Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 370, P. 614 - 625
Published: May 14, 2024
Language: Английский
Chemistry of Materials, Journal Year: 2025, Volume and Issue: unknown
Published: April 11, 2025
Language: Английский
Citations
0
Biomaterials Science, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
This review highlights emerging strategies for lung-targeted mRNA delivery using non-viral vehicles, describing advances in bioinspired design and nanoparticle engineering.
Language: Английский
Citations
0
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown
Published: April 19, 2025
Abstract Endothelial cells (EC) comprise the pulmonary vascular bed and play a significant role in health disease. Consequently, EC niche represents an attractive therapeutic target for treating wide range of diseases. We have identified new class dicationic Charge-Altering Releasable Transporters. These single-component transporters selectively deliver mRNA to lung upon intravenous administration without use targeting ligand. Significantly, number spatial array cationic charges within repeating units CART polymer are found control both delivery efficacy tissue tropism. High-resolution imaging revealed efficient endothelial arteries, veins capillaries. The selective tropism these CARTs, coupled with tunable synthesis this family amphiphiles, enabling platform research clinical applications.
Language: Английский
Citations
0
ACS Applied Nano Materials, Journal Year: 2025, Volume and Issue: unknown
Published: May 1, 2025
Language: Английский
Citations
0
Nano Letters, Journal Year: 2024, Volume and Issue: unknown
Published: April 10, 2024
Two camps have emerged for targeting nanoparticles to specific organs and cell types: affinity moiety physicochemical tropism. Here we directly compare combine both using intravenous (IV) lipid (LNPs) designed target the lungs. We utilized PECAM antibodies as moieties cationic lipids These methods yield nearly identical lung uptake, but aPECAM LNPs show higher endothelial specificity. combining these had >2-fold uptake than either method alone markedly enhanced epithelial uptake. To determine if is because lungs are first organ downstream of IV injection, compared vs intra-arterial (IA) injection into carotid artery, finding that IA combined-targeting achieve 35% injected dose per gram (%ID/g) in first-pass organ, brain, among highest reported. Thus, strategies provides benefits neither achieves alone.
Language: Английский
Citations
3
Nature Biotechnology, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 5, 2025
Language: Английский
Citations
0
Nano Letters, Journal Year: 2025, Volume and Issue: unknown
Published: March 14, 2025
It is well established that the physicochemical properties of lipid nanoparticles (LNPs) can govern their interactions with various biological barriers. One property hypothesized to influence nanoparticle-cell elasticity. Here, we formulate LNPs naturally occurring cholesterol analogs tune LNP elasticity and study its role on mRNA delivery placenta. was measured via atomic force microscopy where these exhibited Young's moduli ranging from 71.0 ± 26.2 411.4 145.7 kPa. In vitro screening in placental trophoblasts showed stiffer improved uptake compared softer LNPs. Following intravenous administration pregnant mice, incorporating β-sitosterol enhanced reduced liver containing only cholesterol. These results demonstrate ability promote highlight potential tuning improve LNP-mediated organs interest.
Language: Английский
Citations
0
ACS Nano, Journal Year: 2025, Volume and Issue: unknown
Published: April 4, 2025
In the past 10 years, CRISPR-Cas9 has revolutionized gene-editing field due to its modularity, simplicity, and efficacy. It been applied for creation of in vivo models, further understand human biology, toward curing genetic diseases. However, there remain significant delivery barriers application clinic, especially extrahepatic applications. this work, high-throughput molecular barcoding techniques were used alongside traditional screening methodologies simultaneously evaluate LNP formulations encapsulating ribonucleoproteins (RNPs) vitro efficiency biodistribution. This resulted identification a lung-tropic formulation, which shows efficient gene editing endothelial epithelial cells within lung, targeting both model reporter clinically relevant genomic targets. Further, no off-target indel formation liver, making it highly specific system lung-editing
Language: Английский
Citations
0
Journal of Controlled Release, Journal Year: 2025, Volume and Issue: unknown, P. 113687 - 113687
Published: April 1, 2025
Language: Английский
Citations
0
Nanomedicine, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 27
Published: April 7, 2025
mRNA-based therapies have emerged as a transformative tool in modern medicine, gaining significant attention following their successful use COVID-19 vaccines. Delivery to the lungs offers several compelling advantages for mRNA delivery. The are one of most vascularized organs body, which provides an extensive surface area that can facilitate efficient drug transport. Local delivery bypasses gastrointestinal degradation, potentially enhancing therapeutic efficacy. In addition, capillary network ideal target systemic However, developing effective presents challenges. complex anatomy and body's immune response foreign particles create barriers This review discusses key approaches overcoming these challenges improving lungs. It examines both local strategies aimed at lung while mitigating off-target effects. Although substantial progress has been made lung-targeted therapies, remain optimizing cellular uptake achieving efficacy within pulmonary tissues. continued refinement enhance lung-specific targeting minimizing degradation is critical clinical success therapies.
Language: Английский
Citations
0