Migration Prevention of Carbonate Apatite Granules Through Crystal Interlocking Driven by Bassanite-to-Gypsum Transformation on Granule Surface DOI Creative Commons

L. Escalante,

Koichiro Hayashi, Kunio Ishikawa

et al.

Applied Sciences, Journal Year: 2024, Volume and Issue: 14(23), P. 11261 - 11261

Published: Dec. 3, 2024

Granular bone substitutes are commonly used in dental treatments owing to their adaptability irregular defects. However, granule migration during and after implantation poses a significant challenge, impairing regeneration. This study addresses this issue by setting carbonate apatite (CAp) granules using crystal interlocking the bassanite (calcium sulfate hemihydrate (CSH))-to-gypsum dihydrate (CSD)) transformation on surface. CAp were mixed with CSH slurry (water/CSH ratio of 0.4) at varying CSH/CAp ratios 0.33, 0.43, 0.54, 0.67, 0.82. At all these mixing ratios, needle-shaped CSD crystals formed surface, interlocked each other; consequently, set. As increased from 0.33 0.82, length 6.58 6.79 μm, while time decreased 30.3 15.5 min. Although porosity set an increase ratio, maintained intergranular spaces 77.3 μm 0.82 conducive cellular infiltration. After immersion saline for six days, original shape, demonstrating enhanced stability compared lower where partial or complete collapse occurred. The specific surface area 59.9% 3.66 m2/g, respectively, 176.4 μm. Therefore, may prevent implantation. Moreover, component is likely resorb more rapidly than vivo, promoting facilitating efficient replacement.

Language: Английский

Significance and considerations of establishing standardized critical values for critical size defects in animal models of bone tissue regeneration DOI Creative Commons

Jian Wei,

Xiaohong Chen,

Yingjiao Xu

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(13), P. e33768 - e33768

Published: June 29, 2024

Establishing animal models with critical size defects (CSDs) is for conducting experimental investigations engineering of bone tissue regeneration. Currently, a standardised protocol establishing an CSDs model has not been developed. Furthermore, consensus reached regarding the values CSDs. Successful establishment complex process that requires researchers to meticulously consider variety factors such as age, species, defect and anatomic location. The specific numerical in small vary, clear definition value large literature still lacking. This review consolidates advancements by outlining research landscape across variables, including age groups, sites, sizes, offer valuable guidance theoretical framework pertinent models.

Language: Английский

Citations

8
Carbonate Apatite Honeycomb Scaffold-Based Drug Delivery System for Repairing Osteoporotic Bone Defects DOI Creative Commons
Koichiro Hayashi, Cheng Zhang,

Ahmad Nazir Taleb Alashkar

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(35), P. 45956 - 45968

Published: Aug. 25, 2024

Osteoporotic bone defects are difficult to repair in elderly patients. This study aimed osteoporotic using a combination of tissue engineering (BTE) and drug delivery systems (DDS). Herein, honeycomb granules (HCGs) composed carbonate apatite microspheres were fabricated as BTE scaffolds. Each HCG possesses hexagonal macropores abundant interconnected micropores between the microspheres. Owing these multiscale pores, HCGs can readily contain antibodies against sclerostin (Scl), which causes imbalances homeostasis. Anti-Scl antibody-loaded (Scl-Ab-HCGs) regulate release Scl-Abs response pH environment. In ovariectomized rabbit femurs, monotherapy forms new with less osteocyte damage (fewer empty lacunae) fewer osteoclasts than bone; however, it is insufficient prevent receptor activator nuclear factor-kappa B ligand (RANKL) overexpression. Consequently, restores quantity better no treatment but not normal levels. contrast, formed by Scl-Ab-HCG-based DDS predominantly expresses osteocalcin rather RANKL, similar bone, shows apoptosis level, quantity, osteoclast number bone. Thus, promising approach for defect repair.

Language: Английский

Citations

4
Freeze-drying partially set α-tricalcium phosphate: a new route to granular calcium phosphate cement with enhanced setting ability and porous structure DOI Creative Commons

Abdulrahman Diabi,

Ryo Kishida, Kunio Ishikawa

et al.

Ceramics International, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Low‐crystalline magnesium‐doped carbonate apatite/β‐tricalcium phosphate granules from sea urchin spine DOI Open Access
Muhammad Amir Jamilludin, Koichiro Hayashi, Yusril Yusuf

et al.

Journal of the American Ceramic Society, Journal Year: 2025, Volume and Issue: unknown

Published: March 22, 2025

Abstract The development of synthetic granules with performances equivalent to those autogenous bone grafts is critical for effective replacement. Herein, we report on the fabrication low‐crystalline Mg‐doped biphasic carbonate apatite/β‐tricalcium phosphate (Mg‐CAp/β‐TCP) granules, cylindrical shapes, concave surfaces, and multiscale pores, from sea urchin spines. β‐TCP/CAp ratios Mg contents in Mg‐CAp/β‐TCP were controlled remain within 0.46–0.87 6.03–9.32 mol%, respectively. preserved pores surfaces exhibited inner stereom micropores (3–25 µm), thickened (<14 interseptal gaps (20–43 concavities (26–41 along (<3 µm) nanopores (<100 nm) corresponding intercrystal spaces. rounded uniformly shaped facilitated denser packing a simulated defect mold. compressive diametral tensile strengths 1.12–1.51 0.17–0.21 MPa, distinctive physicochemical structural characteristics can be anticipated facilitate resorption rates promote osteogenesis angiogenesis, facilitating rapid repair. Furthermore, adequate mechanical features enable ease handling during filling potential application load‐bearing sites.

