Poly-Adenine Assisted Signaling Displaced Probe Ratiometric Electrochemical Aptasensor for Accurate Detection of Alzheimer’s Disease Aβ Biomarkers DOI
Jiajun Tong, Chengxiang Li, J. Zhao

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(46), P. 64297 - 64306

Published: Nov. 8, 2024

Alzheimer's disease (AD) is a widely prevalent neurodegenerative condition globally, arousing significant interest in the noninvasive early detection of disease. The concentration amyloid β (Aβ) biomarkers blood closely linked to progression AD, emphasizing importance developing precise method for detecting these samples diagnosis. In this study, we developed ratiometric electrochemical aptamer-based (EAB) biosensor accurate Aβ42 and Aβ40. utilizes gold nanoparticle (AuNPs) modified electrode through an deposition assay self-assembled poly-A based U-shaped structure achieve signal changes competitive binding target molecules. Importantly, using calibration clipping reference probe, proposed displayed ability Aβ40, with limits 59 21 pM, respectively. It also has great stability anti-interference complex biological samples. Furthermore, it demonstrated satisfactory performance Aβ peptides 20% FBS sample, recoveries ranging from 96.7% 102.2% serum samples, relative standard deviations (RSD) 3.3% 8.9%. Moreover, expected directly quantify Aβ42/Aβ40 ratio by introducing suitable providing potential effective tool diagnosis AD.

Language: Английский

Freestanding Nanofiber‐Assembled Aptasensor for Precisely and Ultrafast Electrochemical Detection of Alzheimer's Disease Biomarkers DOI Creative Commons
Hui Liu,

Xueli Yuan,

Tao Liu

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(15)

Published: Feb. 22, 2024

Abstract Amyloid beta‐protein (AβA β ) is a main hallmark of Alzheimer's disease (AD), and low amount Aβ protein accumulation appears to be potential marker for AD. Here, an electrochemical DNA biosensor based on polyamide/polyaniline carbon nanotubes (PA/PANI‐CNTs) developed with the aim diagnosing AD early using simple, low‐cost, accessible method rapidly detect Aβ42 in human blood. Electrospun PA nanofibers served as skeleton successive situ deposition PANI CNTs, which contribute both high conductivity abundant binding sites aptamers. After aptamers are immobilized, this aptasensor exhibits precise specific detection blood within only 4 min extremely fast response rate, lower limit, excellent linear range. These findings make significant contribution advancing development serum‐based techniques Aβ42, thereby paving way improved diagnostic capabilities field

Language: Английский

Citations

10
A colorimetric nano-enzyme assay with Ni@Pt nanoparticles as signal labels for rapid and sensitive detection of exosomal Aβ42 in plasma DOI
Junli Zhang, Yanyan Zhao, Haiying Hu

et al.

Microchimica Acta, Journal Year: 2025, Volume and Issue: 192(1)

Published: Jan. 1, 2025

Language: Английский

Citations

1
Electrochemical biosensors for early detection of Alzheimer’s disease DOI

Sanoeva Matlyuba Jakhonkulovna,

Gulnoz Bahodirova Kamolovna,

Muzaffar Zokirov

et al.

Clinica Chimica Acta, Journal Year: 2025, Volume and Issue: unknown, P. 120278 - 120278

Published: April 1, 2025

Language: Английский

Citations

0
Engineered Intelligent Electrochemical Biosensors for Portable Point-of-Care Diagnostics DOI Creative Commons

Jiamin Lin,

Yuanyuan Chen, Xiaohui Liu

et al.

Chemosensors, Journal Year: 2025, Volume and Issue: 13(4), P. 146 - 146

Published: April 16, 2025

The development of cost-effective, rapid-response, and user-friendly biosensing platforms has become paramount importance for achieving precise biomarker quantification in early disease detection. Implementing timely diagnostic interventions through accurate analysis not only significantly improves treatment outcomes but also enables effective management strategies, ultimately leading to substantial reductions patient mortality rates. These clinical imperatives have consequently driven the innovation portable point-of-care (POC) systems. Electrochemical biosensors are attractive diagnosis diseases due their low cost, simple operation, high sensitivity. This review examines prevalent material innovations electrode functionalization electrochemical platforms, with specific emphasis on translational applications early-stage included three important types: proteins, nucleic acids, small molecule metabolites. Furthermore, work proposes novel research trajectories next-generation biosensor development, advocating synergistic integration artificial intelligence-driven analytics, Internet Medical Things (IoMT)-enabled networks, advanced micro/nanofabrication techniques.

