Trienzyme‐in‐One Nanoparticle Making Multifunctional Synergistic Nanorobot for Tumor Therapy DOI
Zhixue Gao, Zili Yang, Ming Luo

et al.

Small Methods, Journal Year: 2025, Volume and Issue: unknown

Published: May 12, 2025

Abstract Current nanoparticle‐based drug delivery systems for tumor therapy face significant challenges in intratumoral penetration and cellular internalization, leading to poor therapeutic efficacy. Herein, it is demonstrated that the sequential integration of glucose oxidase (GOx), catalase (CAT), urease (URE) onto half surface biotin‐modified Janus nanoparticles via chemical coupling way produces nanorobots multifunctionality synergistic effect (denoted as UCGPJNRs). They can autonomously powerfully move microenvironment (TME) by using endogenous urea a fuel, enabling penetrate deeper than 0.55 mm into tissues, ≈5.5‐fold previous counterparts. The UCGPJNRs perform motion‐enhanced biotin receptor‐mediated endocytosis endoplasmic reticulum/Golgi apparatus pathway‐mediated exocytosis, greatly improving internalization efficiency cells. release NH 3 when moving produce selective toxicity against cells hypoxic TME. Further, they enhance consumption ≈three times due motion‐accelerated GOx/CAT cascade reaction, disrupting metabolism on large area. After intratumorally injecting tumor‐bearing mice, significantly amplify vivo growth inhibition rate through their effect. This work provides plausible strategy overcome current limitations treatment anchoring multiple bioenzymes one nanoparticle.

Language: Английский

Trienzyme‐in‐One Nanoparticle Making Multifunctional Synergistic Nanorobot for Tumor Therapy DOI
Zhixue Gao, Zili Yang, Ming Luo

et al.

Small Methods, Journal Year: 2025, Volume and Issue: unknown

Published: May 12, 2025

Abstract Current nanoparticle‐based drug delivery systems for tumor therapy face significant challenges in intratumoral penetration and cellular internalization, leading to poor therapeutic efficacy. Herein, it is demonstrated that the sequential integration of glucose oxidase (GOx), catalase (CAT), urease (URE) onto half surface biotin‐modified Janus nanoparticles via chemical coupling way produces nanorobots multifunctionality synergistic effect (denoted as UCGPJNRs). They can autonomously powerfully move microenvironment (TME) by using endogenous urea a fuel, enabling penetrate deeper than 0.55 mm into tissues, ≈5.5‐fold previous counterparts. The UCGPJNRs perform motion‐enhanced biotin receptor‐mediated endocytosis endoplasmic reticulum/Golgi apparatus pathway‐mediated exocytosis, greatly improving internalization efficiency cells. release NH 3 when moving produce selective toxicity against cells hypoxic TME. Further, they enhance consumption ≈three times due motion‐accelerated GOx/CAT cascade reaction, disrupting metabolism on large area. After intratumorally injecting tumor‐bearing mice, significantly amplify vivo growth inhibition rate through their effect. This work provides plausible strategy overcome current limitations treatment anchoring multiple bioenzymes one nanoparticle.

Language: Английский

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