Optical sorting: past, present and future

Light Science & Applications, Journal Year: 2025, Volume and Issue: 14(1)

Published: Feb. 27, 2025

Optical sorting combines optical tweezers with diverse techniques, including spectrum, artificial intelligence (AI) and immunoassay, to endow unprecedented capabilities in particle sorting. In comparison other methods such as microfluidics, acoustics electrophoresis, offers appreciable advantages nanoscale precision, high resolution, non-invasiveness, is becoming increasingly indispensable fields of biophysics, chemistry, materials science. This review aims offer a comprehensive overview the history, development, perspectives various categorised passive active methods. To begin, we elucidate fundamental physics attributes both conventional exotic forces. We then explore sorting, which fuses diversity Raman spectroscopy machine learning. Afterwards, reveal essential roles played by deterministic light fields, configured lens systems or metasurfaces, particles based on their varying sizes shapes, resolutions speeds. conclude our vision most promising futuristic directions, AI-facilitated ultrafast bio-morphology-selective It can be envisioned that will inevitably become revolutionary tool scientific research practical biomedical applications.

Language: Английский

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Optically Actuated Soft Microrobot Family for Single‐Cell Manipulation

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(32)

Published: May 30, 2024

Precisely controlled manipulation of nonadherent single cells is often a pre-requisite for their detailed investigation. Optical trapping provides versatile means positioning with submicrometer precision or measuring forces femto-Newton resolution. A variant the technique, called indirect optical trapping, enables single-cell no photodamage and superior spatial control stability by relying on optically trapped microtools biochemically bound to cell. High-resolution 3D lithography prepare such cell manipulators any predefined shape, greatly extending number achievable tasks. Here, it presented first time novel family that are deformable tweezers rely elasticity hold cells. This more straightforward approach avoiding biochemical functionalization attachment, consequently enabling manipulated be released at time. Using photoresist Ormocomp, deformations in tens pN range present three modes as examples showcase possible applications soft microrobotic tools can offer characterized. The describe here include collection, imaging, spatially temporally cell-cell interaction.

Language: Английский

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Advancing MicroRNA Detection: Enhanced Biotin–Streptavidin Dual-Mode Phase Imaging Surface Plasmon Resonance Aptasensor

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(21), P. 8791 - 8799

Published: May 14, 2024

MicroRNAs (miRNAs) are novel tumor biomarkers owing to their important physiological functions in cell communication and the progression of multiple diseases. Due small molecular weight, short sequence length, low concentration levels miRNA, miRNA detection presents substantial challenges, requiring advancement more refined sensitive techniques. There is an urgent demand for development a rapid, user-friendly, analysis method. Here, we developed enhanced biotin–streptavidin dual-mode phase imaging surface plasmon resonance (PI-SPR) aptasensor rapid miRNA. Initially, evaluated linear sensing range across two distinct modalities investigated physical factors that influence signal aptamer-miRNA interaction within PI-SPR aptasensor. Then, amplification strategy was introduced aptasensor, which effectively reduced nonspecific adsorption by 20% improved limit 548 times. Furthermore, have produced three types marker chips, utilize mode (less than 2 min) achieve simultaneous markers serum from clinical cancer patients. This work not only new approach detect different application scenarios but also provided reference system other biomolecules.

Language: Английский

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Thermophoresis‐Induced Polymer‐Driven Destabilization of Gold Nanoparticles for Optically Directed Assembly at Interfaces

Small Methods, Journal Year: 2024, Volume and Issue: unknown

Published: July 3, 2024

Abstract The limitations of conventional template‐based methods for the deposition nanoparticle assemblies into defined patterns on solid substrates call development techniques that do not require templates or lithographic masks. use optically‐induced thermal gradients to drive migration colloids toward away from a laser spot, known as opto‐thermophoresis, has shown promise low‐power trapping and optical manipulation variety colloidal species. However, printing using this technique so far been established. Herein, method optically directed noble metal nanoparticles, specifically gold nanospheres is reported. thermophoresis polymer polyvinylpyrrolidone spot led aggregates, capable serving surface‐enhanced Raman scattering substrates. influence heating power concentrations polymer, salt, surfactant rate structure printed pattern are studied, showing conditions can permit printing, suggesting facile generalization different compositions, sizes, shapes. These findings will greatly benefit future efforts assembly, applications in sensing, photothermal heating, relevant biomedicine devices.

Language: Английский

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Plasmonic Dipole and Quadrupole Scattering Modes Determine Optical Trapping, Optical Binding, and Swarming of Gold Nanoparticles

The Journal of Physical Chemistry C, Journal Year: 2024, Volume and Issue: 128(13), P. 5731 - 5740

Published: March 26, 2024

Laser trapping at an interface provides a unique platform for assembling novel multiparticle-based optical matter that extends well beyond the irradiated area. Optical binding, resulting from resonantly scattered photons by gold nanoparticles through dipolar scattering mode, serves as primary force supporting cohesion of particles in these optically induced assemblies, which is interpreted view formation binding network. Unfortunately, dipole mode restricted to narrow range experimental conditions, limiting its use specific laser wavelengths and particle sizes. To address this limitation, exploring higher multipole modes, such quadrupole, could provide broader conditions. In work, we will describe how quadrupole influences possibility extending potential outside area constructing large assemblies.

