Total cardiolipin levels in gastric and colon cancer: evaluating the prognostic potential DOI Creative Commons
Pāvels Dimitrijevs,

Ilona Freiliba,

Andrejs Pčolkins

et al.

Lipids in Health and Disease, Journal Year: 2025, Volume and Issue: 24(1)

Published: Feb. 27, 2025

Cardiolipin (CL) is a signature phospholipid of mitochondria that maintains the integrity mitochondrial membrane and supports proper function. Alterations in CL level composition can impair or, conversely, improve function bioenergetics, both which are critical for cancer metabolism. However, conflicting reports on levels across different types limited research using human patient samples limit our understanding its diagnostic potential. This cross-sectional study explores concentrations gastric colon tissues CL-specific fluorescent probe MitoCLue compares them to adjacent healthy tissues. In cancer, showed no significant differences between tumor tissues, suggesting metabolic shifts do not affect total content. contrast, exhibited 33% increase levels, indicating adaptation and/or mass cancer. No associations were found demographic factors; although weak correlation with body index was noted. We successfully applied quantitatively assess from patients or The distinct suggest there cancer-type specific adaptations, reflecting unique bioenergetic demands reprogramming pathways. While observed compared this modest variation may utility as standalone biomarker.

Language: Английский

Multifunctional Polypeptide-Based Nanoconjugates for Targeted Mitochondrial Delivery and Nonviral Gene Therapy DOI Creative Commons
Camilla Pegoraro, Esther Masiá, Snežana Đorđević

et al.

Chemistry of Materials, Journal Year: 2025, Volume and Issue: 37(4), P. 1457 - 1467

Published: Feb. 5, 2025

Despite recent advances in nanomedicine, developing multifunctional nanocarriers capable of targeted subcellular delivery and efficient gene therapy remains a significant challenge. This study reports the design, synthesis, evaluation novel polypeptide-based nanoconjugate that addresses this gap using sequential delivery, combining mitochondrial targeting nonviral therapy. We engineered poly-l-ornithine-based, polyethylene glycol-modified carrier introduced custom-designed trivalent compound (TRV3) into structure. TRV3, conjugated to polypeptide via redox-sensitive disulfide linker, incorporates well-described triphenylphosphonium moiety (TPP) for Cy5 fluorophore as model drug. The resulting (C-TRV3-A) demonstrated endosomal escape localization. Leveraging endosomolytic properties C-TRV3-A, we explored its potential vector After optimizing formulation stability VLC-3 anionic coating, developed plasmid DNA polyplexes exhibited enhanced transfection efficiency basic advanced triple-negative breast cancer cell culture models. represents advance field, offering chemically versatile platform simultaneous may be used treatments, among other pathologies.

Language: Английский

Citations

0
A light-driven molecular motor-polypeptide conjugate supports controlled cell uptake DOI Creative Commons
Camilla Pegoraro, Ainoa Guinart, Esther Masiá

et al.

Journal of Materials Chemistry B, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

A light-driven molecular motor-polypeptide conjugate links a motor to mitochondria-targeted polyglutamate. Upon 405 nm light irradiation, it shifts from micelles worm-like structures, boosting cellular uptake and specificity.

Language: Английский

Citations

0
Total cardiolipin levels in gastric and colon cancer: evaluating the prognostic potential DOI Creative Commons
Pāvels Dimitrijevs,

Ilona Freiliba,

Andrejs Pčolkins

et al.

Lipids in Health and Disease, Journal Year: 2025, Volume and Issue: 24(1)

Published: Feb. 27, 2025

Cardiolipin (CL) is a signature phospholipid of mitochondria that maintains the integrity mitochondrial membrane and supports proper function. Alterations in CL level composition can impair or, conversely, improve function bioenergetics, both which are critical for cancer metabolism. However, conflicting reports on levels across different types limited research using human patient samples limit our understanding its diagnostic potential. This cross-sectional study explores concentrations gastric colon tissues CL-specific fluorescent probe MitoCLue compares them to adjacent healthy tissues. In cancer, showed no significant differences between tumor tissues, suggesting metabolic shifts do not affect total content. contrast, exhibited 33% increase levels, indicating adaptation and/or mass cancer. No associations were found demographic factors; although weak correlation with body index was noted. We successfully applied quantitatively assess from patients or The distinct suggest there cancer-type specific adaptations, reflecting unique bioenergetic demands reprogramming pathways. While observed compared this modest variation may utility as standalone biomarker.

Language: Английский

Citations

0