Nano Research, Journal Year: 2022, Volume and Issue: 16(1), P. 905 - 916
Published: Aug. 17, 2022
Language: Английский
Bioactive Materials, Journal Year: 2023, Volume and Issue: 26, P. 194 - 215
Published: March 4, 2023
Over the years, electroconductive hydrogels (ECHs) have been extensively applied for stimulating nerve regeneration and restoring locomotor function after peripheral injury (PNI) with diabetes, given their favorable mechanical electrical properties identical to endogenous tissue. Nevertheless, PNI causes loss of inflammatory pain, especially in diabetic patients. It has established that bone marrow stem cells-derived exosomes (BMSCs-Exos) analgesic, anti-inflammatory tissue properties. Herein, we designed an ECH loaded BMSCs-Exos (ECH-Exos) dressing treat achieve functional recovery pain relief. Given its potent adhesive self-healing properties, this laminar is convenient treatment damaged fibers by automatically wrapping around them form a size-matched tube-like structure, avoiding cumbersome implantation process. Our vitro studies showed ECH-Exos could facilitate attachment migration Schwann cells. Meanwhile, Exos system modulate M2 macrophage polarization via NF-κB pathway, thereby attenuating PNI. Additionally, enhanced myelinated axonal MEK/ERK pathway vivo, consequently ameliorating muscle denervation atrophy further promoting restoration. findings suggest huge prospects regeneration, restoration relief patients
Language: Английский
Citations
51
CNS Neuroscience & Therapeutics, Journal Year: 2023, Volume and Issue: 29(4), P. 1094 - 1108
Published: Jan. 10, 2023
Abstract Background A growing body of research shows that drug monomers from traditional Chinese herbal medicines have antineuroinflammatory and neuroprotective effects can significantly improve the recovery motor function after spinal cord injury (SCI). Here, we explore role molecular mechanisms Alpinetin on activating microglia‐mediated neuroinflammation neuronal apoptosis SCI. Methods Stimulation microglia with lipopolysaccharide (LPS) to simulate models in vitro, effect release pro‐inflammatory mediators LPS‐induced its mechanism were detected. In addition, a co‐culture system cells was constructed assess apoptosis. Finally, rat used study inflammation, apoptosis, axonal regeneration, Alpinetin. Results inhibits activity JAK2/STAT3 pathway. also reverse activated reactive oxygen species (ROS) production decrease mitochondrial membrane potential (MMP) PC12 cells. vivo inflammatory response improves function. Conclusion be treat neurodegenerative diseases is novel candidate for treatment neuroinflammation.
Language: Английский
Citations
48
Journal of Controlled Release, Journal Year: 2023, Volume and Issue: 356, P. 463 - 480
Published: March 15, 2023
Complexity and difficulties in wound management are pressing concerns that affect patients' quality of life may result tissue infection, necrosis, loss local systemic functions. Hence, novel approaches to accelerate healing being actively explored over the last decade. Exosomes as important mediators intercellular communications promising natural nanocarriers due their biocompatibility, low immunogenicity, drug loading targeting capacities, innate stability. More importantly, exosomes developed a versatile pharmaceutical engineering platform for repair. This review provides an overview biological physiological functions derived from variety origins during phases, strategies exosomal engineering, therapeutic applications skin regeneration.
Language: Английский
Citations
48
ACS Nano, Journal Year: 2023, Volume and Issue: 17(18), P. 18562 - 18575
Published: Sept. 14, 2023
The treatment of spinal cord injury (SCI) remains unsatisfactory owing to the complex pathophysiological microenvironments at site and limited regenerative potential central nervous system. Metformin has been proven in clinical animal experiments repair damaged structures functions by promoting endogenous neurogenesis. However, early stage acute SCI, adverse microenvironment sites, such as reactive oxygen species inflammatory factor storm, can prevent activation neural stem cells (NSCs) differentiation NSCs into neurons, decreasing whole effect. To address those issues, a series robust multifunctional natural polyphenol-metformin nanoparticles (polyphenol-Met NPs) were fabricated with pH-responsiveness excellent antioxidative capacities. resulting NPs possessed several favorable advantages: First, composed active ingredients different biological properties, without need for carriers; second, feature could allow targeted drug delivery injured site; more importantly, enabled drugs performances exhibit strong synergistic effects. results demonstrated that improved polyphenols boosted activated neurons oligodendrocytes, which efficiently nerve enhance functional recovery SCI rats. This work highlighted design fabrication via efficient microenvironmental regulation activation.
