Engineering and Evaluating Vascularized Organotypic Spheroids On‐Chip DOI
James J. Tronolone,

Nadin Mohamed,

Christopher P. Chaftari

et al.

Current Protocols, Journal Year: 2024, Volume and Issue: 4(11)

Published: Nov. 1, 2024

Abstract Organotypic spheroids are evolving as a mainstream in vitro modeling platform, but it is crucial to integrate vascular tissue and perfusion for maintaining their longevity, stability, physiological relevance. Current vascularization methods remain underdeveloped, several protocols poorly reproducible limited use by few select groups who have designed these methods. To achieve standardization, we offer step‐by‐step guide vascularize organotypic case studies of pancreatic islets cancer spheroids. Our systematic approach spans microfluidic chip design, spheroid fabrication, techniques (vasculogenesis angiogenesis) while describing critical engineering We also include additional insights operating guidelines within our that characterize quantitate models with molecular assays well integrated computational algorithms mass transport through formed capillary vessels. These contribute establishing reproducibility, enhanced adoption other contemporary organ‐chip researchers, want engineer vascularized organoid‐based microphysiological platforms. © 2024 Wiley Periodicals LLC. Basic Protocol 1 : Design fabrication chips 2 3 Vascularized on‐chip 4 Functionality Support Cell Culture Immunocytochemistry

Language: Английский

Microgel-based bioink for extrusion-based 3D bioprinting and its applications in tissue engineering DOI

Keerthi Subramanian Iyer,

Lei Bao, Jiali Zhai

et al.

Bioactive Materials, Journal Year: 2025, Volume and Issue: 48, P. 273 - 293

Published: Feb. 20, 2025

Language: Английский

Citations

1
Macroporous Scaffolds Based on Biomass Polymers and Their Applications in Wound Healing DOI Creative Commons
Daqian Gao,

William D. Shipman,

Ya‐Ping Sun

et al.

Journal of Bioresources and Bioproducts, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Language: Английский

Citations

1
Foreign Body Immune Response to Zwitterionic and Hyaluronic Acid Granular Hydrogels Made with Mechanical Fragmentation DOI Creative Commons
Maryam Asadikorayem, Patrick Weber, František Surman

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 5, 2024

Abstract Granular hydrogels have recently attracted the attention for diverse tissue engineering applications due to their versatility and modularity. Despite previous studies showing enhanced viability metabolism of cells encapsulated in these hydrogels, vitro immune response long‐term fibrotic scaffolds not been well characterized. Here, bulk granular are studied based on synthetic zwitterionic (ZI) natural polysaccharide hyaluronic acid (HA) made with mechanical fragmentation. In vitro, immunomodulatory show an increased stimulatory effect HA compared bulk, while both ZI do induce inflammatory response. Subcutaneous implantation mice shows that resulted less collagen capsule deposition around implants 10 weeks after implantation. Moreover, infiltrated by host cells, including macrophages mature blood vessels, a porosity‐dependent manner. However, large number multinucleated giant as surround able infiltrate. Overall, this study provides new insights stability paving way future applications.

Language: Английский

Citations

0
Mechanics-Switchable and Antiswelling Colloidal Hydrogels Inspired by the Cononsolvency Effect DOI
Xuan Xie,

Jingyi Kang,

Si Chen

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(48), P. 66532 - 66542

Published: Nov. 19, 2024

Stiffness switching hydrogels have been in high demand for intelligent devices, adhesion science, soft robotics, and flexible electronics. However, it is challenging to post-tune the mechanical properties of a hydrogel once polymer network structure formed. Inspired by cononsolvency effect, we prepared mechanics-switching colloidal through precipitation polymerization methacrylamide mixed dimethyl sulfoxide water solvents. The enables simultaneously strengthen, stiffen, toughen. not only display broad spectrum adjustable (ranging from 0.08 57.1 MPa) via solvent response but also accomplish reversible stiffness conversion solvent-tuned conformational adjustment networks, with maximum modulus reaching 1427, exhibit solvent-responsive shape memory. Based on stable tight network, antiswelling various aqueous solutions would provide more possibilities applications, such as antiimpact protection, memory, electronic switches, engineering materials. It envisioned that this work will key opportunities developing on-demand tuning materials tissue engineering, electronics, biomedical science.

Language: Английский

Citations

0
Engineering and Evaluating Vascularized Organotypic Spheroids On‐Chip DOI
James J. Tronolone,

Nadin Mohamed,

Christopher P. Chaftari

et al.

Current Protocols, Journal Year: 2024, Volume and Issue: 4(11)

Published: Nov. 1, 2024

Abstract Organotypic spheroids are evolving as a mainstream in vitro modeling platform, but it is crucial to integrate vascular tissue and perfusion for maintaining their longevity, stability, physiological relevance. Current vascularization methods remain underdeveloped, several protocols poorly reproducible limited use by few select groups who have designed these methods. To achieve standardization, we offer step‐by‐step guide vascularize organotypic case studies of pancreatic islets cancer spheroids. Our systematic approach spans microfluidic chip design, spheroid fabrication, techniques (vasculogenesis angiogenesis) while describing critical engineering We also include additional insights operating guidelines within our that characterize quantitate models with molecular assays well integrated computational algorithms mass transport through formed capillary vessels. These contribute establishing reproducibility, enhanced adoption other contemporary organ‐chip researchers, want engineer vascularized organoid‐based microphysiological platforms. © 2024 Wiley Periodicals LLC. Basic Protocol 1 : Design fabrication chips 2 3 Vascularized on‐chip 4 Functionality Support Cell Culture Immunocytochemistry

Language: Английский

Citations

0