Language: Английский

Citations

0
Development of Zirconia/Calcium Phosphate/Pyrolytic Carbon Composites with Nanoscale Lamellar-Structured Grain Boundary Phases to Control Crack Propagation for Biomedical Applications DOI
Taishi Yokoi, Peng Chen, Kaname Yoshida

et al.

ACS Applied Nano Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

Language: Английский

Citations

0
Fabrication of magnesium-doped biphasic calcium phosphate granules with sea urchin spine-derived porous structure DOI
Muhammad Amir Jamilludin, Koichiro Hayashi, Yusril Yusuf

et al.

Ceramics International, Journal Year: 2024, Volume and Issue: 50(14), P. 25988 - 25999

Published: April 28, 2024

Language: Английский

Citations

3
Lamellar Septa-like Structured Carbonate Apatite Scaffolds with Layer-by-Layer Fracture Behavior for Bone Regeneration DOI Creative Commons

Ahmad Nazir Taleb Alashkar,

Koichiro Hayashi, Kunio Ishikawa

et al.

Biomimetics, Journal Year: 2024, Volume and Issue: 9(2), P. 112 - 112

Published: Feb. 14, 2024

Generally, ceramics are brittle, and porosity is inversely correlated with strength, which one of the challenges ceramic scaffolds. Here, we demonstrate that lamellar septum-like carbonate apatite scaffolds have potential to overcome these challenges. They were fabricated by exploiting cellular structure cuttlebone, removing organic components from performing hydrothermal treatment. Scanning electron microscopy revealed had a walls between septa. The interwall interseptal sizes 80–180 300–500 μm, respectively. size region enclosed septa coincided macropore detected mercury intrusion porosimetry. Although scaffold was extremely high (93.2%), could be handled without disintegration. compressive stress–strain curve demonstrated showed layer-by-layer fracture behavior, seemed beneficial for avoiding catastrophic failure under impact. When implanted into rabbit femurs, new bone blood vessels formed within cells at 4 weeks. At 12 weeks, almost entirely replaced bone. Thus, cellular-structured promising achieving early regeneration compression resistance.

Language: Английский

Citations

2
Synthesis and morphological control of Ca5(PO4)3Cl and Ca2PO4Cl via the phase transformation of amorphous calcium phosphate in molten chlorides DOI

Erlandas Kabasinskas,

Dovydas Karoblis,

Diana Griesiūtė

et al.

Ceramics International, Journal Year: 2024, Volume and Issue: 50(10), P. 16844 - 16851

Published: Feb. 13, 2024

Language: Английский

Citations

1
Hybrid Hydroxyapatite–Metal Complex Materials Derived from Amino Acids and Nucleobases DOI Creative Commons
Alondra Jiménez-Pérez, Marta Martı́nez-Alonso, Javier García‐Tojal

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(18), P. 4479 - 4479

Published: Sept. 20, 2024

Calcium phosphates (CaPs) and their substituted derivatives encompass a large number of compounds with vast presence in nature that have aroused great interest for decades. In particular, hydroxyapatite (HAp, Ca10(OH)2(PO4)6) is the most abundant CaP mineral significant biological world, at least part due to being major compound bones teeth. HAp exhibits excellent properties, such as safety, stability, hardness, biocompatibility, osteoconductivity, among others. Even some its drawbacks, fragility, can be redirected thanks another essential feature: versatility. This based on compound’s tendency undergo substitutions constituent ions incorporate or anchor new molecules surface pores. Thus, affinity biomolecules makes it an optimal multiple applications, mainly, but not only, biomedical fields. The present review provides chemical structural context explain proteins nucleic acids generate hybrid materials. A size-dependent criterium increasing complexity applied, ranging from amino acids/nucleobases corresponding macromolecules. incorporation metal complexes into these functionalized also discussed.

Language: Английский

Citations

0
Migration Prevention of Carbonate Apatite Granules Through Crystal Interlocking Driven by Bassanite-to-Gypsum Transformation on Granule Surface DOI Creative Commons

L. Escalante,

Koichiro Hayashi, Kunio Ishikawa

et al.

Applied Sciences, Journal Year: 2024, Volume and Issue: 14(23), P. 11261 - 11261

Published: Dec. 3, 2024

Granular bone substitutes are commonly used in dental treatments owing to their adaptability irregular defects. However, granule migration during and after implantation poses a significant challenge, impairing regeneration. This study addresses this issue by setting carbonate apatite (CAp) granules using crystal interlocking the bassanite (calcium sulfate hemihydrate (CSH))-to-gypsum dihydrate (CSD)) transformation on surface. CAp were mixed with CSH slurry (water/CSH ratio of 0.4) at varying CSH/CAp ratios 0.33, 0.43, 0.54, 0.67, 0.82. At all these mixing ratios, needle-shaped CSD crystals formed surface, interlocked each other; consequently, set. As increased from 0.33 0.82, length 6.58 6.79 μm, while time decreased 30.3 15.5 min. Although porosity set an increase ratio, maintained intergranular spaces 77.3 μm 0.82 conducive cellular infiltration. After immersion saline for six days, original shape, demonstrating enhanced stability compared lower where partial or complete collapse occurred. The specific surface area 59.9% 3.66 m2/g, respectively, 176.4 μm. Therefore, may prevent implantation. Moreover, component is likely resorb more rapidly than vivo, promoting facilitating efficient replacement.

Language: Английский

Citations

0