Stem cells derived exosomes and biomaterials to modulate autophagy and mend broken hearts DOI Creative Commons

Niketa Sareen,

Abhay Srivastava, Keshav Narayan Alagarsamy

et al.

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Journal Year: 2023, Volume and Issue: 1869(7), P. 166806 - 166806

Published: July 11, 2023

Language: Английский

Functional extracellular vesicles from SHEDs combined with gelatin methacryloyl promote the odontogenic differentiation of DPSCs for pulp regeneration DOI Creative Commons
Hui Lü,

Qing Mu,

Weili Ku

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: May 17, 2024

Abstract Background Pulp regeneration is a novel approach for the treatment of immature permanent teeth with pulp necrosis. This technique includes combination stem cells, scaffolds, and growth factors. Recently, cell-derived extracellular vesicles (EVs) have emerged as new methodology regeneration. Emerging evidence has proven that preconditioning an effective scheme to modify EVs better therapeutic potency. Meanwhile, proper scaffolding great significance protect from rapid clearance destruction. investigation aims fabricate injectable hydrogel loaded pre-differentiated cells human exfoliated deciduous (SHEDs) examine their effects on Results We successfully employed odontogenic induction medium (OM) SHEDs generate functional EV (OM-EV). The OM-EV at concentration 20 µg/mL was demonstrated promote proliferation migration dental (DPSCs). results revealed potential differentiation DPSCs than common (CM-EV) in vitro through Alizarin red phalloidin, alkaline phosphatase staining, assessment expression odontogenic-related markers. High-throughput sequencing suggests superior may be attributed activation AMPK/mTOR pathway. Simultaneously, we prepared photocrosslinkable gelatin methacryloyl (GelMA) construct OM-EV-encapsulated hydrogel. exhibited sustained release good biocompatibility DPSCs. released could internalized by DPSCs, thereby enhancing survival migration. In tooth root slices were subcutaneously transplanted nude mice, found facilitate dentinogenesis. After 8 weeks, there more formation mineralized tissue, well higher levels dentin sialophosphoprotein (DSPP) matrix protein-1 (DMP-1). Conclusions can substantially enhanced SHEDs. combined GelMA are capable effectively promoting dentinogenesis upregulating which provides promising

Language: Английский

Citations

4
Frontier role of extracellular vesicles in kidney disease DOI Creative Commons
Bei Li, Qi Chen, Yifan Zhang

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Sept. 20, 2024

Language: Английский

Citations

4
A review of molecular interplay between inflammation and cancer: The role of lncRNAs in pathogenesis and therapeutic potential DOI

Yan Yang,

Chuxi Zhang,

H C Li

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142824 - 142824

Published: April 1, 2025

Language: Английский

Citations

0
Apoptotic Extracellular Vesicles from Supernumerary Tooth-Derived Pulp Stem Cells Transfer COL1A1 to Promote Angiogenesis via PI3K/Akt/VEGF Pathway DOI Creative Commons
Yue Fei,

Zhichen Ling,

Qian Tong

et al.

International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 6811 - 6828

Published: July 1, 2024

Purpose: Angiogenesis is a tightly controlled process that initiates the formation of new vessels and its dysfunction can lead to life-threatening diseases. Apoptotic extracellular vesicles (ApoEVs) have emerged as proangiogenic agent with high safety isolation efficiency profile, ApoEVs from supernumerary tooth-derived pulp stem cells (SNTSC-ApoEVs) their unique advantages an easily accessible parental cell source non-invasive harvesting. However, detailed characteristics SNTSC-ApoEVs are largely unknown. This study aimed investigate capacity function molecule SNTSC-ApoEVs. Methods: were isolated characterized. In vitro effects on proliferation, migration, tube human umbilical vein endothelial (HUVECs) evaluated by CCK-8, wound healing, transwell, assays. The mRNA protein levels genes quantified qRT-PCR, Western blot, immunofluorescence analysis. A Matrigel plug model was established in 6-week-old male nu/nu mice for one week, vivo impact micro-vessel assessed histological Proteomic analysis RNA sequencing performed explore active ingredients underlying mechanisms. Results: enhanced angiogenesis HUVECs vitro. vivo, promoted CD31-positive luminal structure formation. Apart expressing general ApoEV markers, enriched multiple proteins related matrix-cell interactions. Mechanistically, transferred COL1A1 functions activating PI3K/Akt/VEGF cascade. Conclusion: promote transferring functional pathway, making promising strategy treatment angiogenesis-related Keywords: apoptotic vesicles, cells, angiogenesis, COL1A1, PI3K/Akt pathway

Language: Английский

Citations

3
Stem cells derived exosomes and biomaterials to modulate autophagy and mend broken hearts DOI Creative Commons

Niketa Sareen,

Abhay Srivastava, Keshav Narayan Alagarsamy

et al.

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Journal Year: 2023, Volume and Issue: 1869(7), P. 166806 - 166806

Published: July 11, 2023

Language: Английский

Citations

7