Tumor‐derived hypoxic small extracellular vesicles promote endothelial cell migration and tube formation via ALS2/Rab5/β‐catenin signaling DOI
Patricio Silva,

N. A. Hernandez,

Héctor Tapia

et al.

The FASEB Journal, Journal Year: 2024, Volume and Issue: 38(11)

Published: June 7, 2024

Abstract Tumor hypoxia has been associated with cancer progression, angiogenesis, and metastasis via modifications in the release cargo composition of extracellular vesicles secreted by tumor cells. Indeed, hypoxic are known to trigger a variety angiogenic responses different mechanisms. We recently showed that promotes endosomal signaling cells HIF‐1α‐dependent induction guanine exchange factor ALS2, which activates Rab5, leading downstream events involved cell migration invasion. Since Rab5‐dependent is required for endothelial we explored possibility small containing turn activate Rab5 recipient pro‐angiogenic properties. In doing so, found promoted ALS2 expression incorporation as within vesicles, subsequent transfer promoting migration, tube formation, activation. Consequently, ALS2‐containing increased early endosome size number cells, was followed sequestration components β‐catenin destruction complex compartments, stabilization nuclear localization β‐catenin. These converged target genes angiogenesis. Knockdown donor precluded its into preventing Rab5‐downstream responses, depended on activity ALS2. findings indicate vesicular hypoxia, Finally, these might explain how angiogenesis proceeds conditions.

Language: Английский

Unraveling the multifaceted roles of extracellular vesicles in bladder cancer: diagnostic insights and therapeutic opportunities DOI Creative Commons

Kaibin Huang,

Chen Yang,

Yanfang Xu

et al.

Frontiers in Oncology, Journal Year: 2025, Volume and Issue: 15

Published: May 6, 2025

Bladder cancer, predominantly urothelial carcinoma, is a global health issue with increasing incidences and mortality. It poses significant diagnostic therapeutic challenges due to its molecular heterogeneity the limitations of current detection methods. Extracellular vesicles (EVs), including exosomes, play crucial role in intercellular communication have emerged as potential biomarkers agents bladder cancer. This review focuses on multifaceted roles EVs cancer biology, their biomarkers, use strategies. We discuss how reflect subtypes participate metabolic reprogramming angiogenesis, modulate cellular behavior. The also highlights advances proteomic analysis urinary tissue-exudative EVs, identifying specific proteins RNAs that could serve non-invasive markers. Furthermore, we explore innovative natural nanocarriers for drug delivery treatment, demonstrating enhance efficacy chemotherapy selectively target cells. integration EV-based diagnostics traditional methods lead more personalized effective management, emphasizing need further research clinical validation.

Language: Английский

Citations

0
Exosome, the glass slipper for Cinderella of cancer—bladder cancer? DOI Creative Commons
Yuanyuan Yang, Lin-Tao Miao, Yuchao Lu

et al.

Journal of Nanobiotechnology, Journal Year: 2023, Volume and Issue: 21(1)

Published: Oct. 7, 2023

Language: Английский

Citations

9
Insight into extracellular vesicles in vascular diseases: intercellular communication role and clinical application potential DOI Creative Commons
Wenxiu Liu,

Meiqi Jin,

Qiuyan Chen

et al.

Cell Communication and Signaling, Journal Year: 2023, Volume and Issue: 21(1)

Published: Oct. 31, 2023

Abstract Background Cells have been increasingly known to release extracellular vesicles (EVs) the environment under physiological and pathological conditions. A plethora of studies revealed that EVs contain cell-derived biomolecules are found in circulation, thereby implicating them molecular trafficking between cells. Furthermore, an effect on function disease development serve as biomarkers. Main body Given close association EV circulation vascular disease, this review aims provide a brief introduction EVs, with specific focus cargoes participating mechanisms, diagnosis, engineering, clinical potential, highlight emerging evidence suggesting promising targets diseases. Despite expansion research field, some noticeable limitations remain for translational research. Conclusion This makes novel contribution summary recent advances perspective future

Language: Английский

Citations

8
Protein–Protein Interactions of Seryl-tRNA Synthetases with Emphasis on Human Counterparts and Their Connection to Health and Disease DOI Creative Commons
Morana Dulić, Vlatka Godinić Mikulčić,

Mario Kekez

et al.

Life, Journal Year: 2024, Volume and Issue: 14(1), P. 124 - 124

Published: Jan. 15, 2024

Seryl-tRNA synthetases (SerRSs), members of the aminoacyl-tRNA synthetase family, interact with diverse proteins, enabling SerRSs to enhance their role in translation genetic message or perform alternative functions cellular processes beyond translation. Atypical archaeal SerRS interacts arginyl-tRNA and proteins ribosomal P-stalk optimize through tRNA channeling. The complex between yeast peroxin Pex21p provides a connection peroxisome function. partnership Arabidopsis BEN1 indicates link brassinosteroid metabolism may be relevant plant stress response mechanisms. In Drosophila, unusual heterodimeric mitochondrial coordinates replication via interaction LON protease. Evolutionarily conserved interactions human m3C32 methyltransferases indicate coordination modification aminoacylation cytosol mitochondria. Human cytosolic is hub protein connecting vascular development, angiogenesis, lipogenesis, telomere maintenance. When translocated nucleus, acts as master negative regulator VEGFA gene expression. alone YY1 SIRT2 competes activating transcription factors NFκB1 c-Myc, resulting balanced expression important for proper development angiogenesis. hypoxia, phosphorylation diminishes its binding promoter, while lack nutrients triggers glycosylation, reducing nuclear localization. Additionally, binds telomeric DNA cooperates shelterin POT1 regulate length senescence. As an antitumor antiangiogenic factor, appears promising drug target therapeutic agent treating cancer, cardiovascular diseases, possibly obesity aging.

Language: Английский

Citations

2
Tumor‐derived hypoxic small extracellular vesicles promote endothelial cell migration and tube formation via ALS2/Rab5/β‐catenin signaling DOI
Patricio Silva,

N. A. Hernandez,

Héctor Tapia

et al.

The FASEB Journal, Journal Year: 2024, Volume and Issue: 38(11)

Published: June 7, 2024

Abstract Tumor hypoxia has been associated with cancer progression, angiogenesis, and metastasis via modifications in the release cargo composition of extracellular vesicles secreted by tumor cells. Indeed, hypoxic are known to trigger a variety angiogenic responses different mechanisms. We recently showed that promotes endosomal signaling cells HIF‐1α‐dependent induction guanine exchange factor ALS2, which activates Rab5, leading downstream events involved cell migration invasion. Since Rab5‐dependent is required for endothelial we explored possibility small containing turn activate Rab5 recipient pro‐angiogenic properties. In doing so, found promoted ALS2 expression incorporation as within vesicles, subsequent transfer promoting migration, tube formation, activation. Consequently, ALS2‐containing increased early endosome size number cells, was followed sequestration components β‐catenin destruction complex compartments, stabilization nuclear localization β‐catenin. These converged target genes angiogenesis. Knockdown donor precluded its into preventing Rab5‐downstream responses, depended on activity ALS2. findings indicate vesicular hypoxia, Finally, these might explain how angiogenesis proceeds conditions.

Language: Английский

Citations

2