Structural information in therapeutic peptides: Emerging applications in biomedicine

FEBS Open Bio, Journal Year: 2024, Volume and Issue: 15(2), P. 254 - 268

Published: June 14, 2024

Peptides are attracting a growing interest as therapeutic agents. This trend stems from their cost-effectiveness and reduced immunogenicity, compared to antibodies or recombinant proteins, but also ability dock interfere with large protein-protein interaction surfaces, higher specificity better biocompatibility relative organic molecules. Many tools have been developed understand, predict, engineer peptide function. However, most state-of-the-art approaches treat peptides only linear entities disregard structural arrangement. Yet, details critical for properties such solubility, stability, binding affinities. Recent advances in structure prediction successfully addressed the scarcity of confidently determined structures. review will explore different biotechnological applications assemblies, emphasizing importance integrating information advance these endeavors effectively.

Language: Английский

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Sustainable Smart Packaging from Protein Nanofibrils

Advanced Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 20, 2024

Abstract Smart packaging technologies are revolutionizing the food industry by extending shelf life and enhancing quality monitoring through environmental responsiveness. Here, a novel smart concept is presented, based on amyloid fibrils (AM) red radish anthocyanins (RRA), to effectively monitor spoilage color change. A protein nanofibrils biofilm developed from whey protein, which functionalized with RRA endow resulting films advanced capabilities. comprehensive characterization, including pH responsiveness, water vapor permeability, thermal mechanical testing, colorimetric responses, demonstrates superiority of AM/RRA compared control monomer building blocks. The findings indicate that can monitor, for example, shrimp freshness, showing visible changes within one day at room temperature significant alterations in after two days. Furthermore, these exhibit high antibacterial antioxidant activities, reinforcing their suitability efficient packaging. By integrating bio‐based materials natural anthocyanins, this research presents biodegradable, sustainable, cost‐effective solution, contributing eco‐friendly innovations preservation.

Language: Английский

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Interaction of Gut-Microbial Amyloids with Endogenous Amyloids can Drives Microglial Hyperactivity and Neuroinflammation in Alzheimer’s Disease

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

Abstract Gut bacteria have emerged as silent drivers in the pathology of Alzheimer’s disease (AD). They also make amyloids with structure analogue to pathological and potential cross-seed propagate a prion-like manner. AD is characterised by accumulation mature extracellular Amyloid-β (Aβ) plaques which are surrounded inflammatory microglia. We report that exposure interspecies microbial FapC (fimbriae) CsgA (curli) from opportunistic gut pathogens Pseudomonas aeruginosa Escherichia coli hyperactivates microglia against Aβ fibrils. Microbial fibrils converge phagocytic compartments through subsequent internalization, not observed alone. This convergence promotes pro-inflammatory defective proteome similar those brains. The resulting clusters develop pro-inflammatory, indigestible interactome eventually regurgitated, inducing progressive degeneration bystander neurons ultimately leading cognitive decline. Collectively, these findings provide compelling evidence can trigger microglia-driven neuroinflammation.

Language: Английский

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Mineralized aggregates based on native protein phase transition for non-destructive diagnosis of seborrheic skin by surface-enhanced Raman spectroscopy

Materials Horizons, Journal Year: 2024, Volume and Issue: 11(20), P. 5017 - 5030

Published: Jan. 1, 2024

A biomimetic mineralized aggregates strategy has been proposed, which could obtain molecular information about sebum by surface-enhanced Raman spectroscopy.

Language: Английский

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Application of metallic nanoparticles-amyloid protein supramolecular materials in tissue engineering and drug delivery: Recent progress and perspectives

Colloids and Surfaces B Biointerfaces, Journal Year: 2024, Volume and Issue: 244, P. 114185 - 114185

Published: Aug. 28, 2024

Language: Английский

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Mining and engineering activity in catalytic amyloids

Methods in enzymology on CD-ROM/Methods in enzymology, Journal Year: 2024, Volume and Issue: unknown, P. 345 - 422

Published: Jan. 1, 2024

Language: Английский

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Amyloid, a Jekyll and Hyde Molecule Inducing Neuronal Decline and Cognitive Dysfunction is Also a Unique Molecular Template used in Nano-electronics, Light Capture Photovoltaics, and Biosensors in Neuromorphic Computing.

Published: June 20, 2024

Amyloid self-assembled from amyloid peptides βΑ40 or βΑ42 is notorious for its neurotoxic effects in plaque and neurofibrillary tangle formations leading to neuron dysfunction diseases of cognitive decline (e.g. Alzheimer’s, Parkinson’s Huntington's disease). This contrasts with so-called functional amyloids which are non-toxic ordered template structures amenable applications tissue engineering. fibrils variable morphology including long hollow fibres flattened tube spiral ribbon-like have been used engineering nano-biology. Protein assemblies based on core display diverse biological functionalities could be applied futuristic self-assembling biomaterials nano-electronics. These possibilities revolutionized the development next generation computers biosensors, ultracapacitors, memristors, actuators, molecular switches also develop artificial synapses. fibril photoelectric photon capture light harvesting technologies innovative nano-photoelectronics photovoltaics. Hybrid Aβ(16–22)-α-synuclein exhibit light-harvesting electron-transfer properties. Engineered thus facilitating advances nano-technology. Furthermore, a better understanding assembly processes it may possible therapeutic methods that prevent toxic build up this polymer brain tissues leads decline. With ever-expanding prevalence these ageing general global population, there certainly clear present need find remedy debilitating conditions.

Language: Английский

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Catalytic Nanomaterials by Conjugation of an Artificial Heme-Peroxidase to Amyloid Fibrils

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(34), P. 45371 - 45382

Published: Aug. 14, 2024

The self-assembly of proteins and peptides into fibrillar amyloid aggregates is a highly promising route to define the next generation functional nanomaterials. Amyloid fibrils, traditionally associated with neurodegenerative diseases, offer exceptional conformational chemical stability mechanical properties, resistance degradation. Here, we report development catalytic nanomaterials through conjugation miniaturized artificial peroxidase (FeMC6*a) self-assembling amyloidogenic peptide derived from human transthyretin, TTR(105-115), whose sequence YTIAALLSPYS. Our synthetic approach relies on fast selective click ligation upon proper modification both FeMC6*a, leading TTRLys

Language: Английский

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Unveiling the multifaceted potential of amyloid fibrils: from pathogenic myths to biotechnological marvels

Biophysical Reviews, Journal Year: 2024, Volume and Issue: 16(6), P. 737 - 751

Published: Sept. 30, 2024

Language: Английский

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SMARTQ: single-molecule amyloid fibRil tracking and quantification. A method for accurately imaging, tracking, and quantifying the growth of individual amyloid fibrils using TIRF

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 145 - 156

Published: Nov. 22, 2024

Language: Английский

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