LysSYL‐Loaded pH‐Switchable Self‐Assembling Peptide Hydrogels Promote Methicillin‐Resistant Staphylococcus Aureus Elimination and Wound Healing DOI Creative Commons
He Liu, Xuemei Wei, Huagang Peng

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(52)

Published: Nov. 16, 2024

Abstract Staphylococcus aureus ( S. ), especially methicillin‐resistant (MRSA), causes wound infections, whose treatment remains a clinical challenge. Bacterium‐infected wounds often create acidic niches with pH 4.5–6.5. Endolysin LysSYL, which is derived from phage SYL, shows promise as an antistaphylococcal agent. However, endolysins generally exhibit instability and possess low bioavailability in microenvironments. Here, array of self‐assembling peptides designed, peptide L5 screened out based on its gel formation property bioavailability. exerted pH‐switchable antimicrobial effect (pH 5.5) formed biocompatible hydrogels at neutral 7.4). The LysSYL‐loaded can assemble L5@LysSYL hydrogels, increase thermal stability, the slow‐release LysSYL. Effective elimination achieved by through bacterial membrane disruption cell separation inhibition. Moreover, great potential promoting healing mouse model infected MRSA. Furthermore, are safe decrease cytokine levels number key factors for vessel formation, contribute to healing. Overall, effectively clean MRSA promote healing, suggests pH‐sensitive dressing management infections.

Language: Английский

Specifically targeted antimicrobial cyclic peptide to staphylococcal protein A for the treatment of S. aureus infection DOI

Xiaoling Lei,

Yong Li,

Xueting Cheng

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 162243 - 162243

Published: April 1, 2025

Language: Английский

Citations

0
Hydrogel-Based Bioactive Synthetic Skin Stimulates Regenerative Gas Signaling and Eliminates Interfacial Pathogens to Promote Burn Wound Healing DOI
Haifeng Zhang, Wei Zhou, Haibing Wang

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: April 10, 2025

Skin burn wounds (SBWs) are common clinical injuries due to excessive exposure factors including heat, radiation, chemical agents, etc. However, the efficient healing of SBWs is still challenging persistent inflammation and high risk local infection. To meet these challenges, we report a hydrogel-based bioactive synthetic skin (HBSS) from biocompatible components as dressing materials for wound treatment, which mediated localized H2S release stimulate tissue regeneration while preventing bacterial infection inflammation. Here, donor (N-(benzoyl mercapto) benzamide) was first coassembled with thioketal (TK)-ligated dopamine dimer form nanoscale assemblies (DDNs), were then integrated into Schiff base-cross-linked hyaluronic acid-carboxymethyl chitosan hydrogels. The elevated acidity in would trigger hydrogel degradation DDNs, further activated by ROS-induced cleavage TK linkers gas attenuating ROS stress self-immolative manner, thus promoting angiogenesis through activating AMPK RAS-MAPK-AP1 prohealing pathways, enabling M1-to-M2 macrophage reprogramming ERK1/2 NRF2 signaling. Meanwhile, network could inhibit colonization at site prevent These merits acted cooperative manner enable accelerated robust healing, offering an approach treatment clinic.

Language: Английский

Citations

0
Self-Adaptive Release of Stem Cell-Derived Exosomes from a Multifunctional Hydrogel for Accelerating MRSA-Infected Diabetic Wound Repair DOI
Xiang Wang,

Jiantong Dong,

Jingjing Kang

et al.

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: April 30, 2025

Chronic diabetic wounds are prone to severe skin necrosis and bacterial infections, with elevated reactive oxygen species (ROS) persistent inflammation further hindering the healing process. Developing smart dressings multifunctional therapeutic capabilities simultaneously combat reduce oxidative stress, alleviate inflammation, promote tissue regeneration remains a significant challenge. Here, we introduce self-adaptive yet hydrogel (Exo-Gel) designed accelerate methicillin-resistant Staphylococcus aureus (MRSA)-infected wound repair. Exo-Gel utilizes choline phosphate (CP) groups both anchor stem cell-derived exosomes (Exo) via electrostatic interactions disrupt membranes, providing inherent bacteriostatic effects. Additionally, ROS-responsive thioketal (TK) linkers enable release of based on local ROS levels while also scavenging excess ROS. This synergistic system facilitates by modulating reducing promoting M2 macrophage polarization, enhancing cell proliferation, myofibroblast migration, angiogenesis, collagen deposition regeneration. In Sprague-Dawley rats MRSA-infected full-thickness wounds, achieved remarkable activity accelerated healing. offers cost-effective, multifunctional, strategy for managing chronic requiring no external components or operations, making it highly practical translatable clinical applications.

Language: Английский

Citations

0
LysSYL‐Loaded pH‐Switchable Self‐Assembling Peptide Hydrogels Promote Methicillin‐Resistant Staphylococcus Aureus Elimination and Wound Healing DOI Creative Commons
He Liu, Xuemei Wei, Huagang Peng

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(52)

Published: Nov. 16, 2024

Abstract Staphylococcus aureus ( S. ), especially methicillin‐resistant (MRSA), causes wound infections, whose treatment remains a clinical challenge. Bacterium‐infected wounds often create acidic niches with pH 4.5–6.5. Endolysin LysSYL, which is derived from phage SYL, shows promise as an antistaphylococcal agent. However, endolysins generally exhibit instability and possess low bioavailability in microenvironments. Here, array of self‐assembling peptides designed, peptide L5 screened out based on its gel formation property bioavailability. exerted pH‐switchable antimicrobial effect (pH 5.5) formed biocompatible hydrogels at neutral 7.4). The LysSYL‐loaded can assemble L5@LysSYL hydrogels, increase thermal stability, the slow‐release LysSYL. Effective elimination achieved by through bacterial membrane disruption cell separation inhibition. Moreover, great potential promoting healing mouse model infected MRSA. Furthermore, are safe decrease cytokine levels number key factors for vessel formation, contribute to healing. Overall, effectively clean MRSA promote healing, suggests pH‐sensitive dressing management infections.

Language: Английский

Citations

3