Omnidirectional anisotropic embedded 3D bioprinting DOI Creative Commons
Lei Shao,

Jinhong Jiang,

Chenhui Yuan

et al.

Materials Today Bio, Journal Year: 2024, Volume and Issue: 27, P. 101160 - 101160

Published: July 17, 2024

Anisotropic microstructures resulting from a well-ordered arrangement of filamentous extracellular matrix (ECM) components or cells can be found throughout the human body, including skeletal muscle, corneal stroma, and meniscus, which play crucial role in carrying out specialized physiological functions. At present, due to isotropic characteristics conventional hydrogels, construction freeform cell-laden anisotropic structures with high-bioactive hydrogels is still great challenge. Here, we proposed method for direct embedded 3D cell-printing structure shear-oriented bioink (GelMA/PEO). This study focuses on establishment an bioprinting system, effectively utilizes shear stress generated during extrusion process create encapsulating tissues distinct anisotropy. In conjunction water-solubility PEO in-situ encapsulation effect provided by carrageenan support bath, high-precise intricate porous bionic artificial implemented one-step. Additionally, permeable blood vessel has been taken as representation validate effectiveness system fabricating directional characteristics. Lastly, successful preparation muscle patches properties their guiding cell cytoskeleton extension have significant research foundation application ex-vivo production in-vivo tissues.

Language: Английский

Hierarchical Design of Tissue‐Mimetic Fibrillar Hydrogel Scaffolds DOI Creative Commons
Alberto Pardo, Manuel Gómez‐Florit, Matthew D. Davidson

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(16)

Published: Feb. 24, 2024

Abstract Most tissues of the human body present hierarchical fibrillar extracellular matrices (ECMs) that have a strong influence over their physicochemical properties and biological behavior. Of great interest is introduction this structure to hydrogels, particularly due water‐rich composition, cytocompatibility, tunable class biomaterials. Here, main bottom‐up fabrication strategies for design production biomimetic hydrogels most representative applications in fields tissue engineering regenerative medicine are reviewed. For example, controlled assembly/arrangement peptides, polymeric micelles, cellulose nanoparticles (NPs), magnetically responsive nanostructures, among others, into discussed, as well potential use fibrillar‐like (e.g., those from NPs) with key biofunctionalities such electrical conductivity or remote stimulation. Finally, major remaining barriers clinical translation future directions research field discussed.

Language: Английский

Citations

11
Remendable Cross-Linked Alginate/Gelatin Hydrogels Incorporating Nanofibers for Wound Repair and Regeneration DOI
Changgi Hong,

Haeun Chung,

Gyubok Lee

et al.

Biomacromolecules, Journal Year: 2024, Volume and Issue: 25(7), P. 4344 - 4357

Published: June 25, 2024

Wound dressings made from natural-derived polymers are highly valued for their biocompatibility, biodegradability, and biofunctionality. However, natural polymer-based hydrogels can come with own set of limitations, such as low mechanical strength, limited cell affinity, the potential cytotoxicity cross-linkers, which delineate boundaries usage hamper practical application. To overcome limitation polymers, this study utilized a mixture oxidized alginate gelatin 5 mg/mL polycaprolactone (PCL):gelatin nanofiber fragments at ratio 7:3 (OGN-7) to develop hydrogel composite wound dressing that be injected has ability remended. The in situ formation remendable is facilitated by dual cross-linking chains PCL/gelatin nanofibers through Schiff-base mechanisms, supported physical integration nanofibers, thereby obviating need additional agents. Furthermore, OGN-7 exhibits increased stiffness (γ = 79.4-316.3%), reduced gelation time (543 ± 475 s), improved remendability hydrogel, excellent biocompatibility. Notably, achieves full fusion within 1 h incubation maintains structural integrity under external stress, effectively overcoming inherent weaknesses enhancing therapeutic efficacy was validated full-thickness

Language: Английский

Citations

7
Numerical modeling of hydrogel scaffold anisotropy during extrusion-based 3D printing for tissue engineering DOI
van Than, Robin Chatelin, Edwin‐Joffrey Courtial

et al.

Computer Methods in Biomechanics & Biomedical Engineering, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 20

Published: Jan. 2, 2025

Extrusion-based 3D printing is a widely utilized tool in tissue engineering, offering precise control of bioinks to construct organ-sized biomaterial objects with hierarchically organized cellularized scaffolds. Topological properties flowing polymers are determined by macromolecule conformation, namely orientation and stretch degree. We the micro-macro approach describe hydrogel during extrusion, two-scale fluid behavior description. Results show that shear rate significantly drives alignment, while interaction coefficient (Ci)captures particle interactions. This provides an initial but robust framework model scaffold anisotropy, enabling optimization extrusion process maintaining computational feasibility.

