Tumor perfusion enhancement by microbubbles ultrasonic cavitation reduces tumor glycolysis metabolism and alleviate tumor acidosis DOI Creative Commons

Danxia Qiu,

Yangcheng He,

Yuyi Feng

et al.

Frontiers in Oncology, Journal Year: 2024, Volume and Issue: 14

Published: July 18, 2024

The tumor microenvironment is increasingly acknowledged as a critical contributor to cancer progression, mediating genetic and epigenetic alterations. Beyond diverse cellular interactions from the microenvironment, physicochemical factors such acidosis also significantly affect dynamics. Recent research has highlighted that facilitates invasion, immune escape, metastasis, resistance therapies. Thus, noninvasive measurement of acidity development targeted interventions represent promising strategies in oncology. Techniques like contrast-enhanced ultrasound (CEUS) can effectively assess blood perfusion, while ultrasound-stimulated microbubble cavitation (USMC) proven enhance perfusion. We therefore aimed determine whether CEUS assesses USMC treatment modulate acidity. Firstly, we tracked perfusion parameters MCF7 models compared them with vivo pH recorded by microsensors. found peak intensity area under curve correlated well pH. further conducted on tumor-bearing mice, changes different regions before after its impact optimize therapeutic pressure. discovered 1.0 Mpa improved Furthermore, vascular pathology PGI2 assays indicated was mainly due vasodilation rather than angiogenesis. More importantly, analysis glycolysis-related metabolites enzymes demonstrated reduce reducing glycolysis. These findings support may serve potential biomarker modality for acidosis.

Language: Английский

Targeted delivery of MerTK protein via cell membrane engineered nanoparticle enhances efferocytosis and attenuates atherosclerosis in diabetic ApoE−/− Mice DOI Creative Commons
Shuo Qiu,

Jiahan Liu,

Jianmei Chen

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: April 13, 2024

Abstract Background Clearance of apoptotic cells by efferocytosis is crucial for prevention atherosclerosis progress, and impaired contributes to the aggravated atherosclerosis. Results In this study, we found that diabetic ApoE –/– mice showed as hyperglycemia damaged capacity at least partially due decreased expression Mer tyrosine kinase (MerTK) on macrophages. To locally restore MerTK in macrophages plaque, hybrid membrane nanovesicles (HMNVs) were thus developed. Briefly, cell from overexpressing RAW264.7 transferrin receptor (TfR) HEK293T mixed with DOPE polymers produce designated HMNVs. HMNVs could fuse recipient increased macrophages, which turn restored capacity. Upon intravenous administration into mice, superparamagnetic iron oxide nanoparticles (SMN) decorated accumulated aorta site significantly under magnetic navigation, where cleared efficiently inflammation. Conclusions Our study indicates decrease regional restoration plaque via be a promising therapeutic approach. Graphic

Language: Английский

Citations

7
Open‐Source Throttling of CD8+ T Cells in Brain with Low‐Intensity Focused Ultrasound‐Guided Sequential Delivery of CXCL10, IL‐2, and aPD‐L1 for Glioblastoma Immunotherapy DOI
Lei Dong, Yini Zhu,

Haoge Zhang

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(44)

Published: Sept. 12, 2024

Abstract Improving clinical immunotherapy for glioblastoma (GBM) relies on addressing the immunosuppressive tumor microenvironment (TME). Enhancing CD8 + T cell infiltration and preventing its exhaustion holds promise effective GBM immunotherapy. Here, a low‐intensity focused ultrasound (LIFU)‐guided sequential delivery strategy is developed to enhance cells activity in region. The of CXC chemokine ligand 10 (CXCL10) recruit interleukin‐2 (IL‐2) reduce their termed an “open‐source throttling” strategy. Consequently, up 3.39‐fold are observed with LIFU‐guided CXCL10, IL‐2, anti‐programmed death 1 (aPD‐L1), compared free aPD‐L1 group. immune checkpoint inhibitors (ICIs) therapeutic efficacy substantially enhanced by reversed TME due expansion cells, resulting elimination tumor, prolonged survival time, long‐term memory establishment orthotopic mice. Overall, cytokine ICIs offers which may present promising brain‐tumor

