Biomechanical and mechanobiological design for bioprinting functional microvasculature

Applied Physics Reviews, Journal Year: 2025, Volume and Issue: 12(1)

Published: March 1, 2025

Functional microvasculature is essential for in vitro tissue constructs, ensuring efficient transport of oxygen, nutrients, and waste supporting vital paracrine signaling stability. Recent advancements both direct indirect 3D bioprinting offer promising solutions to construct complex vascular networks by allowing precise control over cell extracellular matrix placement. The process from shape printing function formation involves dynamic shift bioink mechanical properties, microenvironments, mechanobiology endothelial cells. This review explores how biomechanical mechanobiological principles are integrated into the develop functional microvascular networks. Before printing, a top-level design approach based on these focuses interactions among biomaterials, behaviors, environments guide network fabrication. During bioinks different techniques, along with optimized factors process, ensures accurate structure reproduction while maintaining viability. After emphasis creating suitable environment modulate multiple steps neovascularization, including initiation, morphogenesis, lumen formation, stabilization, maturation microvasculature. Finally, we discuss future developments drive functionalized

Language: Английский

---

Engineering in vitro vascular microsystems

Microsystems & Nanoengineering, Journal Year: 2025, Volume and Issue: 11(1)

Published: May 22, 2025

Blood vessels are hierarchical microchannels that transport nutrients and oxygen to different tissues organs, while also eliminating metabolic waste from the body. Disorders of vascular system impact both physiological pathological processes. Conventional animal models complex, high-cost, time-consuming, low-validity, which have limited exploration effective in vitro microsystems. The morphologies micro-scaled tubular structures properties tissues, including mechanical strength, thrombogenicity, immunogenicity, can be mimicked by engineering strategies. This review highlights state-of-the-art advanced strategies for microsystems, covering domains related rational designs, manufacturing approaches, supporting materials, organ-specific cell types. A broad range biomedical applications microsystems summarized, recent advances engineered vascularized organs study, drug screening, personalized medicine. Moreover, commercialization feasibility limitations current commercially available products, as well perspectives on future directions exploration, elaborated. modeling will facilitate rapid, robust, efficient analysis tissue broader regenerative medicine towards development treatment approaches.

Language: Английский

---

Pressure-Driven Perfusion System to Control, Multiplex and Recirculate Cell Culture Medium for Organs-on-Chips

Micromachines, Journal Year: 2022, Volume and Issue: 13(8), P. 1359 - 1359

Published: Aug. 20, 2022

Organ-on-chip (OoC) devices are increasingly used to mimic the tissue microenvironment of cells in intact organs. This includes microchannels mimic, for example, fluidic flow through blood vessels. Present methods controlling microfluidic these systems rely on gravity, rocker or external pressure pumps. For many purposes, pumps give most consistent profiles, but they not well-suited high throughput as might be required testing drug responses. Here, we describe a method which allows multiplexing channels OoC plus accompanying custom software necessary run system. Moreover, show approach is also suitable recirculation culture medium, an essential cost consideration when expensive reagents and “spent” uptake by during transient unidirectional flow.

Language: Английский

---

Multiplexed fluidic circuit board for controlled perfusion of 3D blood vessels-on-a-chip

Lab on a Chip, Journal Year: 2022, Volume and Issue: 23(1), P. 168 - 181

Published: Dec. 9, 2022

We developed a fluidic circuit board for simultaneous perfusion of up to twelve 3D vessels-on-a-chip under comparable wall shear stress using single set control parameters despite high intrinsic sample diameter variation.

Language: Английский

---

Neovascularization of engineered tissues for clinical translation: Where we are, where we should be?

APL Bioengineering, Journal Year: 2021, Volume and Issue: 5(2)

Published: April 5, 2021

One of the key challenges in engineering three-dimensional tissue constructs is development a mature microvascular network capable supplying sufficient oxygen and nutrients to tissue. Recent angiogenic therapeutic strategies have focused on vascularization constructed tissue, its integration

Language: Английский

---