Current and emerging strategies for the manufacture, implantation, and clinical management of corneal tissue allografts DOI Creative Commons

Jennifer Walshe,

Katrina L. Schmid, Nicholas Toalster

et al.

Clinical and Experimental Optometry, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 12

Published: Dec. 8, 2024

Approximately 40,000 Australians have received a donor corneal tissue transplant over the last 40 years, with primary indications being keratoconus, Fuchs' endothelial dystrophy, bullous keratopathy, and failure of prior transplant. Although cross-linking rigid contact lenses emerged as alternative strategies for management demand corneas is increasing in-line ageing population in Australia. Moreover, owing to lack banking resources less-developed countries, global exceeds supply by 70-fold. These issues, combined evolving surgical techniques, led emergence new storage, processing implantation cells tissues. Organ culture techniques been developed that support storage up 30 days, facilitating improvements surgery scheduling. Bespoke methods are tailored requirements specific conditions, allowing reductions both volume required be transplanted size necessary incision. Further efficiencies patient care may achieved via exploitation cell technologies exemplified through use cultured epithelial treatment limbal stem deficiency. Promising progress has also made developing therapy patients dysfunction. discussed respect their potential impact on clinical presentation who an implant or cells.

Language: Английский

Xenographic lenticule implantation followed by riboflavin and UV treatment: A promising alternative for corneal ectasias management DOI

Fernanda Aparecida Silva Vieira,

Lays Fernanda Nunes Dourado, Thomas Toshio Inoue

et al.

Methods, Journal Year: 2025, Volume and Issue: 234, P. 296 - 304

Published: Jan. 18, 2025

Language: Английский

Citations

1
Advancements in the chemistry of contact Lenses: Innovations and applications DOI Creative Commons

Abdulamier Ahmed Abdulamier,

Lina M. Shaker,

Ahmed A. Al‐Amiery

et al.

Results in Chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 101872 - 101872

Published: Oct. 1, 2024

Language: Английский

Citations

4
Biomaterials for Corneal Regeneration DOI Creative Commons
Yimeng Li, Zhengke Wang

Advanced Science, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 31, 2024

Abstract Corneal blindness is a significant reason for visual impairment globally. Researchers have been investigating several methods corneal regeneration in order to cure these patients. Biomaterials are favored due their biocompatibility and capacity promote cell adhesion. A variety of natural synthetic biomaterials, along with decellularized cornea, employed wound healing. Commonly utilized biomaterials encompass proteins such as collagen, gelatin, silk fibroin (SF), well polysaccharides including alginate, chitosan (CS), hyaluronic acid (HA), cellulose. Synthetic primarily consist polyvinyl alcohol (PVA), poly(ε‐caprolactone) (PCL), poly (lactic‐co‐glycolic acid) (PLGA). Bio‐based materials composites hydrogels, films, scaffolds, patches, nanocapsules, other formats the treatment blinding ocular conditions, wounds, ulcers, endothelium, stromal defects. This review attempts summarize vitro, preclinical, clinical trial studies relevant using within last five years, expect that experiences outcomes will inspire provide practical strategies future development regeneration. Furthermore, potential improvements difficulties discussed.

Language: Английский

Citations

4
Stiffness Regulates the Morphology and Stemness of Limbal Niche Cells Through Unique nYAP/cYAP Translocation DOI Creative Commons
Xiao Zhou,

Lingjuan Xu,

Yongyao Tan

et al.

Investigative Ophthalmology & Visual Science, Journal Year: 2025, Volume and Issue: 66(2), P. 43 - 43

Published: Feb. 14, 2025

Purpose: To investigate the effect of matrix stiffness on morphology and stem characters maintenance differentiation limbal niche cells (LNCs) mechanisms involved. Methods: Human LNCs were isolated, cultured, identified based published literature, from passages 4 to 6 (P4–P6) used in this study. They coated with hydrogels different concentrations prepare matrices stiffnesses, non-coated plate for control group. Elastic modulus values determined by atomic force microscopy (AFM). The expression putative cell markers (SOX2, OCT4, PAX6) fibrosis (α-SMA, COL1A1, S100A4) was analyzed immunofluorescence quantitative reverse-transcription PCR (RT-qPCR). intracellular distribution Yes-associated protein (YAP) drosophila mothers against decapentaplegic family members 2 3 (SMAD2/3) accordingly using western blot. Results: elastic plastic, low-concentration hydrogel–coated surfaces, high-concentration surfaces 3261.05 ± 172.78 MPa, 30.39 5.84 kPa, 6.99 4.04 respectively; thus, they referred as dish, stiff, soft groups. Using an vitro model explore LNCs, we found that a substrate could activate YAP change elevate stemness whereas activation SMAD2/3 stiff decreased nuclear (nYAP) levels, leading myofibroblast phenotype. Inhibition substrates partially restored LNC promoting translocation. Conclusions: Our findings confirm regulates through YAP/SMAD signaling pathway, indicating potential strategy treatment deficiency LNCs.

Language: Английский

Citations

0
3D mesenchymal stem cell exosome-functionalized hydrogels for corneal wound healing DOI

Yuehe Xu,

Chao Wei,

Li Ma

et al.

