Published: Jan. 1, 2024
Language: Английский
Biomacromolecules, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 16, 2025
Traditional polymer systems often rely on toxic initiators or catalysts for cross-linking, posing significant safety risks. For bone tissue engineering, another issue is that the scaffolds take a longer time to degrade, inconsistent with formation pace. Here, we developed an enzyme-responsive biodegradable poly(propylene fumarate) (PPF) and polycaprolactone (PCL) polyphosphoester (PPE) dendrimer cross-linked utilizing click chemistry (EnzDeg-click-PFCLPE scaffold) enhanced biocompatibility degradation. The strain-promoted alkyne–azide cycloaddition (SPAAC) offers high efficiency without harmful agents. polyphosphoesters render cleavage responsive alkaline phosphatase (ALP) enzyme in formation, ensuring facilitated scaffold biodegradation. vitro testing confirmed biocompatibility, degradation, capability support stem cell differentiation. Further vivo implantation rat demonstrated regeneration integration. In summary, this system combining ALP-responsive biodegradation ensures initial facilitates degradation synchronized natural healing process.
Language: Английский
Citations
3
Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown
Published: June 24, 2024
Abstract This study fabricates a functionalized scaffold by cryogenic three‐dimensional (3D) printing using an aminated poly‐L‐lactic acid (EPLA) solution containing nanosilver/zinc‐coated black phosphorus (BP@(Zn+Ag)) nanocomposites. The nanocomposites are prepared green method of in situ photodeposition silver and zinc nanoparticles (AgNPs ZnNPs) on BP nanosheets (BPNs) under visible light irradiation without any chemical reductant. Scanning electron microscope (SEM) X‐ray energy dispersive spectrometer (EDS) confirm the uniform distribution BP@(Zn+Ag) EPLA nanofibrous matrix. vitro tests show that fabricated BP@(Zn+Ag)/EPLA exhibits excellent antibacterial activity (over 96%) against E. coli S. aureus, as well enhanced cell viability osteogenic to facilitate growth differentiation osteoblasts. vivo rat calvarial defect model also demonstrates promotes new bone tissue formation around implant site. Therefore, multifunctional 3D printed has great potential for engineering (BTE) applications.
Language: Английский
Citations
4
Tissue Engineering Part C Methods, Journal Year: 2025, Volume and Issue: unknown
Published: March 10, 2025
Stem cells play a critical role in the regeneration process by proliferating and differentiating to form new bone tissue. However, stem tend lose their stemness pluripotency during vitro expansion, resulting reduced capacity after osteogenic induction. Our aim is enhance impact of human umbilical cord-derived mesenchymal stromal (hUC-MSCs) through spontaneous spheroid vitro. The osteogenesis-related genes up-regulated hUC-MSCs can be enhanced spheroids For vivo testing, were transplanted into mice using beta-tricalcium phosphate as scaffold. Transplant samples stained hematoxylin eosin (HE), immunohistochemistry, TRAP staining. showed formation, upregulated SP7 OCN expression, more vigorous metabolism Sph-OI group than other groups. formation was mainly immature bone. Overall, our findings demonstrate that hUC-MSC possess remarkable pluripotency, with formed following induction exhibiting differentiation potential capacity. optimizing elucidating underlying mechanisms are scientific issues urgently need addressed enable its application regeneration.
Language: Английский
Citations
0
ACS Applied Bio Materials, Journal Year: 2024, Volume and Issue: 7(4), P. 2450 - 2459
Published: March 19, 2024
Spinal injuries or diseases necessitate effective fusion solutions, and common clinical approaches involve autografts, allografts, various bone matrix products, each with limitations. To address these challenges, we developed an innovative moldable click chemistry polymer cement that can be shaped by hand self-cross-linked in situ for spinal fusion. This self-cross-linking cement, enabled the bioorthogonal reaction, excludes need toxic initiators external energy sources. The bioactivity of was promoted incorporating nanohydroxyapatite microspheres loaded recombinant human morphogenetic protein-2 vascular endothelial growth factor, fostering induction osteointegration. release kinetics factors, mechanical properties ability scaffold to support vitro cell proliferation differentiation were evaluated. In a rabbit posterolateral model, exhibited remarkable regeneration bridging spine vertebral bodies. bioactive therefore presents promising biomaterial augmentation, offering advantages safety, ease application, enhanced regrowth.
Language: Английский
Citations
3
Journal of Biomedical Materials Research Part A, Journal Year: 2024, Volume and Issue: 112(10), P. 1803 - 1816
Published: April 21, 2024
Abstract Degenerative spinal pathology is a widespread medical issue, and spine fusion surgeries are frequently performed. In this study, we fabricated an injectable bioactive click chemistry polymer cement for use in bone regrowth. Taking advantages of the bioorthogonal reaction, can be crosslinked by itself eliminating addition toxic initiator or catalyst, nor any external energy sources like UV light heat. Furthermore, nano‐hydroxyapatite (nHA) microspheres carrying recombinant human morphogenetic protein‐2 (rhBMP‐2) vascular endothelial growth factor (rhVEGF) were used to make induction osteointegration. After implantation into rabbit posterolateral (PLF) model, showed excellent new formation bridging bone, achieving results comparable autograft control. This largely due osteogenic properties released rhBMP‐2 rhVEGF factors. Since availability limited clinical settings, may promising alternative applications addressing various conditions.
Language: Английский
Citations
3
Medicina, Journal Year: 2025, Volume and Issue: 61(5), P. 871 - 871
Published: May 9, 2025
Background and Objectives: Dexamethasone has been widely researched for its ability to promote osteogenic differentiation in mesenchymal stem cells basic research. This study focused on examining the effects of dexamethasone both cell viability three-dimensional spheroids. Materials Methods: These spheroids were created using concave microwells exposed at concentrations ranging from 0 μM 100 μM, including intermediate levels 0.1 1 10 μM. Microscopic analysis was used qualitatively assess cellular viability, while a water-soluble tetrazolium salt-based assay provided quantitative data. Osteogenic evaluated by measuring alkaline phosphatase activity calcium deposition Alizarin Red staining. Additionally, expression genes associated with osteogenesis measured through polymerase chain reaction. Results: The successfully self-assembled within first 24 h maintained their structural integrity over seven-day period. Analysis showed no statistically significant differences across various tested. Although there an observed increase following treatment, these not significant. RUNX2 gene upregulated groups, COL1A1 significantly increased Conclusions: results indicate that supports enhances
Language: Английский
Citations
0
Springer eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 2257 - 2307
Published: Jan. 1, 2024
Language: Английский
Citations
1
Springer eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 51
Published: Jan. 1, 2024
Language: Английский
Citations
0
Published: Jan. 1, 2024
Language: Английский
Citations
0