Human umbilical cord mesenchymal stem cells restores mTOR-mediated autophagy homeostasis to alleviate placental injury and improve pregnancy outcomes in preeclampsia DOI
Miao Xu, Huijing Ma, Yu‐Wen Chen

et al.

Published: Oct. 23, 2024

Abstract Background Preeclampsia is a hypertensive disorder during pregnancy, which seriously threatens both maternal and infant health. Currently, the only treatment available to induce placenta delivery, resulting in interest potential fetal-safe strategies. One such strategy cell therapy with human umbilical cord mesenchymal stem cells (hUC-MSCs), possesses immunomodulatory, anti-inflammatory angiogenic functions that could alleviate pre-eclamptic symptoms. However, precise effects underlying mechanisms behind their activities are still largely unknown. In this study, we aimed elucidate effect of hUC-MSCs, as well pathways involved, on placental function preeclampsia, thereby highlighting novel avenue for therapy. Methods Both an in vivo rat model, involving N-nitro-L-arginine methyl ester (L-NAME) injections pregnant rats, vitro entailing HTR8 trophoblasts/human vein endothelial (HUVECs) being stimulated lipopolysaccharide (LPS), were established simulate pre-eclampsia. In vivo, blood pressure, renal function, fetal weights, measured. ELISA was used measure serum levels angiogenic, inflammatory, oxidative stress factors. Placental mitochondrial morphology evaluated using transmission electron microscopy, while autophagic analyzed by Western blots. With proliferation, invasion, stress, apoptosis Transwell co-cultured hUC-MSCs. Results hUC-MSC administration found model increase along alleviating hypertension proteinuria, owed those promoting angiogenesis perfusion, lowering inflammation, apoptosis. These findings further supported where co-culture LPS-treated HTR8/HUVECs resulted increased proliferation lowered reactive oxygen species generation. All these hUC-MSCs improving autophagy; through activating Akt/mTOR inhibiting AMPK/mTOR pathways, leading pro-autophagic LC3 Beclin1 downregulation, anti-autophagic P62 upregulation. Conclusion able pre-eclampsia restoring physiological homeostasis, serve promising therapeutic disease.

Language: Английский

Trends in sustainable chitosan-based hydrogel technology for circular biomedical engineering: A review DOI
Anshuman Mishra,

Temitayo Margaret Omoyeni,

Pravin Kumar Singh

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 276, P. 133823 - 133823

Published: July 11, 2024

Language: Английский

Citations

26
Hydrogel-exosome system in tissue engineering: A promising therapeutic strategy DOI Creative Commons

Ming‐Hui Fan,

Jin-Kui Pi,

Chen‐Yu Zou

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 38, P. 1 - 30

Published: April 23, 2024

Characterized by their pivotal roles in cell-to-cell communication, cell proliferation, and immune regulation during tissue repair, exosomes have emerged as a promising avenue for "cell-free therapy" clinical applications. Hydrogels, possessing commendable biocompatibility, degradability, adjustability, physical properties akin to biological tissues, also found extensive utility engineering regenerative repair. The synergistic combination of hydrogels holds the potential not only enhance efficiency but collaboratively advance repair process. This review has summarized advancements made over past decade research hydrogel-exosome systems regenerating various tissues including skin, bone, cartilage, nerves tendons, with focus on methods encapsulating releasing within hydrogels. It critically examined gaps limitations current research, whilst proposed future directions applications this innovative approach.

Language: Английский

Citations

21
A Novel Conductive Polypyrrole‐Chitosan Hydrogel Containing Human Endometrial Mesenchymal Stem Cell‐Derived Exosomes Facilitated Sustained Release for Cardiac Repair DOI Creative Commons
Changping Yan, Xinzhu Wang, Qi Wang

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(10)

Published: Jan. 4, 2024

Abstract Myocardial infarction (MI) results in cardiomyocyte necrosis and conductive system damage, leading to sudden cardiac death heart failure. Studies have shown that biomaterials can restore conduction, but cannot facilitate tissue regeneration. This study aims add regenerative capabilities the biomaterial by incorporating human endometrial mesenchymal stem cell (hEMSC)‐derived exosomes (hEMSC‐Exo) into poly‐pyrrole‐chitosan (PPY‐CHI), yield an injectable hydrogel effectively treat MI. In vitro, PPY‐CHI/hEMSC‐Exo, compared untreated controls, PPY‐CHI, or hEMSC‐Exo alone, alleviates H 2 O ‐induced apoptosis promotes tubule formation, while vivo, PPY‐CHI/hEMSC‐Exo improves post‐MI functioning, along with counteracting against ventricular remodeling fibrosis. All these activities are facilitated via increased epidermal growth factor (EGF)/phosphoinositide 3‐kinase (PI3K)/AKT signaling. Furthermore, properties of able resynchronize electrical transmission alleviate arrythmia. Overall, synergistically combines PPY‐CHI improve promoting angiogenesis inhibiting apoptosis, as well resynchronizing ultimately enable more effective MI treatment. Therefore, a provides dual benefits terms maintaining conductivity, facilitating long‐term exosome release sustained application their beneficial effects.

