Engineered Ratiometric Sensory Electrospun Fibers for Oxygen Mapping in Complex Cultures and Tumor Microenvironment DOI Creative Commons
Giuliana Grasso, Stefania Forciniti, Valentina Onesto

et al.

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 283, P. 117481 - 117481

Published: April 17, 2025

Monitoring the hypoxic microenvironment is fundamental due to its implication in tumor aggressiveness and progression. In this work, we propose fabrication of ratiometric fluorescent fibers via electrospinning poly(trimethylsylil)propine (PTMSP) polymer, an optically clear gas permeable for oxygen (O2) sensing a melanoma model. The configuration was obtained by entrapping tris(4,7-diphenyl-1,10-phenanthroline) ruthenium (II) dichloride, capable detecting dissolved O2 variations, together with rhodamine B isothiocyanate, serving as reference dye, within polymer matrix. were characterized point out morphology, porosity, hydrophilicity. ability fibrous mat deeply investigated means microplate reader confocal imaging, showing strict correlation between read-out increasing concentration aqueous-based media. Moreover, exhibited high photostability, reversibility excellent cytocompatibility, allowing monitoring gradients over time space vitro co-cultures. Overall, optimized micrometric system holds potential real-time assessments levels complex cell systems heterogeneous tumour microenvironments, can open up new engineering possibilities using O2-sensitive dyes tissue scaffolding strategies.

Language: Английский

Advancing Biosensing through Super-Resolution Fluorescence Microscopy DOI

Ga‐eun Go,

Doory Kim

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: unknown, P. 117374 - 117374

Published: March 1, 2025

Language: Английский

Citations

0
Engineered Ratiometric Sensory Electrospun Fibers for Oxygen Mapping in Complex Cultures and Tumor Microenvironment DOI Creative Commons
Giuliana Grasso, Stefania Forciniti, Valentina Onesto

et al.

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 283, P. 117481 - 117481

Published: April 17, 2025

Monitoring the hypoxic microenvironment is fundamental due to its implication in tumor aggressiveness and progression. In this work, we propose fabrication of ratiometric fluorescent fibers via electrospinning poly(trimethylsylil)propine (PTMSP) polymer, an optically clear gas permeable for oxygen (O2) sensing a melanoma model. The configuration was obtained by entrapping tris(4,7-diphenyl-1,10-phenanthroline) ruthenium (II) dichloride, capable detecting dissolved O2 variations, together with rhodamine B isothiocyanate, serving as reference dye, within polymer matrix. were characterized point out morphology, porosity, hydrophilicity. ability fibrous mat deeply investigated means microplate reader confocal imaging, showing strict correlation between read-out increasing concentration aqueous-based media. Moreover, exhibited high photostability, reversibility excellent cytocompatibility, allowing monitoring gradients over time space vitro co-cultures. Overall, optimized micrometric system holds potential real-time assessments levels complex cell systems heterogeneous tumour microenvironments, can open up new engineering possibilities using O2-sensitive dyes tissue scaffolding strategies.

Language: Английский

Citations

0