Thermoforming for Small Feature Replication in Melt Electrowritten Membranes to Model Kidney Proximal Tubule DOI Creative Commons
Marta G. Valverde,

Claudia Stampa Zamorano,

Dora Kožinec

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 7, 2024

Abstract A novel approach merging melt electrowriting (MEW) with matched die thermoforming to achieve scaffolds micron‐sized curvatures (200 – 800 µm versus 1000 of mandrel printing) for in vitro modeling the kidney proximal tubule (PT) is proposed. Recent advances this field emphasize relevance accurately replicating intricate tissue microenvironment, particularly curvature nephrons’ tubular segments. While MEW offers promising capabilities fabricating highly and porous precise 3D structures mimicking PT, challenges persist approximating diameter match actual PT. The thermoformed membranes retain initial printing design parameters (rhombus geometry, porosity > 45%) while following imprinted (ratios between 0.67‐0.95). PT epithelial cells cultured on these demonstrate ability fill large pores membrane by secreting their own collagen IV‐rich extracellular matrix form an organized, functional, tight monolayer expressing characteristic markers. Besides architecture, setup maximizes usable surface area cell culture molecular readouts. By closely structural intricacies native enhances biomimetic fidelity engineered scaffolds, offering potential applications beyond engineering.

Language: Английский

Thermoforming for Small Feature Replication in Melt Electrowritten Membranes to Model Kidney Proximal Tubule DOI Creative Commons
Marta G. Valverde,

Claudia Stampa Zamorano,

Dora Kožinec

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 7, 2024

Abstract A novel approach merging melt electrowriting (MEW) with matched die thermoforming to achieve scaffolds micron‐sized curvatures (200 – 800 µm versus 1000 of mandrel printing) for in vitro modeling the kidney proximal tubule (PT) is proposed. Recent advances this field emphasize relevance accurately replicating intricate tissue microenvironment, particularly curvature nephrons’ tubular segments. While MEW offers promising capabilities fabricating highly and porous precise 3D structures mimicking PT, challenges persist approximating diameter match actual PT. The thermoformed membranes retain initial printing design parameters (rhombus geometry, porosity > 45%) while following imprinted (ratios between 0.67‐0.95). PT epithelial cells cultured on these demonstrate ability fill large pores membrane by secreting their own collagen IV‐rich extracellular matrix form an organized, functional, tight monolayer expressing characteristic markers. Besides architecture, setup maximizes usable surface area cell culture molecular readouts. By closely structural intricacies native enhances biomimetic fidelity engineered scaffolds, offering potential applications beyond engineering.

Language: Английский

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