Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 271, P. 117069 - 117069
Published: Dec. 20, 2024
Language: Английский
Comprehensive Reviews in Food Science and Food Safety, Journal Year: 2025, Volume and Issue: 24(2)
Published: March 1, 2025
Abstract Pickering emulsions (PEs) have attracted considerable interest as platforms for encapsulating and controlling the release of bioactive compounds. Recent studies emphasize potential soybean protein nanoparticles to improve PE‐based carriers, enhancing stability bioavailability these compounds through unique self‐assembly behaviors. This review analyzes recent advancements in use nanoparticle‐stabilized PEs carriers Various fabrication techniques, including physical, chemical, biological methods, are explored. The effectiveness nanoparticles, both individually combination with polysaccharides or polyphenols, is evaluated, highlighting their roles stabilizing functionality. Findings indicate that effective stabilizers a wide range PE structures, oil‐in‐water, water‐in‐oil, high internal phase PEs, emulgels. Fabrication properties particles, processing parameters, formulations significantly influence interfacial behavior, structure, functionality PEs. Additionally, innovative applications future developments protein–based discussed, emphasizing plant‐based substitutes advanced materials. Despite extensive discussions on various food forms, research into techno‐functional flavor mechanisms remains limited.
Language: Английский
Citations
1
Molecules, Journal Year: 2025, Volume and Issue: 30(3), P. 439 - 439
Published: Jan. 21, 2025
HUG is the HELP-UnaG recombinant fusion protein featuring typical functions of both HELP and UnaG. In HUG, domain a thermoresponsive human elastin-like polypeptide. It forms shield enwrapping UnaG that emits bilirubin-dependent fluorescence. Here, we recapitulate technological development this bifunctional synthetic from theoretical background its distinct moieties to detailed characterization macromolecular functional properties. These pieces knowledge are foundations for production application in fluorometric analysis bilirubin congeners, biliverdin glucuronide. bile pigments metabolites arise catabolism heme, prosthetic group cytochromes, hemoglobin several other intracellular enzymes engaged electron transfer, oxygen transport protection against free radicals. The assay powerful, user-friendly affordable analytical tool alone supports research at each level complexity or taxonomy living entities, enzymology, cell biology pathophysiology veterinary clinical sciences.
Language: Английский
Citations
0
The Chemical Record, Journal Year: 2025, Volume and Issue: unknown
Published: April 10, 2025
Abstract Mimicking natural systems, self‐assembly has been employed for constructing highly stable and well‐ordered supramolecular structures. Amino acids are frequently used as building blocks in the process due to their advantageous characteristics including easy availability, functionalization, tunable mechanical property, biodegradability. In situ generation of active obtain complex materials via enhanced application biomedical fields bio‐imaging, therapeutics. Single amino acid small building‐block can provide artificial with unique properties. this review, we summarize tyrosine‐derivatives single acid‐based providing assemblies perspectives on potential impact. Finally, discuss ongoing challenges future research.
Language: Английский
Citations
0
Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101476 - 101476
Published: Jan. 9, 2025
Stem cell-based therapy has emerged as a promising approach for heart repair, potentially regenerating damaged tissue and improving outcomes patients with disease. However, the efficacy of stem therapies remains limited by several challenges, including poor cell survival, low retention rates, integration, functional outcomes. This article reviews current enhancement strategies to optimize mesenchymal cardiac repair. Key approaches include optimizing delivery methods, enhancing engraftment, promoting functions through genetic molecular modifications, paracrine effects cells, leveraging biomaterials engineering techniques. By focusing on these techniques, paper highlights innovative that can transform into more viable effective treatment option The ongoing research technological advancements continue push boundaries, hoping make mainstream
Language: Английский
Citations
0
Advanced Materials Technologies, Journal Year: 2025, Volume and Issue: unknown
Published: March 18, 2025
Abstract Implantable medical devices must meet biocompatibility requirements before clinical use, making the profiling of extractables and leachables crucial for assessing risks. Although guidelines outline methods identifying extractables, they lack guidance on monitoring leachables. This study evaluates stability a brain phantom stored at 37 °C 180 days monitoring. White matter (WM) gray (GM) components, creating an emulsion‐gel with cholesterol, agar, carboxymethylcellulose, are separated. Homogeneity is assessed using polydispersity index (PDI), investigated Young's modulus (YM), rheological behavior, apparent diffusion coefficient water (ADCs) immediately after preparation storage. PDI values 0.258 ± 0.009 WM 0.362 0.008 GM. YM 2.32 0.14 kPa 1.11 0.11 Dynamic viscosities 16.62 6.36 s 5.99 2.79 GM 0.1 rad −1 . ADCs 1130 64 µm 2 1449 34 All parameters remain within physiological range during °C. Comparison data necessary to validate this medium leachable studies.