Language: Английский

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Artificial potential field-empowered dynamic holographic optical tweezers for particle-array assembly and transformation

PhotoniX, Journal Year: 2024, Volume and Issue: 5(1)

Published: Oct. 15, 2024

Abstract Owing to the ability parallel manipulate micro-objects, dynamic holographic optical tweezers (HOTs) are widely used for assembly and patterning of particles or cells. However, simultaneous control large-scale targets, potential collisions could lead defects in formed patterns. Herein we introduce artificial field (APF) develop HOTs that enable collision-avoidance micro-manipulation. By eliminating collision risks among particles, this method can maximize degree parallelism multi-particle transport, it permits implementation Hungarian algorithm matching with their target sites a minimal pathway. In proof-of-concept experiments, employ APF-empowered achieve direct defect-free 8 × array microbeads, which starts from random initial positions. We further demonstrate successive flexible transformations 7 microbead array, by regulating its tilt angle inter-particle spacing distances minimalist path. anticipate proposed will become versatile tool open up new possibilities micromanipulation tasks variety fields.

Language: Английский

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Power Micromachines With Light

Laser & Photonics Review, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 29, 2024

Abstract Optical manipulation technology encompasses a suite of micromanipulation techniques that employ light to control and actuate microscopic objects. As valuable scientific tool, optical is employed by researchers investigate fundamental biological processes, examine the mechanics microstructures, develop innovative technologies with applications in diagnostics, imaging, micro‐scale manufacturing. The rapid development technology, combined advanced microfabrication techniques, has catalyzed emergence burgeoning research domain termed optically‐driven micromachinery. This rapidly expanding field garnered significant interest recent years, fostering interdisciplinary collaboration across manufacturing, materials science, biotechnology, micro‐electromechanical systems. capability optically manipulate micromachines also opens new avenues for tools, sensors, devices enhanced functionalities, enabling accomplishment tasks previously considered impossible. review presents systematic overview two important technologies, tweezers optoelectronic tweezers, focus on their A comparative analysis conducted, accompanied discussion strategies further enhance performance, paving way more powerful micromachinery future.

Language: Английский

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Optimizing Light Pattern Curvature to improve the performance of Optoelectronic Tweezers in micromanipulation

Optics Express, Journal Year: 2025, Volume and Issue: 33(2), P. 2968 - 2968

Published: Jan. 10, 2025

Optoelectronic tweezers (OET) offer a versatile, programmable, and contactless method for manipulating microscale objects. While factors like AC voltage light intensity have been extensively studied, the role of pattern curvature in performance OET manipulation remains underexplored. This study investigates how patterns affects movement polystyrene microparticles under negative dielectrophoretic (DEP) forces an system. Experimental results show that as decreases, maximum velocity first increases to peak then gradually decreases. Numerical simulations reveal significantly influences horizontal vertical DEP forces, altering equilibrium positions velocities. By defining optimal (χ, ratio microparticle diameter inner pattern), we found achieve stability at this regardless sizes. These findings key insights into optimizing improved performance, facilitating more precise efficient applications micromanipulation, micro-assembly, microfabrication, beyond.

Language: Английский

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Crossing the Dimensional Divide with Optoelectronic Tweezers: Multicomponent Light‐Driven Micromachines with Motion Transfer in Three Dimensions

Advanced Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 13, 2025

Abstract Micromachines capable of performing diverse mechanical tasks in complex and constrained microenvironments are great interest. Despite important milestones this pursuit, until now, micromachines confined to actuation within a single 2D plane due the challenges transferring motion across different planes limited space. Here, breakthrough method is presented overcome limitation: multi‐component that facilitate 3D transfer planes. These light‐driven micromachines, fabricated using standard photolithography combined with direct laser writing, assembled actuated via programmable light patterns an optoelectronic tweezers system. Utilizing charge‐induced repulsion dielectrophoretic levitation effects, enable highly efficient rotation effective inter‐component transfer. Through work, fascinating similarities unveiled for new microscale systems when compared macro‐scale world which they live, paving way development micromechanical devices microsystems ever increasing functionality versatility.

Language: Английский

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Co‐Solvent‐Aided Opto‐Thermophoretic Printing of Gold Nanorod Assemblies

Advanced Optical Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 19, 2025

Abstract The controlled deposition of plasmonic nanoparticles on solid surfaces is critical for the fabrication medical diagnostics, devices, and sensors. Despite recent advances in harnessing thermal gradients to achieve at a laser spot, anisotropic gold by this approach challenge aqueous solution. Herein, use ethanol as co‐solvent shown enable rapid (<10 s) effective nanorods presence polyvinylpyrrolidone unmodified glass substrates. influence key parameters, including polymer concentration molecular weight, content, nanoparticle concentration, power, studied. While content size have largest impact assembly structure, all an growth rate. Polymer found significantly degree nanorod pattern, which leads surface‐enhanced Raman scattering enhancement factor up 10 8 . In summary, utilizes direct formation highly localized assemblies without need functionalization substrate. This simple, fast, adaptable promising platform scalable patterning nanostructures.

Language: Английский

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