Identification of biomarkers related to autophagy in osteoarthritis via bioinformatics analysis and experimental validation (Preprint) DOI

Enhong Song,

Gang‐Ming Zou, Chong Shen

et al.

Published: Nov. 7, 2024

BACKGROUND Osteoarthritis(OA) is a degenerative disease with few effective early interventions and therapeutic options, autophagy thought to play significant role in progression of OA. OBJECTIVE We used bioinformatics analysis experimental validation identify biomarkers related OA the aim targets. METHODS The series matrix file GSE82107 was downloaded from Gene Expression Omnibus (GEO) public databases. data were processed differentially expressed genes (DEGs) identified via R 4.2.2 software. related-DEGs subsequently defined as DEGs that overlapped autophagy-related selected on basis Venn diagram. screened Ontology (GO) analysis, Kyoto Encyclopedia Genes Genomes (KEGG) enrichment protein-protein interaction (PPI) immune cell infiltration analysis. Based constructed PPI network, hub CytoHubba plugin. Moreover, confirmed receiver operating characteristic (ROC) curve GSE129147 GSE169077 datasets. In addition, we collected synovial tissue knees ten patients: five patients who underwent total knee arthroplasty young arthroscopic surgery for anterior cruciate ligament rupture. samples subjected RNA extraction followed by real-time quantitative RT-PCR.Finally, verified expression HUB gene calculating corresponding mRNA levels. RESULTS Five namely, BCL2L1, CTSD, ATG3, GABARAPL2 CTSB related-DEGs. ROC revealed almost all had good diagnostic value, but Q-PCR results clinical GABARAPL2, upregulated (p < 0.05). relative expressions CTSD ATG3 not significantly different between group control > CONCLUSIONS This study OA, including CTSB. These are promising targets treatment.

Language: Английский

Metabolic reprogramming of fibroblast-like synoviocytes with a supramolecular nano-drug for osteoarthritis therapy DOI Creative Commons
Youguang Ma, Jiaxing Dong,

Xiangqian Zou

et al.

Advanced Composites and Hybrid Materials, Journal Year: 2025, Volume and Issue: 8(1)

Published: Feb. 1, 2025

Language: Английский

Citations

0
Bioactive nanocapsules based on lychee-like bilayer structure for synovitis treatment DOI Creative Commons

Wenwen Chai,

Pengfei Tian, Mengke Fan

et al.

Composites Part B Engineering, Journal Year: 2025, Volume and Issue: unknown, P. 112303 - 112303

Published: Feb. 1, 2025

Language: Английский

Citations

0
Intelligent Nanomaterials Design for Osteoarthritis Managements DOI Open Access
Zhihao Chen, Xuan Zheng, Zhengzhi Mu

et al.

Small Methods, Journal Year: 2025, Volume and Issue: unknown

Published: March 30, 2025

Osteoarthritis (OA) is the most prevalent degenerative joint disorder, characterized by progressive degradation, pain, and diminished mobility, all of which collectively impair patients' quality life escalate healthcare expenditures. Current treatment options are often inadequate due to limited efficacy, adverse side effects, temporary symptom relief, underscoring urgent need for more effective therapeutic strategies. Recent advancements in nanomaterials nanomedicines offer promising solutions improving drug bioavailability, reducing effects providing targeted benefits. This review critically examines pathogenesis OA, highlights limitations existing treatments, explores latest innovations intelligent design OA therapy, with an emphasis on their engineered properties, mechanisms, translational potential clinical application. By compiling recent findings, this work aims inspire further exploration innovation nanomedicine, ultimately advancing development personalized therapies.

Language: Английский

Citations

0
Lubricated injectable electroactive short fibers facilitate cartilage repair through piezoelectric conversion DOI

Xiaoqian Ding,

Jieliang Shen, Jindong Tan

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 162382 - 162382

Published: April 1, 2025

Language: Английский

Citations

0
Injectable Nanocomposite Hydrogel System for miRNA‐Based Cartilage Repair in Knee Osteoarthritis DOI
Dianbo Long, Guping Mao, Hong‐Yi Li

et al.

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 22, 2025

Abstract Knee osteoarthritis (KOA) is a common joint disorder characterized by progressive cartilage degeneration. MiR‐455‐3p has been identified as promising therapeutic candidate to maintain homeostasis. However, clinical application of free miR‐455‐3p limited rapid degradation, poor targeting efficiency, and off‐target effects. To overcome these limitations, an injectable nanocomposite hydrogel‐based miRNA delivery system consisting affinity nanocarrier (CANC) encapsulated within PCL‐b‐PEG‐b‐PCL hydrogel developed. The CANC formulated using 50% PEGylated G5 PAMAM dendrimers load further modified with chondrocyte‐affinity peptides (CAP) minimal “self” (MSP) enhance evade macrophage uptake. In vitro studies demonstrated that it exhibited excellent stability, low cytotoxicity, superior penetration chondrocyte targeting. Moreover, in vivo experiments knockout mice destabilization the medial meniscus (DMM) induced KOA model confirmed sustained release targeted effectively rescued degeneration prevent progression. Notably, high biocompatibility without observed cytotoxicity major organs. This study highlights potential promoting regeneration presents approach for development disease‐modifying drugs.

Language: Английский

Citations

0
Emerging Therapy in Osteoarthritis: Mesenchymal Stem Cells, Secretomes, and Using Hydrogels to Enhance Efficacy DOI

Qi-Ming Pang,

Jingdi Zhan,

Zhuolin Chen

et al.

BioDrugs, Journal Year: 2025, Volume and Issue: unknown

Published: May 30, 2025

Language: Английский

Citations

0
Identification of biomarkers related to autophagy in osteoarthritis via bioinformatics analysis and experimental validation (Preprint) DOI

Enhong Song,

Gang‐Ming Zou, Chong Shen

et al.

Published: Nov. 7, 2024

BACKGROUND Osteoarthritis(OA) is a degenerative disease with few effective early interventions and therapeutic options, autophagy thought to play significant role in progression of OA. OBJECTIVE We used bioinformatics analysis experimental validation identify biomarkers related OA the aim targets. METHODS The series matrix file GSE82107 was downloaded from Gene Expression Omnibus (GEO) public databases. data were processed differentially expressed genes (DEGs) identified via R 4.2.2 software. related-DEGs subsequently defined as DEGs that overlapped autophagy-related selected on basis Venn diagram. screened Ontology (GO) analysis, Kyoto Encyclopedia Genes Genomes (KEGG) enrichment protein-protein interaction (PPI) immune cell infiltration analysis. Based constructed PPI network, hub CytoHubba plugin. Moreover, confirmed receiver operating characteristic (ROC) curve GSE129147 GSE169077 datasets. In addition, we collected synovial tissue knees ten patients: five patients who underwent total knee arthroplasty young arthroscopic surgery for anterior cruciate ligament rupture. samples subjected RNA extraction followed by real-time quantitative RT-PCR.Finally, verified expression HUB gene calculating corresponding mRNA levels. RESULTS Five namely, BCL2L1, CTSD, ATG3, GABARAPL2 CTSB related-DEGs. ROC revealed almost all had good diagnostic value, but Q-PCR results clinical GABARAPL2, upregulated (p < 0.05). relative expressions CTSD ATG3 not significantly different between group control > CONCLUSIONS This study OA, including CTSB. These are promising targets treatment.

Language: Английский

Citations

0