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: 380, P. 630 - 646

Published: Feb. 16, 2025

Language: Английский

Citations

0
Understanding the role of electrostatic force, van der Waals force, and osmotic pressure in retinal function and barrier integrity DOI Creative Commons
Khayry Al‐Shami,

Jafar Shatnawi,

Khaled Qasagsah

et al.

International Journal of Retina and Vitreous, Journal Year: 2025, Volume and Issue: 11(1)

Published: Feb. 19, 2025

Abstract The retina’s intricate interplay of forces and structures, with a focus on the retinal pigment epithelium (RPE) photoreceptors, is essential for health function. Among these forces, electrostatic play crucial role, working alongside van der Waals oncotic pressure to maintain attachment RPE ensure integrity blood-retina barrier (BRB). composition interphotoreceptor matrix (IPM), influenced by molecules like Retbindin secreted rod further modulates affecting processes visual regeneration metabolite exchange. In context tissue engineering new technologies support cells-based treatments, are harnessed optimize nutrient supply transplanted cells reducing pore size in electrospun polymer membranes. Scaffold-based strategies repair also utilize electrostatic, hydrophobic, Waals, hydrogen bonding enhance cell adhesion growth, mimicking basement membrane. Understanding complex dynamics retinal-RPE interactions holds promise innovative treatments disorders, emphasizing balance between pressure, more. These insights open exciting avenues research therapeutic interventions ophthalmology. Additionally, explored adhesion, their potential role discussed, particularly relation melanin’s protective properties against blue light-induced damage. Tissue approaches, both scaffold-free scaffold-based, highlighting importance physical surface adhesive preserving engineered tissue. Overall, this abstract provides comprehensive overview multifaceted other biology implications future clinical applications

Language: Английский

Citations

0
Limbal stem cell deficiency approaches and limbal niche restoration DOI Creative Commons

Zahra Bibak-Bejandi,

Zohre Arabpour,

Alireza Razavi

et al.

Indian Journal of Ophthalmology, Journal Year: 2025, Volume and Issue: 73(4), P. 468 - 482

Published: March 27, 2025

Approaches to limbal stem cell deficiency remain challenging, especially in bilateral cases, where healthy cells are not accessible. While living-related allogeneic and sources have been utilized, their dependence on immunosuppression its associated side effects pose significant limitations. Mucosal mesenchymal shown potential for differentiation into promoting corneal healing, primarily when cultured the amniotic membrane or fibrin. However, none can fully replicate original limbus. Innovations surgical techniques, such as simple oral mucosal transplantation subconjunctival intrastromal injections, emerging approaches. For successful regeneration, both appropriate suitable scaffolds essential. Recent studies decellularized acellularized limbus models demonstrated provide a three-dimensional native structure seeding, retention, differentiation. Creating thin, evenly scaffold is critical step ensuring proper corneo-limbal slope formation, facilitating migration ocular surface. Harvesting limbus, decellularization, seeding three main steps reconstruction. focus microkeratome-assisted harvesting create even, 360-degree graft. This technique helps form an attached interface, migration. In second step, acellularization performed preserve extracellular matrix much possible, maintaining hemostasis supporting paracrine interactions. The final involve recellularization onto eye. We summarize various decellularization methods, outcomes, More clinical needed validate this phase of treatment.

Language: Английский

Citations

0
Development of a Microscopy-Based Diagnostic Test for Alkali Injury-Induced Limbal Stem Cell Deficiency Through Autofluorescence Multispectral Imaging DOI

Xiaohu Xu,

Lina Sprogyte,

Alexander Richardson

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Tissue Engineering Strategies for Ocular Regeneration; from Bench to the Bedside DOI Creative Commons
Zekra Mousavi, Masood Bagheri, Gelavizh Rostaminasab

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(20), P. e39398 - e39398

Published: Oct. 1, 2024

Language: Английский

Citations

2
Current and emerging strategies for the manufacture, implantation, and clinical management of corneal tissue allografts DOI Creative Commons

Jennifer Walshe,

Katrina L. Schmid, Nicholas Toalster

et al.

Clinical and Experimental Optometry, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 12

Published: Dec. 8, 2024

Approximately 40,000 Australians have received a donor corneal tissue transplant over the last 40 years, with primary indications being keratoconus, Fuchs' endothelial dystrophy, bullous keratopathy, and failure of prior transplant. Although cross-linking rigid contact lenses emerged as alternative strategies for management demand corneas is increasing in-line ageing population in Australia. Moreover, owing to lack banking resources less-developed countries, global exceeds supply by 70-fold. These issues, combined evolving surgical techniques, led emergence new storage, processing implantation cells tissues. Organ culture techniques been developed that support storage up 30 days, facilitating improvements surgery scheduling. Bespoke methods are tailored requirements specific conditions, allowing reductions both volume required be transplanted size necessary incision. Further efficiencies patient care may achieved via exploitation cell technologies exemplified through use cultured epithelial treatment limbal stem deficiency. Promising progress has also made developing therapy patients dysfunction. discussed respect their potential impact on clinical presentation who an implant or cells.

Language: Английский

Citations

0