Scaffolding Biomaterials for 3D Cultivated Meat: Prospects and Challenges

Advanced Science, Journal Year: 2021, Volume and Issue: 9(3)

Published: Nov. 16, 2021

Abstract Cultivating meat from stem cells rather than by raising animals is a promising solution to concerns about the negative externalities of production. For cultivated fully mimic conventional meat's organoleptic and nutritional properties, innovations in scaffolding technology are required. Many technologies already developed for use biomedical tissue engineering. However, production comes with unique set constraints related scale cost as well necessary attributes final product, such texture food safety. This review discusses properties vertebrate skeletal muscle that will need be replicated successful product current state innovation within industry, highlighting scaffold materials techniques can applied development. Recommendations provided future research into scaffolds capable supporting growth high‐quality while minimizing costs. Although development appropriate challenging, it also tractable provides novel opportunities customize properties.

Language: Английский

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Ex-ante life cycle assessment of commercial-scale cultivated meat production in 2030

The International Journal of Life Cycle Assessment, Journal Year: 2023, Volume and Issue: 28(3), P. 234 - 254

Published: Jan. 12, 2023

Abstract Purpose Cultivated meat (CM) is attracting increased attention as an environmentally sustainable and animal-friendly alternative to conventional meat. As the technology matures, more data are becoming available uncertainties decline. The goal of this ex-ante life cycle assessment (LCA) was provide outlook environmental performance commercial-scale CM production in 2030 compare animal 2030, using recent often primary data, combined with scenario analysis. Methods This comparative attributional LCA used ReCiPe Midpoint impact method. System boundaries were cradle-to-gate, functional unit 1 kg Data collected from over 15 companies active its supply chain. Source include lab-scale five producers, full-scale processes comparable manufacturing fields, computational models, published literature. Important have been cross-checked additional experts. Scenarios represent variation assess influence important choices such energy mix. Ambitious benchmarks made for beef, pork, chicken systems, which efficient intensive European agriculture incorporate potential improvements 2030. Results discussion almost three times turning crops into than chicken, most animal, therefore agricultural land use low. Nitrogen-related air pollution emissions also lower because efficiency produced a contained system without manure. energy-intensive, mix chain important. Using renewable energy, carbon footprint beef pork ambitious benchmark chicken. Greenhouse gas profiles different, being mostly CO 2 CH 4 N O meats. Climate hotspots maintaining temperature reactors biotechnological culture medium ingredients. Conclusions has benchmarks, indicators, clearly use, pollution, nitrogen-related emissions. substantially that beef. How it compares depends on mixes. While upstream can ensure all Recommendations producers should optimize source leverage collaborations feedstocks, search optimum through combining low-impact ingredients high-performance formulation. Governments consider emerging industry’s demand sustainability freed-up land. Consumers not extra option menu, but substitute higher-impact products.

Language: Английский

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Strategies to improve meat-like properties of meat analogs meeting consumers’ expectations

Biomaterials, Journal Year: 2022, Volume and Issue: 287, P. 121648 - 121648

Published: June 25, 2022

Language: Английский

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Tissue-like cultured fish fillets through a synthetic food pipeline

npj Science of Food, Journal Year: 2023, Volume and Issue: 7(1)

Published: May 6, 2023

Tissue-like cultured meats of some livestock have successfully been established by different approaches. However, the production a structure similar to fish fillets is still challenging. Here, we develop tissue-like assembly large yellow croaker muscle fibers and adipocytes with 3D-printed gel. Inhibition Tgf-β Notch signals significantly promoted myogenic differentiation piscine satellite cells (PSCs). The mixture gelatin sodium alginate combined p53 inhibitor Yap activator supported PSC viability proliferation. Based on texture tissue, 3D scaffold was constructed gelatin-based gel mixed PSCs. After proliferation differentiation, filled adipocytes. Finally, 20 × 12 4 mm were formed, consisting 5.67 107 muscles 4.02 biomanufacture fillet here could be promising technology customize meat high fidelity.

Language: Английский

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Soy Protein Amyloid Fibril Scaffold for Cultivated Meat Application

ACS Applied Materials & Interfaces, Journal Year: 2023, Volume and Issue: 15(12), P. 15108 - 15119

Published: March 14, 2023

It is important to have sustainable and edible scaffolds produce cultivated meat. In this research, three-dimensional (3D) porous were developed by soy protein amyloid fibrils for meat applications. Food-safe biological physical cross-linking methods using microbial transglutaminase temperature-controlled water vapor annealing technique employed crosslink fibrils, resulting in the production of 3D scaffolds. The generated had pores with sizes ranging from 50 250 μm, porosities 72-83%, compressive moduli 3.8-4.2 kPa, depending on type used process (β-conglycinin (7S), glycinin (11S) isolate (SPI)). When present pepsin, these can degrade within an hour but remain stable phosphate-buffered saline at least 30 days. fibril enabled C2C12 mouse skeletal myoblasts proliferate differentiate without adding cell adhesive proteins or other coatings. results demonstrate potential abundant inexpensive be utilized as scaffold materials food industry.