Language: Английский
Citations
0
Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown
Published: April 4, 2025
Abstract Biomimetic materials hold significant potential for a wide range of applications, yet developing straightforward and versatile methods to create muscle‐mimetic, high‐performance protein with anisotropic properties remains major challenge. In this study, simple general strategy is presented alignment driven by directional airflow, enabling the design printable, muscle‐mimetic proteins. By utilizing airflow during hydrogel drying in combination rapid photochemistry, molecules align efficiently single direction. Similar natural muscle, mechanical these can be further enhanced through training, achieving remarkable strength up ≈8 MPa at 600% strain an anisotropy factor 3.0. This fabrication process compatible conventional printing techniques, allowing creation complex structures controlled tailored properties. Notably, exhibit biomimetic actuation (≈6 s) adaptability under physiological conditions. Their demonstrated proof‐of‐concept applications as artificial grippers vessel dilators, which remain stable months but degrade rapidly within hours after enzymatic treatment post‐therapy. directional‐airflow‐driven method, along resulting materials, offers promising implications fields, from medical devices adaptive technologies.
Language: Английский
Citations
0
ACS Macro Letters, Journal Year: 2025, Volume and Issue: unknown, P. 679 - 686
Published: May 9, 2025
Peptide self-assembly has been used to fabricate synthetic hydrogels that emulate many of the chemical and physical properties natural hydrogels. However, these materials often lack stability for applications do not display native bioactivity found in tissue. Here we demonstrate a hybrid hydrogel system which self-assembling peptides are integrated with polysaccharides enhance gelation provide improved mechanics bioactivity. A peptide based on tryptophan zipper (trpzip) motif was mixed anionic polysaccharide gellan gum, demonstrating within minutes increased stiffness compared trpzip alone. The material maintained viscoelastic character shear-thinning, self-healing, stress-relaxation order like collagen. All supported cell adhesion viability gum content, promoting assembly into aggregates. enhanced kinetics, stability, make them promising candidates as matrices culture reagents biofabrication syringe extrusion biological delivery.
Language: Английский
Citations
0
Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 378, P. 460 - 475
Published: Dec. 21, 2024
Language: Английский
Citations
1
ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 16, 2024
The biofabrication of recombinant structural proteins with a range mechanical or features usually relies on the generation protein libraries displaying variations in terms amino acid composition, block structure, molecular weight, physical/chemical cross-linking sites. This approach, while highly successful generating wealth knowledge regarding links between design and material properties, has some inherent limitations related to its low throughput. slows down pace development de novo proteins. Here, we propose an approach tune viscoelastic properties temperature-responsive hydrogels made silk-elastin-like polypeptides (SELPs) without modifying their sequence. To do so, subject purified SELPs two different postprocessing methods─water annealing EtOH annealing─that alter topology disordered SELP networks via formation ordered intermolecular β-sheet physical cross-links. Combining analytical techniques, connect order/disorder balance gelling behavior. Furthermore, show that introducing functional (in this case, biomineralizing peptide) sequence can disrupt self-assembly such disruption only be overcome by annealing. Our results suggest as-purified might simple into biomaterials bespoke beyond traditional developing genetic engineering.
Language: Английский
Citations
0
Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 271, P. 117069 - 117069
Published: Dec. 20, 2024
Language: Английский
Citations
0