Expanding the capillarics toolbox: 3D-printed microfluidic phaseguides and self-coalescence modules DOI Creative Commons
Cosette Craig, Kelsey Leong, Carrie H. Lin

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 22, 2024

Abstract Capillarics are microfluidic circuits that assembled from individual fluidic elements, powered by surface tension forces encoded microchannel geometry and chemistry, enable instrument-free pre-programmed automation of multi-step liquid handling processes. 3D printing has recently transformed capillarics enabling rapid cost-effective prototyping, provided addition geometric degrees freedom in multi-level fabrication, facilitated new design paradigms with greater capabilities than traditional cleanroom fabrication. Despite widespread interest development custom high-resolution stereolithography printers for applications, elements require precise tunable control over capillary pinning lines – such as phaseguides self-coalescence modules (SCMs) have so far only been manufactured centralized expensive methods. Not does this limit access to versatile capillaric features well-resourced settings, but it also slows innovation application these fluid technologies. Here we expand the toolbox 3D-printed include SCMs, demonstrating their potential reagent rehydration. We employed benchtop prototype (up 50X) scaled-up SCMs integrated them into a circuit inline reconstitution, dynamic control, sequential drainage. showcased scalable designs, customizable geometries, robust self-coalescing flow larger volumes up 50 µL compared 1.25 cleanroom-fabricated SCMs. This work represents significant advance democratizing microfluidics, broad applications diagnostics, assay automation, organ-on-chip systems.

Language: Английский

Latest developments of microphysiological systems (MPS) in aging-related and geriatric diseases research: a review DOI
Chao Song, Jiachen Yang,

Zhongze Gu

et al.

Ageing Research Reviews, Journal Year: 2025, Volume and Issue: unknown, P. 102728 - 102728

Published: March 1, 2025

Language: Английский

Citations

0
Fibrosis Drug Efficacy Assessment Based on Microfluidic Mechanical Property Evaluation of Spheroid Models DOI Open Access

B.H. Kim,

Jeong Yeon Kim, Hye Won Kim

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 16, 2025

Abstract Fibrotic diseases, such as pulmonary fibrosis, pose significant challenges in both research and treatment. To address the limitations of existing systems, a novel collision‐based spheroid mechanical property assessment system is developed. The utilizes inertial fluid dynamics to induce controlled collisions through uniformly sized spheroids, allowing strain be measured via high‐speed cameras. In this study, first validated using HEK293T spheroids optimize flow velocity, followed by an analysis deformability differences between two cell types related fibrosis (Calu‐1 MRC‐5). A co‐culture model comprising lung cells, endothelial fibroblast different rations further developed, depending on composition observed. Finally, are treated with TGF‐β1(Transforming Growth Factor‐β1), factor known activate cells excessive extra cellular matrix (ECM) accumulation, Nintedanib, anti‐fibrotic drug, assess changes properties. These results effectively reflect properties driven cell‐cell cell‐ECM interactions highlight correlation mechanics progression fibrotic disease. This not only contributes deeper understanding but also serves powerful platform for accelerating development therapies.

Language: Английский

Citations

0
Expanding the capillarics toolbox: 3D-printed microfluidic phaseguides and self-coalescence modules DOI Creative Commons
Cosette Craig, Kelsey Leong, Carrie H. Lin

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 22, 2024

Abstract Capillarics are microfluidic circuits that assembled from individual fluidic elements, powered by surface tension forces encoded microchannel geometry and chemistry, enable instrument-free pre-programmed automation of multi-step liquid handling processes. 3D printing has recently transformed capillarics enabling rapid cost-effective prototyping, provided addition geometric degrees freedom in multi-level fabrication, facilitated new design paradigms with greater capabilities than traditional cleanroom fabrication. Despite widespread interest development custom high-resolution stereolithography printers for applications, elements require precise tunable control over capillary pinning lines – such as phaseguides self-coalescence modules (SCMs) have so far only been manufactured centralized expensive methods. Not does this limit access to versatile capillaric features well-resourced settings, but it also slows innovation application these fluid technologies. Here we expand the toolbox 3D-printed include SCMs, demonstrating their potential reagent rehydration. We employed benchtop prototype (up 50X) scaled-up SCMs integrated them into a circuit inline reconstitution, dynamic control, sequential drainage. showcased scalable designs, customizable geometries, robust self-coalescing flow larger volumes up 50 µL compared 1.25 cleanroom-fabricated SCMs. This work represents significant advance democratizing microfluidics, broad applications diagnostics, assay automation, organ-on-chip systems.

Language: Английский

Citations

0