Language: Английский

Citations

0
Electrospinning and electrospun nanofibers: From academic research to industrial production DOI
Ce Wang, Wei Wang,

Hongxu Qi

et al.

Progress in Materials Science, Journal Year: 2025, Volume and Issue: unknown, P. 101494 - 101494

Published: April 1, 2025

Language: Английский

Citations

0
Current progress in aptamer-based sensors for the detection of protein biomarkers in neurodegenerative diseases DOI Creative Commons
Tao Cheng, Noshin Afshan, Jianwei Jiao

et al.

Biosensors and Bioelectronics X, Journal Year: 2024, Volume and Issue: 20, P. 100528 - 100528

Published: Aug. 8, 2024

Neurodegenerative diseases (NDs) have been a group of disorders characterized by neuronal death and functional loss including Alzheimer's disease (AD), Parkinson's (PD) Huntington's (HD), etc. Since diagnostic methods for the NDs biomarkers based on traditional enzyme linked immunosorbent assay (ELISA) or Western Blotting, antibody intrinsic drawbacks such as large molecular weight high cost make early diagnosis quite challenging. The aptamers bridge this gap effectively their versatile features small weight, easy modification, stability low widely used to fabricate biosensors diagnose NDs. Various aptamer-based shown excellent performance in Several nucleic acid, protein, biomolecule, exosome has indicators In minireview, we aim report latest progress aptasensors (including colorimetric, fluorescence, electrochemical electrochemiluminescence, etc) detecting protein biomarkers, summarize principles functions along with challenges way emphasize future perspectives. • Review neurodegenerative Discuss advantages disadvantages Limitations conventional were also discussed. Point out gaps aptamer guided research direction

Language: Английский

Citations

2
Recent Advances in Polymer-based Thin-film Electrode for ECoG Applications DOI Creative Commons

Zhengchen Xiang,

Liangtao Yang,

Bin Yu

et al.

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 12, 2024

This review evaluated electrocorticography (ECoG) electrode performance based on key properties and improvements from polymers, categorizing them as sensitive or structural, while discussing preparation methods, challenges, future perspectives.

Language: Английский

Citations

2
Electrochemical detection of tumor cells based on proximity labelling-assisted multiple signal amplification DOI Creative Commons

Guozhang Zhou,

Fei Zhou,

Xiaomeng Yu

et al.

Sensors & Diagnostics, Journal Year: 2024, Volume and Issue: 3(9), P. 1542 - 1550

Published: Jan. 1, 2024

An electrochemical sensing method is developed based on proximity labeling-assisted multiple signal amplification technique. This enables highly sensitive, selective and stable detection of MUC-1 positive tumor cells.

Language: Английский

Citations

0
In situ construction of oriented Au/PPy nanorod arrays-based aptasensor for fast and ultrasensitive determination of thrombin DOI

Xueli Yuan,

Yu Liu,

Shanghui Hu

et al.

Microchemical Journal, Journal Year: 2024, Volume and Issue: 207, P. 112053 - 112053

Published: Nov. 1, 2024

Language: Английский

Citations

0
Poly-Adenine Assisted Signaling Displaced Probe Ratiometric Electrochemical Aptasensor for Accurate Detection of Alzheimer’s Disease Aβ Biomarkers DOI
Jiajun Tong, Chengxiang Li, J. Zhao

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(46), P. 64297 - 64306

Published: Nov. 8, 2024

Alzheimer's disease (AD) is a widely prevalent neurodegenerative condition globally, arousing significant interest in the noninvasive early detection of disease. The concentration amyloid β (Aβ) biomarkers blood closely linked to progression AD, emphasizing importance developing precise method for detecting these samples diagnosis. In this study, we developed ratiometric electrochemical aptamer-based (EAB) biosensor accurate Aβ42 and Aβ40. utilizes gold nanoparticle (AuNPs) modified electrode through an deposition assay self-assembled poly-A based U-shaped structure achieve signal changes competitive binding target molecules. Importantly, using calibration clipping reference probe, proposed displayed ability Aβ40, with limits 59 21 pM, respectively. It also has great stability anti-interference complex biological samples. Furthermore, it demonstrated satisfactory performance Aβ peptides 20% FBS sample, recoveries ranging from 96.7% 102.2% serum samples, relative standard deviations (RSD) 3.3% 8.9%. Moreover, expected directly quantify Aβ42/Aβ40 ratio by introducing suitable providing potential effective tool diagnosis AD.

Language: Английский

Citations

0