Language: Английский

Citations

18
A novel exosome-based multifunctional nanocomposite platform driven by photothermal-controlled release system for repair of skin injury DOI
Xu Teng, Tao Liu, Guifang Zhao

et al.

Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 371, P. 258 - 272

Published: May 31, 2024

Language: Английский

Citations

8
Unlocking the Potential of Extracellular Vesicles in Cardiovascular Disease DOI Creative Commons
Hanbin Li, Lu Wang, Hongxin Cheng

et al.

Journal of Cellular and Molecular Medicine, Journal Year: 2025, Volume and Issue: 29(3)

Published: Feb. 1, 2025

ABSTRACT Extracellular vesicles (EVs) are micro‐nanoscale biological particles encapsulated by phospholipid bilayers, which regulate cell migration, angiogenesis and tumour growth transmitting various biomolecules such as nucleic acids proteins. EVs composed of exosomes, microparticles apoptotic bodies. Its benefits pass through biofilms not degraded enzymes, so it can be used a biomarker in potential diseases has attracted much attention from researchers. Current studies have found that involved the development cardiovascular (CVD), heart failure myocardial ischemia–reperfusion injury. In addition, stem cell‐derived play an important role diagnosis treatment variety CVD. this review, we present features EVs, CVD, challenges they encounter

Language: Английский

Citations

1
Unpacking Exosomes: A Therapeutic Frontier for Cardiac Repair DOI Creative Commons

Elena McMullan,

Darukeshwara Joladarashi,

Raj Kishore

et al.

Current Cardiology Reports, Journal Year: 2025, Volume and Issue: 27(1)

Published: March 20, 2025

Abstract Purpose of Review The rising global prevalence cardiovascular disease is driving the need for innovative biotherapeutics. Recently, exosomes-extracellular vesicles involved in paracrine signaling have shown promise aiding heart repair associated with conditions. Their therapeutic potential encompasses several beneficial mechanisms, including anti-fibrosis, anti-inflammation, pro-angiogenesis, anti-oxidation, and anti-apoptosis, all contributing to improved cardiac function. This review provides a comprehensive overview exosomes highlights latest research on their effectiveness addressing current challenges regenerative medicine. Recent Findings Current approaches revolve around elucidating enhancing how different cell types, cargo, delivery methods impact healing pathological environment. Summary emerging field exosome promising regeneration due effects exosomal cargo. expansion mechanistic knowledge optimization techniques are required before standard clinical application.

Language: Английский

Citations

1
Therapeutic nucleic acids in regenerative medicine and tissue repair DOI
Qimanguli Saiding, Duotian Qin, Soohwan An

et al.

Nano Research, Journal Year: 2024, Volume and Issue: 17(10), P. 8942 - 8976

Published: July 13, 2024

Language: Английский

Citations

4
Novel Electroactive Therapeutic Platforms for Cardiac Arrhythmia Management DOI Creative Commons
J. C. Yang, Longfei Li, Yiran Hu

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 14, 2025

Abstract Electroactive platforms have gained significant attention for their ability to convert various types of energy into electrical signals, offering promising applications in diverse biomedical fields. In cardiovascular care, these are increasingly valued innovative solutions managing cardiac functions and disorders. By regulating activity the heart, electroactive offer novel methods abnormal heart rhythms. This review explores latest advancements systems, categorizing them based on sources mechanisms, such as self‐powered conductive systems. It also highlights arrhythmia management, including monitoring, intervention, pacing, repairing. Finally, challenges, limitations, future opportunities clinical translation technologies discussed.

Language: Английский

Citations

0
Engineering functional electroconductive hydrogels for targeted therapy in myocardial infarction repair DOI

Qiang Lv,

Dandan Zhou, Yutong He

et al.

Bioactive Materials, Journal Year: 2025, Volume and Issue: 49, P. 172 - 192

Published: March 9, 2025

Language: Английский

Citations

0
Hydrogels for Cardio and Vascular Tissue Repair and Regeneration DOI Creative Commons
Ilenia Motta, Michelina Soccio, Giulia Guidotti

et al.

Gels, Journal Year: 2024, Volume and Issue: 10(3), P. 196 - 196

Published: March 13, 2024

Cardiovascular disease (CVD), the leading cause of death globally, affects heart and arteries with a variety clinical manifestations, most dramatic which are myocardial infarction (MI), abdominal aortic aneurysm (AAA), intracranial (IA) rupture. In MI, necrosis myocardium, scar formation, loss cardiomyocytes result from insufficient blood supply due to coronary artery occlusion. Beyond stenosis, that structurally functionally connected cardiac tissue can undergo pathological dilation, i.e., aneurysmal high risk Aneurysms (IAs) more commonly seen in young adults, whereas those aorta (AAA) predominantly elderly. IAs, unpredictably, rupture life-threatening hemorrhage, while AAAs rupture, internal bleeding mortality rate. this context, hydrogels, three-dimensional networks water-seizing polymers, have emerged as promising biomaterials for cardiovascular repair or protection their biocompatibility, tunable properties, ability encapsulate release bioactive molecules. This review provides an overview current state research on use hydrogels innovative platform promote cardiovascular-specific MI functional recovery dilation.

Language: Английский

Citations

3