Advanced Materials,
Journal Year:
2025,
Volume and Issue:
unknown
Published: May 13, 2025
Abstract
3D
bioprinting
bridges
tissue
engineering
and
additive
manufacturing,
however
developing
bioinks
with
balanced
biological
physical
properties
remains
a
challenge.
Hyaluronic
acid
(HA)
is
promising
base
material
due
to
its
biocompatibility
cell‐recognition
features.
An
HA‐based
bioink
designed
using
dynamic
disulfide‐crosslinking
at
physiological
pH
by
modifying
HA
cysteine
moieties.
To
overcome
the
slow
gelation
kinetics
typical
of
disulfide‐crosslinked
hydrogels,
potassium
iodide
(KI)
introduced,
accelerating
in
concentration‐dependent
manner.
KI
not
only
enhances
but
also
provides
radical
scavenging
while
maintaining
hydrogel
integrity.
A
low
concentration
(50
m
)
offers
more
than
3
h
printing
window,
ensures
cell
viability,
facilitates
use
fine
needles
(32G,
108
µm
inner
diameter).
This
enables
fabrication
large
(>3
cm)
complex
structures.
Using
this
bioink,
an
osteoarthritis
disease
model
developed
investigate
interactions
between
human
mesenchymal
stromal
cells
(hMSCs)
chondrocytes,
demonstrating
immunomodulatory
effect
hMSCs
on
inflammation‐induced
chondrocytes.
Overall,
addresses
critical
challenges
bioprinting,
providing
robust
platform
for
constructing
innovative
vitro
models
supporting
advancements
modeling
precision
medicine.
Mesenchymal
stem
cells
(MSCs)
play
a
critical
role
in
cell
therapy
due
to
their
tissue-mimicking
abilities.
However,
conventional
2D
culture
conditions
often
lead
the
loss
of
native
hypoxic
niche,
potentially
limiting
therapeutic
efficacy.
3D
bioprinting
offers
method
recreate
intricate
biological
environments
by
integrating
with
extracellular
matrices.
Therefore,
it
is
essential
adapt
printing
techniques
accurately
replicate
MSCs'
ecological
facilitating
integration
technology
into
clinical
applications.
In
this
study,
we
optimized
capabilities
using
performed
cellular
aggregates
(PCA)
method.
We
observed
that
printed
matrix
creates
microenvironment,
resulting
significant
increase
level
production
several
paracrine
signaling
molecules
and
immunomodulatory
factors
MSCs.
Furthermore,
MSCs
exhibited
enhanced
stemness
proliferative
capacity
early
stages
culture.
RNA-seq
analysis
revealed
these
changes
behavior
were
associated
environment
created
during
procedure
By
optimizing
bioink
composition
parameters,
successfully
simulated
vivo
leading
notable
improvements
MSC
characteristics
capacity.
RNA
sequencing
further
confirmed
activation
hypoxia
pathways,
which
are
crucial
for
properties.
These
findings
offer
valuable
insights
leveraging
MSC-based
therapies
regenerative
medicine.
Cells,
Journal Year:
2025,
Volume and Issue:
14(9), P. 669 - 669
Published: May 2, 2025
Osteoarthritis
(OA)
is
a
degenerative
joint
disease
that
significantly
impacts
quality
of
life
and
poses
growing
economic
burden.
Adipose
tissue-derived
therapies,
including
both
cell-based
cell-free
products,
have
shown
promising
potential
in
promoting
cartilage
repair,
modulating
inflammation,
improving
function.
Recent
studies
clinical
trials
demonstrated
their
regenerative
effects,
highlighting
feasibility
as
novel
treatment
approach
for
OA.
This
review
summarises
the
therapeutic
mechanisms
latest
advancements
adipose
providing
insights
into
applications
future
prospects.
Advanced Materials,
Journal Year:
2025,
Volume and Issue:
unknown
Published: May 13, 2025
Abstract
3D
bioprinting
bridges
tissue
engineering
and
additive
manufacturing,
however
developing
bioinks
with
balanced
biological
physical
properties
remains
a
challenge.
Hyaluronic
acid
(HA)
is
promising
base
material
due
to
its
biocompatibility
cell‐recognition
features.
An
HA‐based
bioink
designed
using
dynamic
disulfide‐crosslinking
at
physiological
pH
by
modifying
HA
cysteine
moieties.
To
overcome
the
slow
gelation
kinetics
typical
of
disulfide‐crosslinked
hydrogels,
potassium
iodide
(KI)
introduced,
accelerating
in
concentration‐dependent
manner.
KI
not
only
enhances
but
also
provides
radical
scavenging
while
maintaining
hydrogel
integrity.
A
low
concentration
(50
m
)
offers
more
than
3
h
printing
window,
ensures
cell
viability,
facilitates
use
fine
needles
(32G,
108
µm
inner
diameter).
This
enables
fabrication
large
(>3
cm)
complex
structures.
Using
this
bioink,
an
osteoarthritis
disease
model
developed
investigate
interactions
between
human
mesenchymal
stromal
cells
(hMSCs)
chondrocytes,
demonstrating
immunomodulatory
effect
hMSCs
on
inflammation‐induced
chondrocytes.
Overall,
addresses
critical
challenges
bioprinting,
providing
robust
platform
for
constructing
innovative
vitro
models
supporting
advancements
modeling
precision
medicine.
