Hierarchical Biomimetic Electrospun Vascular Grafts for Improved Patency and Regeneration

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 3, 2025

Abstract Small‐diameter vascular grafts (SDVGs) are essential for cardiovascular disease treatment, particularly in arterial replacement and bypass surgeries. Despite their importance, SDVGs often fail due to thrombosis, inflammation, intimal hyperplasia, limiting clinical application. This study develops a hierarchical biomimetic SDVG with bilayered structure, comprising dense inner layer loose outer larger pores, integrated multifunctional coating containing polydopamine (PDA), copper ions (Cu 2 ⁺), REDV peptides. The PDA‐based hydrophilic mimics the endothelial glycocalyx, reducing platelet adhesion, suppressing macrophage activation, promoting M2 polarization. These effects, along Cu‐catalyzed nitric oxide (NO) release, regulate thrombosis establishing favorable microenvironment healing regeneration. peptides synergize NO selectively enhance cell (EC) proliferation, migration, enabling rapid endothelialization. porous facilitates smooth muscle (SMC) infiltration, while inhibit excessive SMC preventing restenosis. In vitro vivo studies, including implantation Beagle dog iliac arteries, demonstrate that graft achieves long‐term patency, superior hemocompatibility, balanced tissue work offers promising strategy overcome current limitations SDVGs, paving way advanced repair

Language: Английский

Amino-Functionalized Mesoporous Silica Film as a Spatiotemporally Matched Degradable Nanotopography to Enhance Early Bioactivity and Osteogenesis on Titania Nanotube Surfaces

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: March 31, 2025

Nanotopographic fabrication has been proven to enhance the osteoinductivity of titanium implant surfaces; however, it is difficult for static nanostructures regulate multiple osteoblast behaviors. Herein, we proposed a novel strategy further modifying nanostructured surfaces using degradable nanotopography that was beneficial specific cellular processes and spatiotemporally matched. In this work, titania nanotube (TNT) array, known its strong capability promote osteogenic differentiation, employed as substrate. An oil-water biphase system containing 3-aminopropyl triethoxysilane (APTES) tetraethyl orthosilicate (TEOS) utilized achieve in situ deposition amino-functionalized mesoporous silica films on TNT surface (TNT@AHMS). The numerous mesopores (∼4 nm) amino groups AHMS significantly improved protein adsorption attachment rat bone marrow mesenchymal stem cells (rBMSCs). By culturing an AHMS-conditioned medium, effects enhancing early cell behavior were observed initially attributed potential synergism topography silicon element release (∼18 ppm). Impressively, effect maintained even when reseeded normal culture substrates. After 24 h, degraded completely, degradation products facilitated subsequent re-exposed TNT, which accounted robust osteogenesis both vitro vivo. This study demonstrated can serve (like buffer layer) accelerate adhesion matched manner, resulting enhanced bioactivity well-designed underlying without influencing physicochemical properties.

Language: Английский