A Stemness‐Enhanced Eye Drop Stimulates Corneal Regeneration via Sustained Release of Versican and Providing Lubricated Substrate DOI Open Access

Shiding Li,

Siyi Zhang, Fei Fang

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 23, 2024

Abstract Stem cells are vital for tissue regeneration, and their dysfunction can lead to diseases like aplastic anemia Alzheimer's disease. Limbal epithelial stem (LESCs) maintain corneal transparency as the sole source of epithelium. Dysfunction or deficiency LESCs causes opacity, neovascularization blindness, known limbal cell (LSCD). Current LSCD treatment mainly relies on transplantation, which faces challenges limited sources immune rejection, making therapy challenging often ineffective. This study introduces a cell‐free, stemness‐enhanced eye drop (SEED @ ) stimulate autologous regeneration. SEED , thermosensitive Pluronic F‐127 hydrogel loaded with versican, promote proliferation, chemotaxis, migration while maintaining stemness via Wnt/β‐catenin signaling pathway. In vivo, alone lubricate cornea repair in mouse alkali burn model. addition, forms thin, transparent layer ocular surface, enabling sustained versican release up 24 h. Notably, twice‐daily application significantly accelerated regeneration rabbit model, achieving approximately 95% by day 14. Collectively, shows great potential treating offers promising drug delivery platform diseases.

Language: Английский

Innate immune cells in vascular lesions: mechanism and significance of diversified immune regulation DOI Creative Commons
Jinjing Wu, Y. Qian,

Yang Kuang

et al.

Annals of Medicine, Journal Year: 2025, Volume and Issue: 57(1)

Published: Jan. 23, 2025

Angiogenesis is a complex physiological process. In recent years, the immune regulation of angiogenesis has received increasing attention, and innate cells, which are centred on macrophages, thought to play important roles in vascular neogenesis development. Various cells can act vasculature through variety mechanisms, with commonalities as well differences synergistic effects, crucial for progression lesions. monotherapy antiangiogenic drugs encountered therapeutic bottlenecks because short-term effect 'vascular normalization'. The combination treatment therapy immunotherapy breaks traditional pattern. While it remarkable curative survival benefits, also faces many challenges. This review focuses mainly introduces regulatory mechanisms monocytes, natural killer (NK) dendritic (DCs) neutrophils purpose this paper was elucidate underlying development current research status regulating lesions different states. provides theoretical basis addressing aberrant disease processes or finding new targets inflammation tumor.

Language: Английский

Citations

0
Selective mutation of the tumor cells Redirects virus-specific CD8 + T cells for suppressing post-operative TNBC recurrence DOI

Shizhen Geng,

Yunya Zhang, Danyu Wang

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 160636 - 160636

Published: Feb. 1, 2025

Language: Английский

Citations

0
Extracellular vesicle as a next-generation drug delivery platform for rheumatoid arthritis therapy DOI
Yi Jin, Cong Xu, Yujuan Zhu

et al.

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: 381, P. 113610 - 113610

Published: March 7, 2025

Language: Английский

Citations

0
The Role of Non-coding RNAs in Diabetic Retinopathy: Mechanistic Insights and Therapeutic Potential DOI

Zhuan Zuo,

Ni Li, Qian Zhang

et al.

Molecular Neurobiology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Exosomes in treating Eye Diseases: Targeting strategies DOI

Pengfei Ge,

Jingfan Wang, Ping Xie

et al.

Life Sciences, Journal Year: 2025, Volume and Issue: unknown, P. 123598 - 123598

Published: March 1, 2025

Language: Английский

Citations

0
Mesenchymal stem cell-derived exosomes: an emerging therapeutic strategy for hepatic ischemia-reperfusion injury DOI Creative Commons
Bo Zhao, Jianshu Wei,

Zijian Jiang

et al.

