Bioprinted vascular tissue: Assessing functions from cellular, tissue to organ levels

Materials Today Bio, Journal Year: 2023, Volume and Issue: 23, P. 100846 - 100846

Published: Oct. 28, 2023

3D bioprinting technology is widely used to fabricate various tissue structures. However, the absence of vessels hampers ability bioprinted tissues receive oxygen and nutrients as well remove wastes, leading a significant reduction in their survival rate. Despite advancements bioinks technologies, vascular structures continue be unsuitable for transplantation compared natural blood vessels. In addition, complete assessment index system evaluating structure function vitro has not yet been established. Therefore, this review, we firstly highlight significance selecting suitable techniques they two synergize with each other. Subsequently, focusing on both vascular-associated cells tissues, provide relatively thorough functions based physiological that possess. We end review applications models, such vessel-on-a-chip, simulating pathological processes conducting drug screening at organ level. believe development fully functional will soon make great contributions engineering regenerative medicine.

Language: Английский

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3D Bioprinting tissue analogs: Current development and translational implications

Journal of Tissue Engineering, Journal Year: 2023, Volume and Issue: 14

Published: Jan. 1, 2023

Three-dimensional (3D) bioprinting is a promising and rapidly evolving technology in the field of additive manufacturing. It enables fabrication living cellular constructs with complex architectures that are suitable for various biomedical applications, such as tissue engineering, disease modeling, drug screening, precision regenerative medicine. The ultimate goal to produce stable, anatomically-shaped, human-scale functional organs or substitutes can be implanted. Although techniques have emerged develop customized tissue-engineering over past decade, several challenges remain fabricating volumetric shapes sizes translating printed products into clinical practice. Thus, it crucial successful strategy research outputs practice address current organ crises improve patients’ quality life. This review article discusses existing processes preparing clinically relevant substitutes. further reviews strategies technical feasibility overcome limit biological their translational implications. Additionally, highlights exciting technological advances 3D anatomically shaped suggests future development directions. aims provide readers insight state-of-the-art powerful tools engineering tissues organs.

Language: Английский

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Gelation of Uniform Interfacial Diffusant in Embedded 3D Printing

Advanced Functional Materials, Journal Year: 2023, Volume and Issue: 33(50)

Published: Aug. 1, 2023

While the human body has many different examples of perfusable structures with complex geometries, biofabrication methods to replicate this complexity are still lacking. Specifically, fabrication self-supporting, branched networks multiple channel diameters is particularly challenging. Here, we present Gelation Uniform Interfacial Diffusant in Embedded 3D Printing (GUIDE-3DP) approach for constructing interconnected channels precise control over branching geometries and vessel sizes. To achieve user-specified dimensions, technique leverages predictable diffusion crosslinking reaction-initiators released from sacrificial inks printed within a hydrogel precursor. We demonstrate versatility GUIDE-3DP be adapted use diverse physicochemical mechanisms by designing seven printable material systems. Importantly, allows independent tunability both inner outer ability fabricate seamless junctions at branch points. This bioprinting platform uniquely suited fabricating lumenized shapes characteristic hollow vessels throughout body. As an exemplary application, vasculature-like lined endothelial cells. represents important advance toward physiologically relevant intricate geometries.

Language: Английский

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Integrating Computational and Biological Hemodynamic Approaches to Improve Modeling of Atherosclerotic Arteries

Advanced Science, Journal Year: 2024, Volume and Issue: 11(26)

Published: May 5, 2024

Abstract Atherosclerosis is the primary cause of cardiovascular disease, resulting in mortality, elevated healthcare costs, diminished productivity, and reduced quality life for individuals their communities. This exacerbated by limited understanding its underlying causes limitations current therapeutic interventions, highlighting need sophisticated models atherosclerosis. review critically evaluates computational biological atherosclerosis, focusing on study hemodynamics atherosclerotic coronary arteries. Computational account geometrical complexities blood vessels stenoses, but they fail to capture complex processes involved Different vitro vivo can aspects complexity healthy stenosed vessels, rarely mimic human anatomy physiological hemodynamics, require significantly more time, cost, resources. Therefore, emerging strategies are examined that integrate models, potential advances imaging, biofabrication, machine learning explored developing effective

Language: Английский

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Volumetric 3D Printing of Endoskeletal Soft Robots

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(33)

Published: June 14, 2024

Computed Axial Lithography (CAL) is an emerging technology for manufacturing complex parts, all at once, by circumventing the traditional layered approach using tomography. Overprinting, a unique additive capability of CAL, allows 3D geometry to be formed around prepositioned insert where occlusion light compensated other angular projections. This method opens door novel applications within multi-material systems such as endoskeletal robots. Herein, this work presents one application with simple Gelatin Methacrylate (GelMA)hydrogel osmotic actuator embedded system. GelMA ideal material it swellable and has reversible thermal gelation, enabling suspension endoskeleton during printing. By tuning formulation, design, post-processing, swelling-induced bending actuation 60 degrees achieved. To aid in printing process, computational determining absolute dose absorbed resin allowing print time prediction also proposed.

Language: Английский

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