3D bioprinted thick hepatic constructs with vascular network as a physiologically relevant in vitro organ model DOI Creative Commons

Young-Wook Moon,

Timothy Dobroski,

Kelsey Willson

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: 32, P. 101786 - 101786

Published: April 21, 2025

Establishing adequate vascularization to engineered organs remains a significant challenge that must be addressed. This study presents novel approach fabricating viable thick metabolic tissue (>1 cm3) for applications in human physiology, fundamental biology, and medicine. We designed construct with gyroid-shaped architecture enable uniform flow surface shear stress adequately covers the inner surfaces of cell-laden constructs. The constructs (1 × 1 were fabricated using digital light projection (DLP) printer poly(ethylene glycol) (PEG)/gelatin methacryloyl (GelMA) bioink combined hepatocytes (HepG2), followed by coating interconnected vascular channels endothelial cells (ECs). These then placed chambers connected medium reservoir continuous perfusion up 30 days. retained their original dimensions, maintained greater than 85 % viability at all time points. Immunofluorescent staining confirmed ECs cell-specific markers (HNF4-α/albumin vWF ECs). EC layer effectively lined lumens, while hepatocyte aggregates populated interior Functional assays demonstrated produced albumin bilirubin levels comparable those observed humans, validating functionality hepatic successfully developed thick, vascularized an vitro environment, maintaining native liver over innovative gyroid design applied these organ represents advancement developing physiologically relevant models.

Language: Английский

Advances and Challenges in 3D Bioprinted Cancer Models: Opportunities for Personalized Medicine and Tissue Engineering DOI Open Access
Sai Liu, Pan Jin

Polymers, Journal Year: 2025, Volume and Issue: 17(7), P. 948 - 948

Published: March 31, 2025

Cancer is the second leading cause of death worldwide, after cardiovascular disease, claiming not only a staggering number lives but also causing considerable health and economic devastation, particularly in less-developed countries. Therapeutic interventions are impeded by differences patient-to-patient responses to anti-cancer drugs. A personalized medicine approach crucial for treating specific patient groups includes using molecular genetic screens find appropriate stratifications patients who will respond (and those not) treatment regimens. However, information on which risk stratification method can be used hone cancer types likely responders agent remains elusive most cancers. Novel developments 3D bioprinting technology have been widely applied recreate relevant bioengineered tumor organotypic structures capable mimicking human tissue microenvironment or adequate drug high-throughput screening settings. Parts autogenously printed form tissues computer-aided design concept where multiple layers include different cell compatible biomaterials build configurations. Patient-derived stromal cells, together with material, extracellular matrix proteins, growth factors, create bioprinted models that provide possible platform new therapies advance. Both natural synthetic biopolymers encourage cells biological materials models/implants. These may facilitate physiologically cell-cell cell-matrix interactions heterogeneity resembling real tumors.

Language: Английский

Citations

0
Unlocking the Human Blood-brain barrier (BBB) Characteristics for the Development of Nano-Delivery Strategies for Central Nervous System Therapies DOI
Cemile Gokce, Bağdagül Altun,

Esen Kirit

et al.

Journal of Drug Delivery Science and Technology, Journal Year: 2025, Volume and Issue: unknown, P. 106961 - 106961

Published: April 1, 2025

Language: Английский

Citations

0
The Future of Automated Tissue Engineering: Robotic‐Assisted Strategies for Complex 3D Tissue Bottom‐Up Assembly DOI
Ana Margarida Almeida,

João Mazeda,

Ana Rita Pinho

et al.

Advanced Materials Technologies, Journal Year: 2025, Volume and Issue: unknown

Published: May 4, 2025

Abstract Over the years, need for novel solutions to replace damaged tissues has led development of new tissue‐engineering strategies. Bottom‐up approaches have gained interest mimicking hierarchical cellular organization and intricate nature tissues. Among these approaches, automated‐assisted techniques, such as robotic handling, potential precisely control spatial building blocks, allowing creation highly specific functional Recognizing handling in tissue engineering, this review provides an overview robot‐assisted bottom‐up engineering complex tissues, highlighting advantages limitations various systems currently being explored. To address growing field, also discusses key considerations assembly living while providing insights into future directions challenges rapidly evolving field.

Language: Английский

Citations

0
Assessing the landscape of clinical and observational trials involving bioprinting: a scoping review DOI Creative Commons
Yumi L. Briones, Beatrice Paula G. Pascua, Narra Maria J. Tiangco

et al.

3D Printing in Medicine, Journal Year: 2025, Volume and Issue: 11(1)

Published: Feb. 17, 2025

Bioprinting is a tissue engineering technique that rapidly evolving to include complex clinical applications. However, there limited evidence describing how far bioprinting has progressed past the pre-clinical stage. Thus, we conducted scoping review assess landscape of studies, including interventional and observational trials, involving by charting trends in general characteristics, application, trial design. The term "bioprint" its variants were searched five databases (ICTRP, ScanMedicine, CENTRAL, NIHCC, HCCTD) two registries (ClinicalTrials.gov, PHRR) on 22 February 2024. This was followed duplicate removal dual independent finalize inclusion list. We included trials published or translated English mentioning their design, while excluded those did not adhere our definition bioprinting. Finally, data charted synthesized narratively. Of 36 total search records, 11 met criteria. Registration dates ranged from 2016 2023, with China conducting most globally. Four had results, remaining still progress. aimed implant bioprinted tissues made autologous cells, blood vessels, trachea, external ear, wound dressings. other seven studies aiming bioprint cell-laden vitro models study conditions such as cancer. early stages research, focus producing patient-specific for cancer precision medicine regenerative purposes. More standardized reporting bioprinting-related information needed improve research transparency replicability. As body grows, may be used framework monitor translation over years.

