Modulation of Aggregation of Covalent Organic Framework Nanosheets with Aggregation-Induced Emission Activity for Ultrasensitive No-Wash Fluorescent Immunosensor DOI

Haifei Wan,

Xingxiang Wang,

Meiling Ye

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: May 16, 2025

No-wash fluorescence immunosensors are favored because of their simple operation and precise results. However, cleverly designing two fluorescent materials so that interaction through antigen-antibody specific binding to produce significant changes poses a considerable challenge the design immunosensors. Herein, covalent organic framework nanosheets (CONs) with aggregation-induced emission (AIE) luminogens (AIEgens) were synthesized regulate aggregation AIEgens for an ultrasensitive no-wash immunosensor. The two-dimensional (2D) CONTFBE-PDAN exhibited excellent AIE activity. A Pt nanoparticle (PtNP)-modified AIE-CONTFBE-PDAN was designed as signal amplifier, in which PtNPs used bind antibody (Ab) via Pt-N/Pt-S bonds. After sandwich immunocomplexes formed by interaction, more compact stacking assembly maximizes restriction intramolecular rotation, vibration, motion, conformational planarization. This optimizes spatial alignment required efficient AIE, thereby yielding strong response. Using carcinoembryonic antigen 19-9 (CA 19-9) model tumor marker, sandwich-type immunosensor constructed. Since crystalline 2D contains lots AIEgens, sensitivity is expected improve greatly. showed good performance CA linear range 0.01 mU/mL 10 U/mL detection limit 3.3 μU/mL (S/N = 3), satisfactory real samples. To best our knowledge, this first time AIE-CONs have been immunosensing, provides new perspective trace markers.

Language: Английский

Modulation of Aggregation of Covalent Organic Framework Nanosheets with Aggregation-Induced Emission Activity for Ultrasensitive No-Wash Fluorescent Immunosensor DOI

Haifei Wan,

Xingxiang Wang,

Meiling Ye

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: May 16, 2025

No-wash fluorescence immunosensors are favored because of their simple operation and precise results. However, cleverly designing two fluorescent materials so that interaction through antigen-antibody specific binding to produce significant changes poses a considerable challenge the design immunosensors. Herein, covalent organic framework nanosheets (CONs) with aggregation-induced emission (AIE) luminogens (AIEgens) were synthesized regulate aggregation AIEgens for an ultrasensitive no-wash immunosensor. The two-dimensional (2D) CONTFBE-PDAN exhibited excellent AIE activity. A Pt nanoparticle (PtNP)-modified AIE-CONTFBE-PDAN was designed as signal amplifier, in which PtNPs used bind antibody (Ab) via Pt-N/Pt-S bonds. After sandwich immunocomplexes formed by interaction, more compact stacking assembly maximizes restriction intramolecular rotation, vibration, motion, conformational planarization. This optimizes spatial alignment required efficient AIE, thereby yielding strong response. Using carcinoembryonic antigen 19-9 (CA 19-9) model tumor marker, sandwich-type immunosensor constructed. Since crystalline 2D contains lots AIEgens, sensitivity is expected improve greatly. showed good performance CA linear range 0.01 mU/mL 10 U/mL detection limit 3.3 μU/mL (S/N = 3), satisfactory real samples. To best our knowledge, this first time AIE-CONs have been immunosensing, provides new perspective trace markers.

Language: Английский

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