Electroconductive Bionanocomposites from Black Soldier Fly Proteins for Green Flexible Electronics DOI Creative Commons

Edoardo Testa,

Vincenzina Barbera, Elisa Fasoli

et al.

ACS Sustainable Chemistry & Engineering, Journal Year: 2025, Volume and Issue: 13(6), P. 2388 - 2400

Published: Feb. 3, 2025

Printed and flexible electronics hold the potential to revolutionize world of electronic devices. A primary focus today is their circularity, which can be achieved by using biobased materials. In this study, electrically conductive bionanocomposite materials suitable for were fabricated proteins from black soldier fly (BSF, Hermetia illucens). The valorization BSF biomacromolecules currently being pursued in framework emerging circular economy models bioconversion Organic Fraction Municipal Solid Waste (OFMSW), where has been demonstrated act as an extremely efficient bioconverter provide lipids, chitin, proteins. Here, protein extracts characterized proteomic techniques, revealing a pool myofibrillar able interact through intermolecular β-sheet interactions. Flexible electroconductive next formulated combining with carbon (CCB), either its pristine form or functionalized 2-(2,5-dimethyl-1H-pyrrol-1-yl)-1,3-propanediol (serinol pyrrole, SP), water only solvent incorporating glycerol carboxymethylcellulose (CMC) additional green ingredients. sustainable, low-pressure cold plasma (LPCP) technology was ultimately proposed achieve high film surface hydrophobicity. Characterized effective biodegradability, strain-sensing properties, electrical conductivity (up 0.9 × 10-2 S/cm at filler content 8% v/v (15% w/w)), hydrophobicity, bionanocomposites presented here may well suited disposable electronics, wearable devices, electrostatic discharge fabrics, packaging, hence offering new routes toward OFMSW development electronics.

Language: Английский

Food amyloid fibrils are safe nutrition ingredients based on in-vitro and in-vivo assessment DOI Creative Commons
Dan Xu, Jiangtao Zhou, Wei Long Soon

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Oct. 26, 2023

Food protein amyloid fibrils have superior technological, nutritional, sensorial, and physical properties compared to native monomers, but there is as yet insufficient understanding of their digestive fate safety for wide consumption. By combining SDS-PAGE, ELISA, fluorescence, AFM, MALDI-MS, CD, microfluidics, SAXS techniques the characterization β-lactoglobulin lysozyme subjected in-vitro gastrointestinal digestion, here we show that either no noticeable conformational differences exist between aggregates monomer counterparts after digestion process (as in β-lactoglobulin), or are digested significantly better than monomers lysozyme). Moreover, exposure human cell lines in-vivo studies with C. elegans mouse models, indicate present observable cytotoxicity, physiological abnormalities health-span, nor accumulation fibril-induced plaques brain other organs. These extensive together suggest food amyloids at least equally safe those obtained from corresponding pointing potential ingredients nutrition.

Language: Английский

Citations

55
Production and characterization of amaranth amyloid fibrils from food protein waste DOI Creative Commons
Svitlana Mykolenko, Wei Long Soon, Raffaele Mezzenga

et al.

Food Hydrocolloids, Journal Year: 2023, Volume and Issue: 149, P. 109604 - 109604

Published: Dec. 2, 2023

Plant proteins self-assembly into amyloid fibrils is a promising modification to be introduced in emerging food and materials applications. Although the valorization of protein-rich side streams amaranth oil production completely missing, we show that double salt extraction provides recovery globulin fraction seed from waste with high purity complete protein solubility at acidic pH. We demonstrate silico propensity density amyloidogenic regions 11S storage Amaranthus hypochondriacus. further recovered can self-assemble under heat-induced hydrolysis. The nature was studied by thiazole orange (TO) fluorescence, SDS-PAGE, FTIR, CD, TEM, AFM. systematically investigated how temperature fibrillization affected unfolding, peptide release, as well its effect on tuning formation, growth, polymorphism fibrils. β-sheet-rich structured extracted exhibited mesoscopic rigid manifestation left-handed ribbons varying polymorphism. favourable conditions for extensive hydrolysis promote growth well-ordered thick twisted small-pitch extraordinary rigidity interpret these finding based structural models These results provide additional insight formation plant functional foods biocompatible materials, introduce pathway convert nanofibrils.

Language: Английский

Citations

24
From Soy Waste to Bioplastics: Industrial Proof of Concept DOI
Massimo Bagnani, Mohammad Peydayesh,

Thomas Knapp

et al.

Biomacromolecules, Journal Year: 2024, Volume and Issue: 25(3), P. 2033 - 2040

Published: Feb. 8, 2024

The global plastic waste problem is pushing for the development of sustainable alternatives, encouraged by stringent regulations combined with increased environmental consciousness. In response, this study presents an industrial-scale proof concept to produce self-standing, transparent, and flexible bioplastic films, offering a possible solution pollution resource valorization. We achieve combining amyloid fibrils self-assembled from food methylcellulose glycerol. Specifically, soy whey okara, two pivotal protein-rich byproducts tofu manufacturing, emerge as versatile precursors fibril formation development. An exhaustive feasibility involving transformation 500 L into ∼1 km (27 kg) films underscores potential technology. To extend practicality our approach, we further processed running kilometer film at industrial scale transparent windows paper-based packaging. mechanical properties water interactions novel are tested compared those commercially used films. By pioneering large-scale production biodegradable bioplastics sourced byproducts, work not only simultaneously addresses dual challenges but also practically demonstrates biopolymeric building block valorization materials in real-world scenarios.

