Biomimetic Dynamics of Nanoscale Groove and Ridge Topography for Stem Cell Regulation DOI Creative Commons
Hyunsik Hong, Dahee Kim, Hwapyung Jung

et al.

Advanced Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 26, 2025

Abstract Native extracellular matrix exhibits multiscale groove and ridge structures that continuously change, such as collagen fibril‐based nanogrooves in bone tissue, regulate cellular responses. However, dynamic switching between nanostructures at the molecular level has not been demonstrated. Herein, materials capable of groove‐ridge tens‐of‐nanometers scale are developed by flexibly conjugating RGD‐magnetically activatable nanoridges (MANs) to non‐magnetic with independently tuned widths comparable sizes integrin‐presenting filopodia modulating hydrophobicity bicontinuous microemulsion, allowing for cyclic modulation RGD accessibility adhesion. Nanogrooves medium width restrict “groove” state which RGD‐MANs buried, is reversed magnetically raising them protrude form “ridge” fully exposes RGDs. This reversibly stimulates integrin recruitment, focal adhesion complex assembly, mechanotransduction, differentiation stem cells vivo. first demonstration molecular‐level exhibit unprecedented switchability nanostructures. Versatile tuning width, height, pitch, shape intricate nanogroove remote manipulability can enlighten understanding molecular‐scale cell–ligand interactions cell engineering‐based treatment aging, injuries, stress‐related diseases.

Language: Английский

Biomimetic Dynamics of Nanoscale Groove and Ridge Topography for Stem Cell Regulation DOI Creative Commons
Hyunsik Hong, Dahee Kim, Hwapyung Jung

et al.

Advanced Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 26, 2025

Abstract Native extracellular matrix exhibits multiscale groove and ridge structures that continuously change, such as collagen fibril‐based nanogrooves in bone tissue, regulate cellular responses. However, dynamic switching between nanostructures at the molecular level has not been demonstrated. Herein, materials capable of groove‐ridge tens‐of‐nanometers scale are developed by flexibly conjugating RGD‐magnetically activatable nanoridges (MANs) to non‐magnetic with independently tuned widths comparable sizes integrin‐presenting filopodia modulating hydrophobicity bicontinuous microemulsion, allowing for cyclic modulation RGD accessibility adhesion. Nanogrooves medium width restrict “groove” state which RGD‐MANs buried, is reversed magnetically raising them protrude form “ridge” fully exposes RGDs. This reversibly stimulates integrin recruitment, focal adhesion complex assembly, mechanotransduction, differentiation stem cells vivo. first demonstration molecular‐level exhibit unprecedented switchability nanostructures. Versatile tuning width, height, pitch, shape intricate nanogroove remote manipulability can enlighten understanding molecular‐scale cell–ligand interactions cell engineering‐based treatment aging, injuries, stress‐related diseases.

Language: Английский

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