Multi-omics pan-cancer analysis reveals the diagnostic and prognostic value of C8orf76, with experimental validation of its impact on lung adenocarcinoma cell proliferation DOI Creative Commons

Xiaohong Zhong,

Zhiyong Zhang,

Rongjing Gao

et al.

Frontiers in Genetics, Journal Year: 2025, Volume and Issue: 16

Published: March 26, 2025

Background Chromosome 8 open reading frame 76 (C8orf76) is a nuclear protein-encoding gene, has received limited attention in current study. Multi-omics pan-cancer analysis focused on the diagnosis, prognosis, immune cell infiltration, methylation, and anti-cancer drug sensitivity remains an enigma. The effect of C8orf76 lung adenocarcinoma (LUAD) unknown. Methods by utilizing datasets including UALCAN, TIMER 2.0, Human Protein Atlas (HPA), Cancer Genome (TCGA), Genotype-Tissue Expression (GTEx), cBioPortal, Gene Profiling Interactive Analysis (GEPIA), OncoDB, MethSurv datasets, were conducted to analyze across 33 cancer types. Furthermore, differential R packages uesd for in-depth C8orf76. correlation between expression diagnostic, genetic alteration, mRNA modification, DNA lncRNA-miRNA-C8orf76 regulatory network, anti-tumor drugs response explored evaluate potential roles Most importantly, experiments quantitative polymerase chain reaction (qPCR), RNA interference (RNAi), Western blotting (WB), Edu staining, performed experimental verification. Results It was noted that markedly elevated multiple tumor Moreover, showed as diagnostic prognostic biomarker. Besides, it confirmed related infiltration cells. network established study LUAD. Experimental validation LUAD A549 cells demonstrated knockdown significantly inhibited proliferation Conclusion present first report multi-omics predicts promising target immunology, chemotherapy, highlighting its influence with validation.

Language: Английский

Innovative Nanomedicine Delivery: Targeting Tumor Microenvironment to Defeat Drug Resistance DOI Creative Commons
Wenjun Meng, Li Huang,

Jia‐Min Guo

et al.

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(12), P. 1549 - 1549

Published: Dec. 3, 2024

Nanodrug delivery systems have revolutionized tumor therapy like never before. By overcoming the complexity of microenvironment (TME) and bypassing drug resistance mechanisms, nanotechnology has shown great potential to improve efficacy reduce toxic side effects. This review examines impact TME on recent advances in nanomedicine overcome this challenge. Characteristics such as hypoxia, acidity, high interstitial pressure significantly effectiveness chemotherapy radiotherapy, leading increased cells. Then, summarizes innovative nanocarrier designs for these microenvironmental features, including hypoxia-sensitive nanoparticles, pH-responsive carriers, multifunctional nanosystems that enable targeted release improved penetration accumulation tumors. combining with therapeutic strategies, offers a novel perspective by focusing design nanocarriers interact TME, dimension often overlooked similar reviews. We highlight dual role modulation, emphasize their resistance, look at future research directions.

Language: Английский

Citations

3
Multi-omics pan-cancer analysis reveals the diagnostic and prognostic value of C8orf76, with experimental validation of its impact on lung adenocarcinoma cell proliferation DOI Creative Commons

Xiaohong Zhong,

Zhiyong Zhang,

Rongjing Gao

et al.

Frontiers in Genetics, Journal Year: 2025, Volume and Issue: 16

Published: March 26, 2025

Background Chromosome 8 open reading frame 76 (C8orf76) is a nuclear protein-encoding gene, has received limited attention in current study. Multi-omics pan-cancer analysis focused on the diagnosis, prognosis, immune cell infiltration, methylation, and anti-cancer drug sensitivity remains an enigma. The effect of C8orf76 lung adenocarcinoma (LUAD) unknown. Methods by utilizing datasets including UALCAN, TIMER 2.0, Human Protein Atlas (HPA), Cancer Genome (TCGA), Genotype-Tissue Expression (GTEx), cBioPortal, Gene Profiling Interactive Analysis (GEPIA), OncoDB, MethSurv datasets, were conducted to analyze across 33 cancer types. Furthermore, differential R packages uesd for in-depth C8orf76. correlation between expression diagnostic, genetic alteration, mRNA modification, DNA lncRNA-miRNA-C8orf76 regulatory network, anti-tumor drugs response explored evaluate potential roles Most importantly, experiments quantitative polymerase chain reaction (qPCR), RNA interference (RNAi), Western blotting (WB), Edu staining, performed experimental verification. Results It was noted that markedly elevated multiple tumor Moreover, showed as diagnostic prognostic biomarker. Besides, it confirmed related infiltration cells. network established study LUAD. Experimental validation LUAD A549 cells demonstrated knockdown significantly inhibited proliferation Conclusion present first report multi-omics predicts promising target immunology, chemotherapy, highlighting its influence with validation.

Language: Английский

Citations

0