Language: Английский

Citations

15
Fibrillisation of faba bean protein isolate by thermosonication for process efficacy: Microstructural characteristics, assembly behaviour, and physicochemical properties DOI Creative Commons
Y. Hu, Lirong Cheng, Elliot P. Gilbert

et al.

Food Hydrocolloids, Journal Year: 2024, Volume and Issue: 154, P. 110127 - 110127

Published: April 22, 2024

The effect of thermosonication (TS) (90 °C, 10-30 min) on the fibrillisation faba bean protein isolate (FPI) was studied. self-assembly behaviour, microstructural characteristics and techno-functional (gelation emulsification) properties FPI fibrils obtained from TS treatment were compared with those conventional prolonged heating (CH) at 90 °C up to 8 h. Compared CH treatment, shown significantly accelerate formation prominent β-sheet structures as revealed by Thioflavin T (ThT) fluorescence, Fourier-transform infrared spectroscopy (FTIR) circular dichroism (CD). fibril building blocks analysed sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) liquid chromatography linked tandem mass spectrometry (LC-MS/MS) obtain differences between induced FPI. Transmission electron microscopy (TEM) small-angle neutron scattering (SANS) showed that 4 h 10 min treatments resulted in similar radius (from 5-10 nm). Furthermore, SANS indicated an entangled fibrillar network, which could lead observed viscoelastic a high concentration (10 wt%). Finally, internal phase O/W emulsions (HIPE, ϕ=0.75) stabilised 30 (3 wt%) demonstrated stronger strength smaller oil droplet size prepared untreated FPI, suggesting superior emulsification capability fibrils. This finding demonstrates is promising efficient method for plant proteins resultant generating excellent gelation properties.

Language: Английский

Citations

11
Using silver nanoparticle-decorated whey protein isolate amyloid fibrils to modify the electrode surface used for electrochemical detection of para-nitrophenol DOI
You-Ren Lai,

Steven S-S Wang,

Ta‐Hsien Lin

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 264, P. 130404 - 130404

Published: Feb. 27, 2024

Language: Английский

Citations

9
High Captured Carbon Content Bioplastic Film from Spirulina DOI Open Access
Jia Bin Yeo, Jun Ho Jang,

Jeong Woo Koo

et al.

Advanced Sustainable Systems, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 5, 2025

Abstract Bioplastics offer a solution to the problem of increasing atmospheric CO 2 levels contributed by linear production and consumption pattern fossil‐derived plastics closing carbon loop with material sourcing from biomass bioplastic's biodegradability. Spirulina, genus cyanobacteria that is capable rapid uptake, can be key realizing circular plastic economy converting value‐added high protein content biomass, which extracted processed produce protein‐based bioplastics captured biodegradable, releasing after decomposition returned cycle. To demonstrate feasibility utilizing self‐assembling capabilities Spirulina fabricate bioplastics, simple procedure proposed involves isoelectric point precipitation followed solvent casting an entirely bioplastic film containing glycerol as plasticizer without blending other types conventional plastics. By further crosslinking carboxymethylcellulose Ca 2+ cations, are able obtain films highest average tensile strength 5.5MPa. Additionally, showed potential in UV‐blocking applications exhibited good

Language: Английский

Citations

1
Sustainable amyloid fibrils production from tofu wastewater DOI Creative Commons
Svitlana Mykolenko, Raffaele Mezzenga

Food Hydrocolloids, Journal Year: 2025, Volume and Issue: unknown, P. 111072 - 111072

Published: Jan. 1, 2025

Language: Английский

Citations

1
Exploring the interfacial behavior and foam characteristics of various soy protein aggregates: Insights of morphology and conformational flexibility DOI
Ri‐Qi Su, Xueqing Zhang,

Jia Jia Cao

et al.

Food Hydrocolloids, Journal Year: 2025, Volume and Issue: unknown, P. 111362 - 111362

Published: March 1, 2025

Language: Английский

Citations

1
Formation and structural characteristics of pea globulin amyloid-like fibrils pretreated with low-frequency magnetic field DOI
Qingqing Liu, Yang Qin, Yaru Wang

et al.

Food Hydrocolloids, Journal Year: 2023, Volume and Issue: 147, P. 109331 - 109331

Published: Sept. 23, 2023

Language: Английский

Citations

21
From Fundamental Amyloid Protein Self-Assembly to Development of Bioplastics DOI Creative Commons
Tianchen Li,

Jordan Kambanis,

Timothy L. Sorenson

et al.

Biomacromolecules, Journal Year: 2023, Volume and Issue: 25(1), P. 5 - 23

Published: Dec. 26, 2023

Proteins can self-assemble into a range of nanostructures as result molecular interactions. Amyloid nanofibrils, one them, were first discovered with regard to the relevance neurodegenerative diseases but now have been exploited building blocks generate multiscale materials designed functions for versatile applications. This review interconnects mechanism amyloid fibrillation, current approaches synthesizing protein-based materials, and application in bioplastic development. We focus on fundamental structures self-assembled fibrils how external factors affect protein aggregation optimize process. Protein self-assembly is essentially autonomous congregation smaller units larger, organized structures. Since properties be manipulated by changing intrinsic conditions, serves an excellent block Building these principles, general processing methods pathways from raw sources mature state are proposed, providing guide development large-scale production. Additionally, this discusses diverse nanofibrils they utilized bioplastics. The economic feasibility bioplastics also compared conventional plastics production scenarios, supporting their potential sustainable future

Language: Английский

Citations

21