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 14, 2025

Hepatic ischemia-reperfusion injury (HIRI) severely threatens the success rates of liver surgery and transplantation. Its complex pathological process involves multiple factors such as oxidative stress, inflammatory responses, ferroptosis, creating an urgent need for new therapeutic strategies. Exosomes derived from mesenchymal stem cells (MSCs) are emerging a next-generation acellular approach. With their outstanding immune-regulatory capabilities, significant reparative functions, good biocompatibility, they leading innovations in field HIRI treatment. This article provides systematic comparison characteristics MSC-derived exosomes(MSC-EXOs) four different sources: adipose tissue, bone marrow, umbilical cord, induced pluripotent cells. Although clinical translation MSC-EXOs still faces challenges variations isolation methods, large-scale production, safety assessments, remarkable effects vast application potential signal arrival era precision treatment HIRI. review not only comprehensive theoretical foundation to promote but also opens up innovative research directions regenerative medicine.

Language: Английский

Citations

0
Advancements in plant-derived exosome-like vesicles: Versatile bioactive carriers for targeted drug delivery systems DOI Creative Commons

Haixia Shen,

Shuaiguang Li, Liyuan Lin

et al.

Journal of Pharmaceutical Analysis, Journal Year: 2025, Volume and Issue: unknown, P. 101300 - 101300

Published: April 1, 2025

Language: Английский

Citations

0
In Situ, Fusion-Free, Multiplexed Detection of Small Extracellular Vesicle miRNAs for Cancer Diagnostics DOI
Fei Zhou, Pan Li, Xiaowei Ma

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 19, 2024

Tumor-derived small extracellular vesicle (sEV) microRNAs (miRNAs) are emerging biomarkers for cancer diagnostics. Conventional sEV miRNA detection methods necessitate the lysis of sEVs, rendering them laborious and time-consuming potentially leading to damage or loss miRNAs. Membrane fusion-based

Language: Английский

Citations

2
Trivalent Disulfide Unit-Masked System Efficiently Delivers Large Oligonucleotide DOI Creative Commons
Lei Wang, Xiao Liu,

Yiliang Wu

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(17), P. 4223 - 4223

Published: Sept. 5, 2024

Oligonucleotide drugs are shining in clinical therapeutics, but efficient and safe delivery systems severely limit their widespread use. A disulfide unit technology platform based on dynamic thiol exchange chemistry at the cell membrane has potential for drug delivery. However, alteration of CSSC dihedral angle induced by different substituents directly affects effectiveness this its stability. Previously, we constructed a trivalent low that can effectively promote cellular uptake small molecules. Here, novel unit-masked oligonucleotide hybrid unit, motivated prodrug design. Cellular imaging results showed such system exhibited superior efficiency than commercial Lipo2000 without cytotoxicity. The reagents significantly reduced (57–74%), which proved to be endocytosis-independent. In addition, vivo distribution experiments mice rapidly distributed liver tissues with duration action more 24 h, representing means silencing genes involved pathogenesis liver-like diseases. conclusion, deliver large drugs.

Language: Английский

Citations

0
Engineered Extracellular Vesicles as a New Class of Nanomedicine DOI Creative Commons

Xiaowei Wen,

Zhengping Hao, Haofan Yin

et al.

Chem & Bio Engineering, Journal Year: 2024, Volume and Issue: 2(1), P. 3 - 22

Published: Oct. 28, 2024

Extracellular vesicles (EVs) are secreted from biological cells and contain many molecules with diagnostic values or therapeutic functions. There has been great interest in academic industrial communities to utilize EVs as tools for diagnosis therapeutics. In addition, can also serve delivery vehicles molecules. An indicator of the enormous is large number review articles published on EVs, focus ranging their biology applications. emerging trend EV research produce "engineered EVs", which essentially enhanced version EVs. engineering be conducted by cell culture condition control, genetic engineering, chemical engineering. Given nanometer-scale sizes potentials, engineered an class nanomedicines. So far, overwhelming majority preclinical studies; there only a very small reported clinical trials. This Review focuses more specific being applications The various approaches producing diseases reviewed. Furthermore, vivo imaging mechanistic understandings, translation aspects discussed. discussion primarily studies while briefly mentioning With continued interdisciplinary efforts biologists, pharmacists, physicians, bioengineers, engineers, could become powerful solution major such neurological, immunological, cardiovascular diseases.

Language: Английский

Citations

0