Language: Английский

Citations

6
Current status and future prospects of molecular imaging in targeting the tumor immune microenvironment DOI Creative Commons
Xiang Wang, Wen Shen, Lan Yao

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 22, 2025

Molecular imaging technologies have significantly transformed cancer research and clinical practice, offering valuable tools for visualizing understanding the complex tumor immune microenvironment. These allow non-invasive examination of key components within microenvironment, including cells, cytokines, stromal providing crucial insights into biology treatment responses. This paper reviews latest advancements in molecular imaging, with a focus on its applications assessing interactions Additionally, challenges faced by are discussed, such as need highly sensitive specific agents, issues data integration, difficulties translation. The future outlook emphasizes potential to enhance personalized through integration artificial intelligence development novel probes. Addressing these is essential fully realizing improving diagnosis, treatment, patient outcomes.

Language: Английский

Citations

0
Shenqi Funeng Xingnao Prescription Alleviates Exercise-Induced Fatigue by Regulating the HIF-1/FoxO Signaling Pathway DOI

Dong Xu,

Kai Gao, Wei Zhang

et al.

Journal of Ethnopharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 119881 - 119881

Published: April 1, 2025

Language: Английский

Citations

0
n‐Butanol Enables OMVs as Heat‐Responsive Nanometer Ultrasound Contrast Agents for Tumor Imaging DOI
Lujiao Wang,

Song Yang,

Tao Zhou

et al.

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: May 24, 2025

Abstract Extracellular vesicles, such as Escherichia coli outer membrane vesicles (OMVs), have been widely explored carriers for contrast agents, yet their application in ultrasound imaging remains limited. In this study, heat‐responsive nB‐OMVs are developed by loading n‐butanol into OMVs (nB‐OMVs). Upon heating, the decreased solubility of triggered phase separation within resulting enhanced signals, including fundamental, harmonic, and subharmonic components. Experimental results demonstrated that retained native morphology OMVs, along with tumor‐targeting accumulation tumor growth inhibition capabilities. injection induces red blood cell extravasation tumors, under near‐infrared (NIR) irradiation, these extravasated RBCs acted photothermal mediators, generating a localized thermal environment activated intratumoral further amplified signal. B‐mode contrast‐enhanced (CEUS) imaging, grayscale intensities increased 4.6‐ 8.5‐fold, respectively. This work provides promising strategy ultrasound‐based using extracellular vesicles.

Language: Английский

Citations

0
CXCR4-Targeted Ultrasound Microbubbles for Imaging and Enhanced Chemotherapy/Immunotherapy in Liver Cancer DOI
Yijie Qiu,

Jiaying Cao,

Jyh‐Fei Liao

et al.

Acta Biomaterialia, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

0
Tumor perfusion enhancement by microbubbles ultrasonic cavitation reduces tumor glycolysis metabolism and alleviate tumor acidosis DOI Creative Commons

Danxia Qiu,

Yangcheng He,

Yuyi Feng

et al.

Frontiers in Oncology, Journal Year: 2024, Volume and Issue: 14

Published: July 18, 2024

The tumor microenvironment is increasingly acknowledged as a critical contributor to cancer progression, mediating genetic and epigenetic alterations. Beyond diverse cellular interactions from the microenvironment, physicochemical factors such acidosis also significantly affect dynamics. Recent research has highlighted that facilitates invasion, immune escape, metastasis, resistance therapies. Thus, noninvasive measurement of acidity development targeted interventions represent promising strategies in oncology. Techniques like contrast-enhanced ultrasound (CEUS) can effectively assess blood perfusion, while ultrasound-stimulated microbubble cavitation (USMC) proven enhance perfusion. We therefore aimed determine whether CEUS assesses USMC treatment modulate acidity. Firstly, we tracked perfusion parameters MCF7 models compared them with vivo pH recorded by microsensors. found peak intensity area under curve correlated well pH. further conducted on tumor-bearing mice, changes different regions before after its impact optimize therapeutic pressure. discovered 1.0 Mpa improved Furthermore, vascular pathology PGI2 assays indicated was mainly due vasodilation rather than angiogenesis. More importantly, analysis glycolysis-related metabolites enzymes demonstrated reduce reducing glycolysis. These findings support may serve potential biomarker modality for acidosis.

Language: Английский

Citations

1