Caspase-3-Responsive Dual-Enhanced 1H/19F MRI Bimodal Probe for In Vivo Tumor Apoptosis Imaging DOI

Yue Zhu,

Lei Zhang, Sha Li

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 22, 2025

Caspase-3 (Cas-3) is a crucial cysteine protease involved in executing cell apoptosis, process that hallmark of tumor apoptosis response to chemotherapy. Developing molecular imaging probes specifically detect apoptotic signals during treatment considered be one the most important and effective strategies for reducing cancer-associated death rates improving outcomes. However, achieving dual-enhanced bimodal probe single molecule remains significant challenge. In this study, we developed 1H/19F magnetic resonance (MRI) probe, CF3DEVDFFFK(Fmoc)-Gd, responsive caspase-3 vivo cells. Upon interaction with caspase-3, CF3DEVDFFFK(Fmoc)-Gd efficiently splits into two components CF3DEVD FFFK(Fmoc)-C2-Gd, where FFFK(Fmoc)-C2-Gd subsequently self-assembles nanofibers. This activates both 19F MRI 1H MRI, longitudinal relaxivity (r1) increasing from 9.38 ± 0.22 23.24 2.33 mM-1 s-1 at 0.5 T turning on signal due absence paramagnetic relaxation enhancement (PRE) effect. results demonstrated that, after systemic administration, effectively accumulated 4T1 tissues, resulting significantly enhanced visualization activity doxorubicin-treated intensity three times greater than Gd-DTPA. further complemented notable recovery intratumoral injection. These confirm reports through combined offering promising approach preliminary assessment antitumor efficacy vivo.

Language: Английский

Sensitive Multichannel 19F Magnetic Resonance Imaging Enabled by Paramagnetic Fluorinated Ionic Liquid-Based Probes DOI
Limin Chen, Yuhang Jiang,

Nan Xiong

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: 19(9), P. 9061 - 9069

Published: March 1, 2025

Owing to its negligible biological background and high magnetic resonance sensitivity, 19F imaging (MRI) has emerged as a competitive complement for 1H MRI, which is already widely used in biomedical research clinical practice. The performance of MRI greatly reliant on probes, the development poses considerable demands sources. Fluorinated ionic liquids (FILs) have recently attracted increasing attention alternative sources because their good aqueous solubility, ease chemical modification, fluorine contents. However, FIL-based probes significantly restricted by unfavorable relaxation times. Herein, we developed strategy modulate times (including both T1 T2) FILs exploiting paramagnetic enhancement effect Mn2+ ions promote capacity. three including EMIMBF4, BMIMOTf, BMIMPF6 are appropriately tuned with at optimized concentrations, resulting significant signal over 5-fold. We further utilized liposils encapsulate these construct enables fast clear illustrated series vivo experiments. Moreover, made probe containing all concentration, whose capacity multiplexed also validated Our study demonstrates promising potential "hot spot" more importantly, feasibility modulation construction high-performance probes.

Language: Английский

Citations

0
Caspase-3-Responsive Dual-Enhanced 1H/19F MRI Bimodal Probe for In Vivo Tumor Apoptosis Imaging DOI

Yue Zhu,

Lei Zhang, Sha Li

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 22, 2025

Caspase-3 (Cas-3) is a crucial cysteine protease involved in executing cell apoptosis, process that hallmark of tumor apoptosis response to chemotherapy. Developing molecular imaging probes specifically detect apoptotic signals during treatment considered be one the most important and effective strategies for reducing cancer-associated death rates improving outcomes. However, achieving dual-enhanced bimodal probe single molecule remains significant challenge. In this study, we developed 1H/19F magnetic resonance (MRI) probe, CF3DEVDFFFK(Fmoc)-Gd, responsive caspase-3 vivo cells. Upon interaction with caspase-3, CF3DEVDFFFK(Fmoc)-Gd efficiently splits into two components CF3DEVD FFFK(Fmoc)-C2-Gd, where FFFK(Fmoc)-C2-Gd subsequently self-assembles nanofibers. This activates both 19F MRI 1H MRI, longitudinal relaxivity (r1) increasing from 9.38 ± 0.22 23.24 2.33 mM-1 s-1 at 0.5 T turning on signal due absence paramagnetic relaxation enhancement (PRE) effect. results demonstrated that, after systemic administration, effectively accumulated 4T1 tissues, resulting significantly enhanced visualization activity doxorubicin-treated intensity three times greater than Gd-DTPA. further complemented notable recovery intratumoral injection. These confirm reports through combined offering promising approach preliminary assessment antitumor efficacy vivo.

Language: Английский

Citations

0