Biosensors,
Journal Year:
2025,
Volume and Issue:
15(6), P. 362 - 362
Published: June 5, 2025
Colorimetric-based
biosensors
are
practical
detection
devices
that
can
detect
the
presence
and
concentration
of
biomarkers
through
simple
color
changes.
Conventional
laboratory-based
tests
highly
sensitive
but
require
long
processing
times
expensive
equipment,
which
makes
them
difficult
to
apply
for
on-site
diagnostics.
In
contrast,
colorimetric
method
offers
advantages
point-of-care
testing
real-time
monitoring
due
its
flexibility,
operation,
rapid
results,
versatility
across
many
applications.
order
enhance
change
reactions
in
techniques,
functional
nanomaterials
often
integrated
their
desirable
intrinsic
properties.
this
review,
working
principles
nanomaterial-based
strategies
systems
introduced.
addition,
current
signal
amplification
methods
comprehensively
outlined
evaluated.
Finally,
latest
trends
artificial
intelligence
(AI)
machine
learning
integration
into
colorimetric-based
biosensors,
including
potential
technological
advancements
near
future,
discussed.
Future
research
is
expected
develop
multifunctional
methods,
will
serve
as
powerful
alternatives
self-testing.
Microorganisms,
Journal Year:
2025,
Volume and Issue:
13(5), P. 1166 - 1166
Published: May 21, 2025
Urban
sewage,
aquaculture
wastewater,
and
medical
wastewater
are
significant
reservoirs
transmission
sources
of
Salmonella.
Rapid
detection
Salmonella
is
crucial
for
effectively
reducing
the
risk
disease
safeguarding
public
health.
Differentiating
viable
from
inactivated
cells
presents
challenges,
affecting
accurate
assessment
pathogen
risks.
Moreover,
current
methods
face
several
limitations,
including
lengthy
periods,
high
costs,
limited
applicability,
underscoring
need
rapid,
sensitive,
visual
diagnostic
approaches.
In
this
study,
we
combined
propidium
monoazide
(PMA)
with
recombinase
polymerase
amplification
(RPA)
clustered
regularly
spaced
short
palindromic
repeats
(CRISPR)/Cas12a
systems
to
develop
a
rapid
system
targeting
fimY
gene.
DNA
was
amplified
visually
detected
within
60
min
dead
were
excluded.
We
assessed
specificity
sensitivity
PMA-RPA-CRISPR/Cas12a
assay.
The
results
showed
that
assay
had
level
specificity,
no
reactions
observed
other
pathogens.
application
PMA
has
effect
on
RPA-CRISPR/Cas12a
technology
visibility
fluorescence
reporting
system.
successfully
in
minimum
limit
101
CFU/mL.
summary,
developed
study
allows
at
concentrations
as
low
By
integrating
technology,
offers
valuable
technical
support
efficient,
clear
wastewater.
Biosensors,
Journal Year:
2025,
Volume and Issue:
15(6), P. 362 - 362
Published: June 5, 2025
Colorimetric-based
biosensors
are
practical
detection
devices
that
can
detect
the
presence
and
concentration
of
biomarkers
through
simple
color
changes.
Conventional
laboratory-based
tests
highly
sensitive
but
require
long
processing
times
expensive
equipment,
which
makes
them
difficult
to
apply
for
on-site
diagnostics.
In
contrast,
colorimetric
method
offers
advantages
point-of-care
testing
real-time
monitoring
due
its
flexibility,
operation,
rapid
results,
versatility
across
many
applications.
order
enhance
change
reactions
in
techniques,
functional
nanomaterials
often
integrated
their
desirable
intrinsic
properties.
this
review,
working
principles
nanomaterial-based
strategies
systems
introduced.
addition,
current
signal
amplification
methods
comprehensively
outlined
evaluated.
Finally,
latest
trends
artificial
intelligence
(AI)
machine
learning
integration
into
colorimetric-based
biosensors,
including
potential
technological
advancements
near
future,
discussed.
Future
research
is
expected
develop
multifunctional
methods,
will
serve
as
powerful
alternatives
self-testing.
