Framework Nucleic Acids Combined with 3D Hybridization Chain Reaction Amplifiers for Monitoring Multiple Human Tear Cytokines

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(26)

Published: March 16, 2024

Abstract Existing tear sensors are difficult to perform multiplexed assays due the minute amounts of biomolecules in tears and tiny volume tears. Herein, authors leverage DNA tetrahedral frameworks (DTFs) modified on wireless portable electrodes effectively capture 3D hybridization chain reaction (HCR) amplifiers for automatic sensitive monitoring multiple cytokines human The developed allow determination various dry eye syndrome (DES)‐associated with limit detection down 0.1 pg mL −1 , consuming as little 3 fluid. Double‐blind testing clinical DES samples using sensor commercial ELISA shows no significant difference between them. Compared single‐biomarker diagnosis, diagnostic accuracy this based biomarkers has improved by ≈16%. system offers potential enable personalized accurate diagnosis ocular diseases.

Language: Английский

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Artificial Nanovesicles Derived from Cells: A Promising Alternative to Extracellular Vesicles

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 18, 2024

As naturally secreted vesicles by cells, extracellular (EVs) play essential roles in modulating cell–cell communication and have significant potential tissue regeneration, immune regulation, drug delivery. However, the low yield uncontrollable heterogeneity of EVs been obstacles to their widespread translation into clinical practice. Recently, it has discovered that artificial nanovesicles (NVs) produced cell processing can inherit components functions parent cells possess similar structures EVs, with significantly higher yields more flexible functionalization, making them a powerful complement natural EVs. This review focuses on recent advances research NVs as replacements for We provide an overview comparing summarize top-down preparation strategies NVs. The applications prepared from stem differentiated engineered are presented, well latest NV engineering. Finally, main challenges discussed.

Language: Английский

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The Role of Extracellular Vesicles in the Treatment of Prostate Cancer

Small, Journal Year: 2024, Volume and Issue: unknown

Published: April 19, 2024

Prostate cancer (PCa) has become a public health concern in elderly men due to an ever-increasing number of estimated cases. Unfortunately, the available treatments are unsatisfactory because lack durable response, especially advanced disease states. Extracellular vesicles (EVs) lipid-bilayer encircled nanoscale that carry numerous biomolecules (e.g., nucleic acids, proteins, and lipids), mediating transfer information. The past decade witnessed wide range EV applications both diagnostics therapeutics. First, EV-based non-invasive liquid biopsies provide biomarkers various clinical scenarios guide treatment; EVs can facilitate grading staging patients for appropriate treatment selection. Second, play pivotal role pathophysiological processes via intercellular communication. Targeting key molecules involved EV-mediated tumor progression proliferation, angiogenesis, metastasis, immune escape, drug resistance) is potential approach curbing PCa. Third, promising carriers. Naïve from sources engineered delivery systems have paved way development new modalities. This review discusses recent advancements application therapies highlights functional materials as novel interventions

Language: Английский

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SHINE: SERS-based Hepatotoxicity detection using Inference from Nanoscale Extracellular vesicle content

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 30, 2025

Extracellular vesicles (EV) are becoming crucial tools in liquid biopsy, diagnostics, and therapeutic applications, yet their nanoscale characterization remains challenging. In this context, the detection of drug-induced liver injury, i . e ., hepatotoxicity, through extracellular vesicle molecular content an unexplored frontier. To end, we present a label-free surface-enhanced Raman (SERS) spectroscopy approach, which provides rapid EV analysis under ten minutes requires only 1.3 microliters sample. Using hepatic cultures as model, our platform captures distinct reproducible changes response to acetaminophen-induced hepatoxicity. Our achieves exceptional accuracy with root mean squared error values low 3.80%, establishing strong correlations between spectra conventional toxicity biomarkers. Unlike previous EV-SERS studies limited identification disease markers, approach reveals drug-response signatures strongly correlated markers. These findings establish EVs dynamic reporters cellular drug responses demonstrate use SERS-based hepatotoxicity.