Language: Английский

Citations

0
Enzymatically Degradable Electrospun Nanogel Scaffolds Promoted in Vitro Proliferation of rADSC DOI

Pengwen Li,

Jinze Li,

Hao Zhang

et al.

ACS Applied Polymer Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 10, 2025

Scaffolds that mimic the extracellular matrix (ECM) can promote proliferation of stem cells. Poly(N-isopropylacrylamide) (PNIPAM)/gelatin (PG) nanogel electrospun membranes were designed to replicate hierarchical and dynamic nature ECMs. First, PG nanogels with semi-interpenetrating network structures synthesized by emulsion polymerization. Characterization using 1H NMR FTIR spectroscopies confirmed successful incorporation gelatin into PNIPAM-based network. The spherical a size 184.1–231.9 nm, they demonstrated thermoresponsive behavior. rehydrated nanofibers exhibited an average diameter 663.6 ± 50.1 nm Young's modulus 1.3 0.1 MPa at 37 °C. membrane completely degraded within 10 min collagenase treatment. Adipose-derived cells from rats good on membranes, cell in PNI50 group being 1.47 times tissue culture plate (TCP) group. showed potential for expansion.

Language: Английский

Citations

0
Stimulus-Responsive Cellulose Hydrogels in Biomedical Applications and Challenges DOI Creative Commons

Hui-Fang Xue,

Cong Zhu,

Yifan Wang

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: unknown, P. 101814 - 101814

Published: April 1, 2025

Language: Английский

Citations

0
Current Status of Research on Biomimetic Hydrogels for Articular Cartilage DOI

Kuishun Ma,

Fei Wang,

Tingxin Liang

et al.

Journal of Bionic Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: April 25, 2025

Language: Английский

Citations

0
Improving the quality of steamed rice bread using micro-cellulose as fillers: Effect of particle size DOI
Lin Chen, Qiang Fu, Weiping Jin

et al.

Carbohydrate Polymers, Journal Year: 2025, Volume and Issue: unknown, P. 123663 - 123663

Published: April 1, 2025

Language: Английский

Citations

0
Recent Development of Fibrous Hydrogels: Properties, Applications and Perspectives DOI Creative Commons
Wen Luo,

Liujiao Ren,

Bin Hu

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 12, 2024

Fibrous hydrogels (FGs), characterized by a 3D network structure made from prefabricated fibers, fibrils and polymeric materials, have emerged as significant materials in numerous fields. However, the challenge of balancing mechanical properties functions hinders their further development. This article reviews main advantages FGs, including enhanced properties, high conductivity, antimicrobial anti-inflammatory stimulus responsiveness, an extracellular matrix (ECM)-like structure. It also discusses influence assembly methods, such fiber cross-linking, interfacial treatments fibers with hydrogel matrices, supramolecular assembly, on diverse functionalities FGs. Furthermore, mechanisms for improving performance above five aspects are discussed, creating ion carrier channels situ gelation drugs to enhance antibacterial entanglement hydrophobic interactions between resulting ECM-like structured In addition, this review addresses application FGs sensors, dressings, tissue scaffolds based synergistic effects optimizing performance. Finally, challenges future applications providing theoretical foundation new insights design cutting-edge

Language: Английский

Citations

2
Revisiting gold nanoshells as multifunctional biomedical nanotools DOI Creative Commons
Antonio Topete,

Alejandro Varela,

Mariana Navarro-Real

et al.

Coordination Chemistry Reviews, Journal Year: 2024, Volume and Issue: 523, P. 216250 - 216250

Published: Oct. 11, 2024

Language: Английский

Citations

1
Application Of Tissue Engineering Tools in the treatment of rotator cuff injuries: a bibliometric and visual analysis DOI Creative Commons

Xiangji Meng,

Yuxia Yang,

Jian Zhang

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 19, 2024

Abstract Background Rotator cuff injury is one of the common injuries motor system, which often manifested as shoulder joint pain, limited movement, weakness and even atrophy muscles around joint, accounting for 50% ~ 85% diseases. Tissue engineering uses synthetic or natural biological materials scaffolds, combined with seed cells cytokines to construct implants repair tissue defects, replace rebuild structure organs, maintain improve function tissues organs. In recent years, application tools tendine-bone interface in addressing rotator has emerged a prominent research area. However, no authors have conducted bibliometric visual analysis this field.Methods study, articles related muscular dystrophy omics since 2000 were retrieved from WoSCC, analyzed statistically visually by CiteSpace, VOSViewer R.Results A total 340 papers collected number increasing year year. The published 150 journals 1,588 co-authors 450 institutions 35 countries. 16,116 2,758 cited. Authors US most cited most, other countries also made considerable contributions.Conclusion This first time that results field been visualized through systematic data retrieval variety tools. Through these data, we summarized previous scholars prospected future field.

Language: Английский

Citations

0