Langmuir,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 2, 2025
Amyloid
fibrils
have
recently
emerged
as
promising
building
blocks
for
functional
materials
due
to
their
exceptional
physicochemical
stability
and
adaptable
properties.
These
protein-based
structures
can
be
functionalized
create
hybrid
with
a
diverse
range
of
applications.
Here
we
report
simple
eco-friendly
protocol
generating
amyloid
from
hen
egg
white
lysozyme
decorated
gold
nanoparticles
that
self-assemble
in
hydrogel.
Reactive
oligomeric
species
act
reducing
agents,
enabling
the
efficient
formation
small
without
need
harsh
reagents.
Furthermore,
protein
molecules
template
nanoparticles,
which
are
stabilized
at
regular
intervals
along
fibril
axis,
preserving
nanoparticle
properties
macroscopic
scale.
As
an
illustration
potential
application,
show
hydrogel
employed
sense
quantify
creatinine
using
fluorescence
detection.
findings
reinforce
growing
interest
utilizing
proteins
foundational
elements
biomaterials
high
biocompatibility,
availability,
ability
finely
tune
supramolecular
assemblies.
Biomacromolecules,
Journal Year:
2025,
Volume and Issue:
26(1), P. 514 - 527
Published: Jan. 2, 2025
Piezoelectric
organic
polymers
are
promising
alternatives
to
their
inorganic
counterparts
due
mechanical
flexibility,
making
them
suitable
for
flexible
and
wearable
piezoelectric
devices.
Biological
such
as
proteins
have
been
reported
possess
piezoelectricity,
while
offering
additional
benefits,
biocompatibility
biodegradability.
However,
questions
remain
regarding
protein
the
impact
of
secondary
structure.
This
study
examines
properties
lysozyme
amyloid
fibril
films,
plasticized
by
polyethylene
glycol
(PEG).
The
films
demonstrated
a
measurable
d33
coefficient
1.4
±
0.1
pCN–1,
optimized
PEG
concentration,
confirming
piezoelectricity.
was
found
hydrogen-bond
with
fibrils,
likely
impacting
response
film.
Polarization
imaging
revealed
long-range
alignment
fibrils
in
circumferential
arrangement.
These
results
demonstrate
potential
using
which
can
be
formed
from
various
proteins,
create
bulk
self-assembled
materials.
ACS Synthetic Biology,
Journal Year:
2024,
Volume and Issue:
13(8), P. 2295 - 2312
Published: July 13, 2024
Engineered
Living
Materials
(ELMs)
are
materials
composed
of
or
incorporating
living
cells
as
essential
functional
units.
These
can
be
created
using
bottom-up
approaches,
where
engineered
spontaneously
form
well-defined
aggregates.
Alternatively,
top-down
methods
employ
advanced
science
techniques
to
integrate
with
various
kinds
materials,
creating
hybrids
and
intricately
combined.
ELMs
blend
synthetic
biology
science,
allowing
for
dynamic
responses
environmental
stimuli
such
stress,
pH,
humidity,
temperature,
light.
exhibit
unique
"living"
properties,
including
self-healing,
self-replication,
adaptability,
making
them
highly
suitable
a
wide
range
applications
in
medicine,
conservation,
manufacturing.
Their
inherent
biocompatibility
ability
undergo
genetic
modifications
allow
customized
functionalities
prolonged
sustainability.
This
review
highlights
the
transformative
impact
over
recent
decades,
particularly
healthcare
protection.
We
discuss
current
preparation
methods,
use
endogenous
exogenous
scaffolds,
assembly,
3D
bioprinting,
electrospinning.
Emphasis
is
placed
on
ongoing
research
technological
advancements
necessary
enhance
safety,
functionality,
practical
applicability
real-world
contexts.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 15, 2025
Abstract
Gut
bacteria
have
emerged
as
silent
drivers
in
the
pathology
of
Alzheimer’s
disease
(AD).