Language: Английский
Citations
43
Advanced Science, Journal Year: 2024, Volume and Issue: 11(13)
Published: Jan. 29, 2024
Abstract Delayed wound healing is a major complication of diabetes, and associated with impaired cellular functions. Current treatments are unsatisfactory. Based on the previous reports microRNA expression in small extracellular vesicles (sEVs), miR‐17‐5p‐engineered sEVs (sEVs 17‐OE ) encapsulated them gelatin methacryloyl (GelMA) hydrogel for diabetic wounds treatment fabricated. SEVs successfully fabricated 16‐fold increase miR‐17‐5p expression. inhibited senescence promoted proliferation, migration, tube formation high glucose‐induced human umbilical vein endothelial cells (HG‐HUVECs). Additionally, also performs promotive effect dermal fibroblasts (HG‐HDFs). Mechanism analysis showed expressions p21 phosphatase tensin homolog (PTEN), as target genes miR‐17‐5p, downregulated significantly by . Accordingly, downstream pathways PTEN, activated. Next, loaded GelMA to fabricate novel bioactive dressing evaluate their effects healing. Gel‐sEVs effectively accelerated promoting angiogenesis collagen deposition. The mechanism may be local cell proliferation. Therefore, loading hydrogel, offering an option chronic management
Language: Английский
Citations
29
Bioactive Materials, Journal Year: 2024, Volume and Issue: 38, P. 1 - 30
Published: April 23, 2024
Characterized by their pivotal roles in cell-to-cell communication, cell proliferation, and immune regulation during tissue repair, exosomes have emerged as a promising avenue for "cell-free therapy" clinical applications. Hydrogels, possessing commendable biocompatibility, degradability, adjustability, physical properties akin to biological tissues, also found extensive utility engineering regenerative repair. The synergistic combination of hydrogels holds the potential not only enhance efficiency but collaboratively advance repair process. This review has summarized advancements made over past decade research hydrogel-exosome systems regenerating various tissues including skin, bone, cartilage, nerves tendons, with focus on methods encapsulating releasing within hydrogels. It critically examined gaps limitations current research, whilst proposed future directions applications this innovative approach.
Language: Английский
Citations
22
Theranostics, Journal Year: 2024, Volume and Issue: 14(5), P. 2099 - 2126
Published: Jan. 1, 2024
Exosomes, which are small vesicles enclosed by a lipid bilayer and released many cell types, widely dispersed have garnered increased attention in the field of regenerative medicine due to their ability serve as indicators diseases agents with therapeutic potential. Exosomes play crucial role mediating intercellular communication through transfer biomolecules, including proteins, lipids, RNA, other molecular constituents, between cells. The targeted transport proteins nucleic acids specific cells has potential enhance or impair biological functions. applications, they can be used alone combination approaches. examination unique attributes functions these factors emerged prominent study realm biomedical research. This manuscript summarizes origins properties exosomes, structural, biological, physical, chemical aspects. paper offers complete recent progress tissue repair medicine, emphasizing possible implications methods forthcoming regeneration attempts.
Language: Английский
Citations
20
Bioactive Materials, Journal Year: 2024, Volume and Issue: 34, P. 381 - 400
Published: Jan. 6, 2024
Preventing local tumor recurrence while promoting bone tissue regeneration is an urgent need for osteosarcoma treatment. However, the therapeutic efficacy of traditional photosensitizers limited, and they lack ability to regenerate bone. Here, a piezo-photo nanoheterostructure developed based on ultrasmall bismuth/strontium titanate nanocubes (denoted as Bi/SrTiO3), which achieve piezoelectric field-driven fast charge separation coupling with surface plasmon resonance efficiently generate reactive oxygen species. These hybrid nanotherapeutics are integrated into injectable biopolymer hydrogels, exhibit outstanding anticancer effects under combined irradiation NIR ultrasound. In vivo studies using patient-derived xenograft models tibial demonstrate that hydrogels suppression rates 98.6 % 67.6 in respective models. Furthermore, hydrogel had good filling retention capabilities defect region, exerted repair by polarizing conveying electrical stimuli cells mild ultrasound radiation. This study provides comprehensive clinically feasible strategy overall treatment osteosarcoma.
Language: Английский
Citations
19
Advanced Materials, Journal Year: 2024, Volume and Issue: 36(19)
Published: Feb. 8, 2024
Cavernous nerve injury (CNI), resulting in erectile dysfunction (ED), poses a significant threat to the quality of life for men. Strategies utilizing conductive hydrogels have demonstrated promising results treatment peripheral nerves with large diameter (>2 mm). However, integrating convenient minimally invasive operation, antiswelling and immunomodulatory treating small-diameter injured cavernous remains great challenge. Here, sprayable adhesive hydrogel (GACM) composed gelatin, adenine, carbon nanotubes, mesaconate designed repair is developed. Multiple hydrogen bonds provide GACM excellent properties, enabling it establish conformal electrical bridge damaged aiding regeneration process. Additionally, mesaconate-loaded suppresses release inflammatory factors by macrophages promotes migration proliferation Schwann cells. In vivo tests demonstrate that repairs restores function fertility. Furthermore, feasibility robotic surgery beagles validated. Given benefits therapeutic effectiveness clinical convenience, research suggests future materials as advanced solutions repair.
Language: Английский
Citations
19
Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)
Published: Jan. 3, 2024
Abstract Electroconductive hydrogels offer a promising avenue for enhancing the repair efficacy of spinal cord injuries (SCI) by restoring disrupted electrical signals along cord’s conduction pathway. Nonetheless, application composed diverse electroconductive materials has demonstrated limited capacity to mitigate post-SCI inflammatory response. Recent research indicated that transplantation M2 microglia effectively fosters SCI recovery attenuating excessive Exosomes (Exos), small vesicles discharged cells carrying similar biological functions their originating cells, present compelling alternative cellular transplantation. This investigation endeavors exploit microglia-derived exosomes (M2-Exos) successfully isolated and reversibly bonded through hydrogen bonding synergistic promotion synergistically enhance repair. In vitro experiments substantiated significant M2-Exos-laden stimulate growth neural stem axons in dorsal root ganglion modulate microglial polarization. Furthermore, M2-Exos remarkable ability initial reaction within injury site. When combined with hydrogel, worked expedite neuronal axonal regeneration, substantially functional rats afflicted SCI. These findings underscore potential as valuable reparative factor, amplifying foster rehabilitation.
Language: Английский
Citations
18