Language: Английский

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Tissue Engineering Challenges for Cultivated Meat to Meet the Real Demand of a Global Market

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(7), P. 6033 - 6033

Published: March 23, 2023

Cultivated meat (CM) technology has the potential to disrupt food industry-indeed, it is already an inevitable reality. This new alternative solve environmental, health and ethical issues associated with demand for products. The global market longs biotechnological improvements CM production chain. CM, also known as cultured, cell-based, lab-grown, in vitro or clean meat, obtained through cellular agriculture, which based on applying tissue engineering principles. In practice, first necessary choose best cell source type, then furnish nutrients, growth factors signalling molecules via cultivation media. procedure occurs a controlled environment that provides surfaces anchor-dependent cells offers microcarriers scaffolds favour three-dimensional (3D) organisation of multiple types. this review, we discuss relevant information production, including process, sources, medium requirements, main obstacles (consumer acceptance, scalability, safety reproducibility), technological aspects 3D models (biomaterials, scaffolds) assembly methods (cell layering, spinning bioprinting). We provide outlook market. Our review brings broad overview field, providing update everyone interested topic, especially important because multidisciplinary technology.

Language: Английский

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Antimicrobial Activities of Natural Bioactive Polyphenols

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(6), P. 718 - 718

Published: May 27, 2024

Secondary metabolites, polyphenols, are widespread in the entire kingdom of plants. They contain one or more hydroxyl groups that have a variety biological functions natural environment. These uses include polyphenols food, beauty products, dietary supplements, and medicinal products grown rapidly during past 20 years. Antimicrobial described together with their sources, classes, subclasses. Polyphenols found different such as dark chocolate, olive oil, red wine, almonds, cashews, walnuts, berries, green tea, apples, artichokes, mushrooms, etc. Examples benefits antiallergic, antioxidant, anticancer agents, anti-inflammatory, antihypertensive, antimicrobe properties. From these classes helpful for growth internal functional systems human body, providing healthy fats, vitamins, minerals, lowering risk cardiovascular diseases, improving brain health, rebooting our cellular microbiome health by mitochondrial uncoupling. Among various (curcumin, naringenin, quercetin, catechin, etc.) primarily antimicrobial activities discussed along possible future applications. For agents to be proven safe, adverse impacts must substantiated reliable scientific research well vitro vivo clinical data. Future may influenced this evaluation.

Language: Английский

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Advancements in plant based meat analogs enhancing sensory and nutritional attributes

npj Science of Food, Journal Year: 2024, Volume and Issue: 8(1)

Published: Aug. 7, 2024

The burgeoning demand for plant-based meat analogs (PBMAs) stems from environmental, health, and ethical concerns, yet replicating the sensory attributes of animal remains challenging. This comprehensive review explores recent innovations in PBMA ingredients methodologies, emphasizing advancements texture, flavor, nutritional profiles. It chronicles transition soy-based first-generation products to more diversified second- third-generation PBMAs, showcasing utilization various plant proteins advanced processing techniques enrich experiences. underscores crucial role proteins, polysaccharides, fats mimicking meat's texture flavor emphasizes research on new sources improve product quality. Addressing challenges like production costs, taste, adequacy is vital enhancing consumer acceptance fostering a sustainable food system.

Language: Английский

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Growing meat on autoclaved vegetables with biomimetic stiffness and micro-patterns

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 2, 2025

Cultured meat needs edible bio-scaffolds that provide not only a growth milieu for muscle and adipose cells, but also biomimetic stiffness tissue-sculpting topography. Current meat-engineering technologies struggle to achieve scalable cell production, efficient differentiation, tissue maturation in one single culture system. Here we propose an autoclaving strategy transform common vegetables into muscle- adipose-engineering scaffolds, without undergoing conventional plant decellularization. We selected with natural anisotropic isotropic topology mimicking microstructures respectively. further adjusted vegetable by autoclaving, emulate the mechanical properties of animal tissues. Autoclaved preserve rich cell-affinitive moieties, yielding good effect simplified processing. Chinese chive Shiitake mushroom micro-patterns support expansion improved alignment myogenesis. loofah encourages adipocyte proliferation lipid accumulation. Our engineered fat-on-vegetables can construct stuffing or layered chips. possess tissue-mimicking topology, bring biochemical benefits, operational ease, cost reduction bioreactor compatibility. Without needing decellularization, these biomaterials may see scale-up applications analog bio-fabrication. Traditional cultured scaffolds employ animal-derived intricate manufacturing, plant-based offer appealing alternative. authors present simple shortcut method bioscaffolds via which mimics microstructure.

Language: Английский

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Scalable production of muscle and adipose cell-laden microtissues using edible macroporous microcarriers for 3D printing of cultured fish fillets

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 18, 2025

Cellular agriculture is a novel platform for addressing the issues of protein scarcity, environmental pressures, and food safety. However, expanding seed cells at large scale remains prerequisite achieving industrial cultured meat production. We here propose production large-pore-sized edible porous microcarriers (EPMs) by using NaCl to precisely control ice crystal growth during cryogenic crosslinking. Muscle satellite (SCs) adipose-derived stem (ASCs) from yellow croakers exhibit remarkable adhesion, proliferation, differentiation on gelatin-based EPMs. Following consecutive expansion, SCs ASCs densities reach 6.25 × 105 5.77 cells/mL, leading 499-fold 461-fold increase in cell numbers, respectively. Subsequently, mature microtissues are incorporated into bioink, thereby enabling successful three-dimensional printing fish fillets with sensory properties similar native fillets. EPM-based expansion structuring techniques promising facilitating large-scale Cell big challenge growing field cellular agriculture. Here, authors present an approach muscle adipose cell-laden 3D

Language: Английский

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