Language: Английский

Citations

0
Nanocomposite Hydrogels and Micro/Nanostructures for Printing Organoids DOI

Daixi Xie,

Bingda Chen,

Wenqing Wang

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: March 31, 2025

Organoids are 3D artificial miniature organs composed of a cluster self-renewing and self-organizing cells in vitro, which mimic the functions real organs. Nanotechnologies, including preparation nanomaterials fabrication micro/nanostructures, have been proven to promote cell proliferation, guide differentiation, regulate self-organization, showing great promise engineering organoids. In this Perspective, different types nanocomposite hydrogels for organoid culture summarized, effects micro/nanostructures on growth development discussed, bioprinting technologies constructing models introduced.

Language: Английский

Citations

0
Editorial: Biomaterials and biological regulation for bone tissue remodeling and regeneration DOI Creative Commons
Xianrui Yang, Masashi Nagao, Chenhe Zhou

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: April 2, 2025

Language: Английский

Citations

0
Human Extracellular Matrix‐Like Collagen‐Based Hydrogels for Soft Tissue Regeneration and Mandibular Retrognathia Treatment DOI
E. Xiao, Peng Wang, Kaikai Zheng

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 8, 2025

Abstract Skin aging, trauma, and congenital diseases lead to tissue defects functional loss. Effective regeneration remains challenging due limited material bioactivity inadequate consideration of mechanical cues. This study develops a hydrogel integrating human extracellular matrix‐like collagen (hCol) derived from induced adipose mesenchymal stem cells (hASCs) with hyaluronic acid (HA) polyethylene glycol diamine (PEGDA), engineered achieve modulus (≈1 kPa) representative subcutaneous soft tissue. The hCol, produced at scale, provides essential biochemical signals, which, in conjunction the properties, synergistically modulate immune responses, cellular differentiation, anti‐aging processes. Both animal experiments clinical trials validate regenerative efficacy hydrogels. Clinical therapy for mandibular retrognathia shows rapid sustained improvements, repair rates 95.2% 3 months 76.2% 6 months. These findings underscore hydrogel's ability reshape microenvironment, supporting immediate regeneration, while offering promising platform future biomaterial development engineering.

Language: Английский

Citations

0
Recent Trends and Future Directions in 3D Printing of Biocompatible Polymers DOI Creative Commons
Maryam Aftab, S. Ikram, Muneeb Ullah

et al.

Journal of Manufacturing and Materials Processing, Journal Year: 2025, Volume and Issue: 9(4), P. 129 - 129

Published: April 14, 2025

Three-dimensional (3D) bioprinting using biocompatible polymers has emerged as a revolutionary technique in tissue engineering and regenerative medicine. These biopolymers mimic the extracellular matrix (ECM) enhance cellular behavior. The current review presents recent advancements additive manufacturing processes including Stereolithography (SLA), Fused Filament Fabrication (FFF), Selective Laser Sintering (SLS), inkjet printing. It also explores fundamentals of 3D printing properties for bioprinting. By mixing biopolymers, enhancing rheological characteristics, adding bioactive components, further have been made organ transplantation, drug development, engineering. As research progresses, potential to fundamentally transform healthcare system is becoming obvious clear. However, therapeutic printed structures hindered by issues such material anisotropy, poor mechanical properties, need more biodegradable architectures. Future should concentrate on optimizing process sophisticated computational techniques, systematically examining characteristics customizing bioinks different cell types, exploring sustainable materials.

Language: Английский

Citations

0
3D bioprinted thick hepatic constructs with vascular network as a physiologically relevant in vitro organ model DOI Creative Commons

Young-Wook Moon,

Timothy Dobroski,

Kelsey Willson

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: 32, P. 101786 - 101786

Published: April 21, 2025

Establishing adequate vascularization to engineered organs remains a significant challenge that must be addressed. This study presents novel approach fabricating viable thick metabolic tissue (>1 cm3) for applications in human physiology, fundamental biology, and medicine. We designed construct with gyroid-shaped architecture enable uniform flow surface shear stress adequately covers the inner surfaces of cell-laden constructs. The constructs (1 × 1 were fabricated using digital light projection (DLP) printer poly(ethylene glycol) (PEG)/gelatin methacryloyl (GelMA) bioink combined hepatocytes (HepG2), followed by coating interconnected vascular channels endothelial cells (ECs). These then placed chambers connected medium reservoir continuous perfusion up 30 days. retained their original dimensions, maintained greater than 85 % viability at all time points. Immunofluorescent staining confirmed ECs cell-specific markers (HNF4-α/albumin vWF ECs). EC layer effectively lined lumens, while hepatocyte aggregates populated interior Functional assays demonstrated produced albumin bilirubin levels comparable those observed humans, validating functionality hepatic successfully developed thick, vascularized an vitro environment, maintaining native liver over innovative gyroid design applied these organ represents advancement developing physiologically relevant models.

Language: Английский

Citations

0