They
also
make
amyloids
with
structure
analogue
to
pathological
and
potential
cross-seed
propagate
a
prion-like
manner.
AD
is
characterised
by
accumulation
mature
extracellular
Amyloid-β
(Aβ)
plaques
which
are
surrounded
inflammatory
microglia.
We
report
that
exposure
interspecies
microbial
FapC
(fimbriae)
CsgA
(curli)
from
opportunistic
gut
pathogens
Pseudomonas
aeruginosa
Escherichia
coli
hyperactivates
microglia
against
Aβ
fibrils.
Microbial
fibrils
converge
phagocytic
compartments
through
subsequent
internalization,
not
observed
alone.
This
convergence
promotes
pro-inflammatory
defective
proteome
similar
those
brains.
The
resulting
clusters
develop
pro-inflammatory,
indigestible
interactome
eventually
regurgitated,
inducing
progressive
degeneration
bystander
neurons
ultimately
leading
cognitive
decline.
Collectively,
these
findings
provide
compelling
evidence
can
trigger
microglia-driven
neuroinflammation.
Molecular Aspects of Medicine,
Journal Year:
2025,
Volume and Issue:
103, P. 101351 - 101351
Published: March 1, 2025
The
amyloid
state
of
proteins
occurs
in
many
different
contexts
Nature
and
modern
society,
ranging
from
the
pathological
kind
(neurodegenerative
diseases
amyloidosis)
via
man-made
forms
(food
processing
-
to
a
much
smaller
extent
protein
biologics)
functional
versions
(bacterial
biofilm,
peptide
hormones
signal
transmission).
These
classes
all
come
together
human
body
which
endogenously
produces
amyloidogenic
able
form
(PaHA),
hosts
microbiome
continuously
makes
bacterial
(FuBA)
ingests
food
can
contain
amyloid.
This
have
grave
consequences,
given
that
PaHA
spread
throughout
"hand-me-down"
fashion
cell
through
small
fragments,
kick-start
growth
new
wherever
they
encounter
monomeric
precursors.
Amyloid
also
self-
cross-seed
across
dissimilar
sequences.
While
it
is
very
unlikely
ingested
plays
role
this
crosstalk,
FuBA-PaHA
interactions
are
increasingly
implicated
vivo
propagation.
We
now
position
understand
structural
bioinformatic
basis
for
cross-talk,
thanks
recently
obtained
atomic-level
structures
two
major
FuBAs
CsgA
(E.
coli)
FapC
(Pseudomonas).
there
reports
homology-driven
heterotypic
between
PaHA,
proteome
does
not
harbor
significant
homology
FapC.
Yet
we
others
uncovered
cross-stimulation
(and
some
cases
inhibition)
FuBA
both
vitro
vivo,
here
rationalize
based
on
structure
sequence.
important
consequences
transmission
development
neurodegenerative
diseases,
least
because
into
contact
gut-brain
interface,
recurrent
infections
with
microbes
potentially
even
invasive
biofilm
brain.
Whether
first
interact
gut
or
brain,
stimulate
block
each
other's
aggregation
as
well
trigger
inflammatory
responses.
may
affect
amyloidogenesis
other
ways,
e.g.
their
own
chaperones
recognize
show
experimentally
computationally.
Heterotypic
within
vital
part
phenomenon
constitute
vibrant
exciting
frontier
future
research.
Colloids and Surfaces B Biointerfaces,
Journal Year:
2025,
Volume and Issue:
251, P. 114617 - 114617
Published: March 7, 2025
Hydrogel
biomaterials
have
been
extensively
explored
for
applications
in
medicine,
materials
science,
and
the
development
of
functionalized
materials.
Traditionally,
hydrogels
were
produced
using
simple
polymers,
but
advancements
over
recent
decades
enabled
use
biological
such
as
proteins,
peptides,
polysaccharides,
even
amyloid
fibrils.
Among
these,
amyloid-based
demonstrated
unique
advantages,
including
enhanced
cell
adhesion
differentiation.
