

Language: Английский

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