Language: Английский

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Recent advance on extracellular vesicle labeling: From strategy to probe

Coordination Chemistry Reviews, Journal Year: 2025, Volume and Issue: 534, P. 216601 - 216601

Published: March 10, 2025

Language: Английский

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Extracellular Vesicles as Precision Therapeutic Vectors: Charting the Future of Cell-Targeted Therapy

Precision medicine and engineering., Journal Year: 2025, Volume and Issue: unknown, P. 100031 - 100031

Published: May 1, 2025

Language: Английский

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Morphological Transformation and Surface Engineering of Glycovesicles Driven by Bioinspired Hydrogen Bonds of Nucleobases

ACS Macro Letters, Journal Year: 2024, Volume and Issue: 13(4), P. 468 - 474

Published: April 4, 2024

Glycopolymer-based supramolecular glycoassemblies with signal-driven cascade morphological deformation and accessible surface engineering toward bioinspired functional glycomaterials have attracted much attention due to their diverse applications in fundamental practical scenarios. Herein, we achieved the transformation of a nucleobase-containing polymeric glycovesicle through exploiting complementary multiple hydrogen bonds nucleobases. First, synthesized thymine-containing glycopolymers (PGal30-b-PTAm249) are capable self-assembling into well-defined glycovesicles. Several kinds amphiphilic adenine-containing block copolymers neutral, positive, negative charges were engineer glycovesicles between adenine thymine. A transformations from vesicles ruptured tails, worm-like micelles, finally spherical micelles observed via continuously adding polymer Furthermore, charge properties these glyconano-objects can be facilely regulated incorporating various polymers. This work demonstrates potential application unique approach precisely morphology for boosting biological applications.

Language: Английский

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Direct imaging with multidimensional labelling and high‐content analysis allows quantitative categorization and characterizations of individual small extracellular vesicles and nanoparticles (sEVPs)

Journal of Extracellular Vesicles, Journal Year: 2024, Volume and Issue: 13(12)

Published: Dec. 1, 2024

Small extracellular vesicles and nanoparticles (sEVPs) are cell-secreted entities with potential as diagnostic biomarkers therapeutic vehicles. However, significant intrinsic sEVP heterogeneity impedes analysis understanding of their composition functions. We employ multidimensional fluorescent labelling on sEVPs, leveraging the robustness a newly developed membrane probe-conjugated oligoelectrolytes (COEs), conduct total internal reflection fluorescence (TIRF) microscopy arrays. These arrays comprise single sEVPs anchored to soft material functionalized surface little bias. then develop an enhanced algorithm for colocalization multiple labels individual perform deep profiling particle content. categorize derived from same cell type into seven distinct subpopulations-some vesicular whereas others non-vesicular, we demonstrate that four types exhibit quantitatively distinguishable subpopulation distributions. Furthermore, gain insights specific features within each subpopulation, including CD63 counts, relative size, concentration cargoes, correlations among different cargoes. This high-content reveals common cargo sorting in subpopulations across suggests new statistics inherent could differentiate two cancer cells normal cells. Collectively, our study presents robust single-sEVP characterization platform, combining imaging comprehensive analysis. platform is poised advance sEVP-based theranostic assays facilitate exploration disease-associated biogenesis sEVP-mediated intercellular communication.

Language: Английский

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DNA‐Assisted Separation of Nanoparticles

Small Methods, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 24, 2024

DNA, as natural polymers, not only serves the genetic material but also shows great potential assistants for efficient and targeted nanoparticle separation. This is due to its high programmability, excellent biocompatibility, stability effectively binding with various types of nanoparticles or bioparticles, while modifying physical properties target particles such charge, mass size. Researchers have achieved remarkable efficiency in separating by precisely designing DNA sequences their interactions particles. achievement primarily relies on differences medium modulated DNA. In this minireview, diverse strategies are overviewed using assist separation, applications these separation highlighted. Finally, current challenges prospects DNA-assisted discussed.

Language: Английский

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Photoreceptor-targeted extracellular vesicles-mediated delivery of Cul7 siRNA for retinal degeneration therapy

Theranostics, Journal Year: 2024, Volume and Issue: 14(13), P. 4916 - 4932

Published: Jan. 1, 2024

Photoreceptor loss is a primary pathological feature of retinal degeneration (RD) with limited treatment strategies. RNA interference (RNAi) has emerged as promising method gene therapy in regenerative medicine. However, the transfer RNAi therapeutics to photoreceptors and deficiency effective therapeutic targets are still major challenges RD.

Language: Английский

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