Furthermore,
they
can
be
engineered
living
bacteria
capable
producing
repairing
hydrogel
situ.
Here
we
investigate
novel
strategies
controlling
fibrillation
functional
CsgA.
We
designed
fusion
proteins
combining
two
CsgA
moieties
to
explore
methods
creating
branched
fibril
networks.
Our
approach
utilized
distinct
strategies:
passive
active
branching.
The
strategy
involved
direct
separated
by
a
alpha-helical
linker
integrate
into
fibrils
without
external
intervention.
branching
incorporated
redox-sensitive
variant
containing
an
internal
disulfide
bridge
that
blocks
until
reduced.
This
design
allows
precise
control
variants.
analyzed
these
constructs
qualitatively
combination
transmission
electron
microscopy
(TEM),
real-time
atomic
force
(AFM),
total
reflection
fluorescence
(TIRF)
microscopy,
supported
quantitative
image
analysis.
While
did
not
observe
evidence
branching,
our
modifications
led
significant
changes
behavior.
Notably,
TIRF
imaging
revealed
marked
increase
high-density
regions
following
activation
constructs,
indicating
potential
controlled
assembly
higher-order
structures.
These
findings
provide
new
insights
suggest
alternative
manipulating
organization.
observed
ability
alter
local
density
through
chemical
triggers
offers
promising
directions
developing
responsive
biomaterials.
propose
refinements
future
optimize
next-generation
biomaterial
applications.
Exploration,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 6, 2025
ABSTRACT
Inorganic
protein
hybrid
materials
(IPHMs)
due
to
editable
structure
present
unrivalled
potential
at
the
intersection
of
synthetic
biology
and
science.
The
synthesis
IPHMs
with
a
high
degree
biosafety
from
bioactive
units
represents
shift
in
material
design
synthesis.
This
paper
focuses
on
review
structural
basis
principles
proteins
for
specific
physical
chemical
functions.
It
also
provides
valuable
reference
emerging
through
conformational
relationship
IPHMs,
which
extends
applications
IPHMs.
In
addition,
construction
strategy
reaction
system
is
analyzed
perspective
biology.
possibility
engineering
batch
discussed.
Based
physicochemical
properties
different
applied‐oriented
research
biomedical
optical
imaging
multimodal
therapy,
idea
situ
proposed.
Ultimately,
trends
challenges
are
speculated
detail.
ACS Applied Polymer Materials,
Journal Year:
2025,
Volume and Issue:
7(6), P. 3739 - 3751
Published: March 12, 2025
Amyloid
peptides
are
structurally
diverse
materials
that
exhibit
different
properties
depending
on
their
self-assembly.
While
they
often
associated
with
neurodegenerative
diseases,
functional
amyloids
play
important
roles
in
nature
and
high
relevance
for
biomedical
applications,
including
remarkable
strength,
mechanical
stability,
antimicrobial
antioxidant
properties,
low
cytotoxicity,
adhesion
to
biotic
abiotic
surfaces.
Challenges
developing
amyloid
biomaterials
include
the
complexity
of
peptide
chemistry
practical
techniques
required
processing
into
bulk
materials.
In
this
work,
two
de
novo
decapeptides
fibrillar
globular
morphologies
were
synthesized,
blended
poly(ethylene
oxide),
fabricated
composite
mats
via
electrospinning.
Notable
enhancements
observed,
attributed
uniform
distribution
assemblies
within
PEO
matrix
interactions
between
Morphological
differences,
such
as
production
thinner
nanofibers,
increased
conductivity
from
zwitterionic
decapeptides.
Blend
rheology
postprocessing
analysis
revealed
how
might
affect
aggregation
secondary
structure
peptides.
Both
demonstrated
cytotoxicity
strong
activity,
indicating
potential
safe
effective
use
biomaterials.
This
research
lays
foundation
designing
specific
applications
by
defining
structure-property-processing
relationships
peptide-polymer
blends.
